Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells

Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bruno R. B. Pires, Renata Binato, Gerson M. Ferreira, Stephany Corrêa, Bárbara Du Rocher, Daniel Bulzico, Susanne Crocamo, Everton Cruz dos Santos, Luize G. Lima, Eliana Abdelhay
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	breast cancer HER2 Twist1 IL17 gene expression Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1b4d8a45346f4af1bb6c41e4b7ebec1f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b4d8a45346f4af1bb6c41e4b7ebec1f
record_format	dspace
spelling	oai:doaj.org-article:1b4d8a45346f4af1bb6c41e4b7ebec1f2021-11-25T17:53:47ZTwist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells10.3390/ijms2222121441422-00671661-6596https://doaj.org/article/1b4d8a45346f4af1bb6c41e4b7ebec1f2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12144https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist1. However, its role beyond EMT in BC subtypes remains unclear. Our study aimed to investigate the role of Twist1, beyond EMT, in the molecular subtypes of BC. In patients, we observed the overexpression of TWIST1 in the HER2+ group. The silencing of TWIST1 in HER2+ BC cells resulted in the upregulation of 138 genes and the downregulation of 174 genes compared to control cells in a microarray assay. In silico analysis revealed correlations between Twist1 and important biological processes such as the Th17-mediated immune response, suggesting that Twist1 could be relevant for IL-17 signaling in HER2+ BC. IL-17 signaling was then examined, and it was shown that TWIST1 knockdown caused the downregulation of leading members of IL-17 signaling pathway. Taken together, our findings suggest that Twist1 plays a role on IL-17 signaling in HER2+ BC.Bruno R. B. PiresRenata BinatoGerson M. FerreiraStephany CorrêaBárbara Du RocherDaniel BulzicoSusanne CrocamoEverton Cruz dos SantosLuize G. LimaEliana AbdelhayMDPI AGarticlebreast cancerHER2Twist1IL17gene expressionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12144, p 12144 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer HER2 Twist1 IL17 gene expression Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	breast cancer HER2 Twist1 IL17 gene expression Biology (General) QH301-705.5 Chemistry QD1-999 Bruno R. B. Pires Renata Binato Gerson M. Ferreira Stephany Corrêa Bárbara Du Rocher Daniel Bulzico Susanne Crocamo Everton Cruz dos Santos Luize G. Lima Eliana Abdelhay Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
description	Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist1. However, its role beyond EMT in BC subtypes remains unclear. Our study aimed to investigate the role of Twist1, beyond EMT, in the molecular subtypes of BC. In patients, we observed the overexpression of TWIST1 in the HER2+ group. The silencing of TWIST1 in HER2+ BC cells resulted in the upregulation of 138 genes and the downregulation of 174 genes compared to control cells in a microarray assay. In silico analysis revealed correlations between Twist1 and important biological processes such as the Th17-mediated immune response, suggesting that Twist1 could be relevant for IL-17 signaling in HER2+ BC. IL-17 signaling was then examined, and it was shown that TWIST1 knockdown caused the downregulation of leading members of IL-17 signaling pathway. Taken together, our findings suggest that Twist1 plays a role on IL-17 signaling in HER2+ BC.
format	article
author	Bruno R. B. Pires Renata Binato Gerson M. Ferreira Stephany Corrêa Bárbara Du Rocher Daniel Bulzico Susanne Crocamo Everton Cruz dos Santos Luize G. Lima Eliana Abdelhay
author_facet	Bruno R. B. Pires Renata Binato Gerson M. Ferreira Stephany Corrêa Bárbara Du Rocher Daniel Bulzico Susanne Crocamo Everton Cruz dos Santos Luize G. Lima Eliana Abdelhay
author_sort	Bruno R. B. Pires
title	Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_short	Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_full	Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_fullStr	Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_full_unstemmed	Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_sort	twist1 influences the expression of leading members of the il-17 signaling pathway in her2-positive breast cancer cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1b4d8a45346f4af1bb6c41e4b7ebec1f
work_keys_str_mv	AT brunorbpires twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT renatabinato twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT gersonmferreira twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT stephanycorrea twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT barbaradurocher twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT danielbulzico twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT susannecrocamo twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT evertoncruzdossantos twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT luizeglima twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells AT elianaabdelhay twist1influencestheexpressionofleadingmembersoftheil17signalingpathwayinher2positivebreastcancercells
_version_	1718411848550711296

Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells

Ejemplares similares