Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.

An in vivo study was conducted to compare the enteric methane emissions and diversity of ruminal methanogens in cattle and buffaloes kept in the same environment and fed on the same diet. Six cattle and six buffaloes were fed on a similar diet comprising Napier (Pennisetum purpureum) green grass and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	P K Malik, S Trivedi, A Mohapatra, A P Kolte, V Sejian, R Bhatta, H Rahman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b503f6a4e8247439503954f8e1f9297
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b503f6a4e8247439503954f8e1f9297
record_format	dspace
spelling	oai:doaj.org-article:1b503f6a4e8247439503954f8e1f92972021-12-02T20:18:21ZComparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.1932-620310.1371/journal.pone.0256048https://doaj.org/article/1b503f6a4e8247439503954f8e1f92972021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256048https://doaj.org/toc/1932-6203An in vivo study was conducted to compare the enteric methane emissions and diversity of ruminal methanogens in cattle and buffaloes kept in the same environment and fed on the same diet. Six cattle and six buffaloes were fed on a similar diet comprising Napier (Pennisetum purpureum) green grass and concentrate in 70:30. After 90 days of feeding, the daily enteric methane emissions were quantified by using the SF6 technique and ruminal fluid samples from animals were collected for the diversity analysis. The daily enteric methane emissions were significantly greater in cattle as compared to buffaloes; however, methane yields were not different between the two species. Methanogens were ranked at different taxonomic levels against the Rumen and Intestinal Methanogen-Database. The archaeal communities in both host species were dominated by the phylum Euryarchaeota; however, Crenarchaeota represented <1% of the total archaea. Methanogens affiliated with Methanobacteriales were most prominent and their proportion did not differ between the two hosts. Methanomicrobiales and Methanomassillicoccales constituted the second largest group of methanogens in cattle and buffaloes, respectively. Methanocellales (Methanocella arvoryza) were exclusively detected in the buffaloes. At the species level, Methanobrevibacter gottschalkii had the highest abundance (55-57%) in both the host species. The relative abundance of Methanobrevibacter wolinii between the two hosts differed significantly. Methanosarcinales, the acetoclastic methanogens were significantly greater in cattle than the buffaloes. It is concluded that the ruminal methane yield in cattle and buffaloes fed on the same diet did not differ. With the diet used in this study, there was a limited influence (<3.5%) of the host on the structure of the ruminal archaea community at the species level. Therefore, the methane mitigation strategies developed in either of the hosts should be effective in the other. Further studies are warranted to reveal the conjunctive effect of diet and geographical locations with the host on ruminal archaea community composition.P K MalikS TrivediA MohapatraA P KolteV SejianR BhattaH RahmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256048 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P K Malik S Trivedi A Mohapatra A P Kolte V Sejian R Bhatta H Rahman Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.
description	An in vivo study was conducted to compare the enteric methane emissions and diversity of ruminal methanogens in cattle and buffaloes kept in the same environment and fed on the same diet. Six cattle and six buffaloes were fed on a similar diet comprising Napier (Pennisetum purpureum) green grass and concentrate in 70:30. After 90 days of feeding, the daily enteric methane emissions were quantified by using the SF6 technique and ruminal fluid samples from animals were collected for the diversity analysis. The daily enteric methane emissions were significantly greater in cattle as compared to buffaloes; however, methane yields were not different between the two species. Methanogens were ranked at different taxonomic levels against the Rumen and Intestinal Methanogen-Database. The archaeal communities in both host species were dominated by the phylum Euryarchaeota; however, Crenarchaeota represented <1% of the total archaea. Methanogens affiliated with Methanobacteriales were most prominent and their proportion did not differ between the two hosts. Methanomicrobiales and Methanomassillicoccales constituted the second largest group of methanogens in cattle and buffaloes, respectively. Methanocellales (Methanocella arvoryza) were exclusively detected in the buffaloes. At the species level, Methanobrevibacter gottschalkii had the highest abundance (55-57%) in both the host species. The relative abundance of Methanobrevibacter wolinii between the two hosts differed significantly. Methanosarcinales, the acetoclastic methanogens were significantly greater in cattle than the buffaloes. It is concluded that the ruminal methane yield in cattle and buffaloes fed on the same diet did not differ. With the diet used in this study, there was a limited influence (<3.5%) of the host on the structure of the ruminal archaea community at the species level. Therefore, the methane mitigation strategies developed in either of the hosts should be effective in the other. Further studies are warranted to reveal the conjunctive effect of diet and geographical locations with the host on ruminal archaea community composition.
format	article
author	P K Malik S Trivedi A Mohapatra A P Kolte V Sejian R Bhatta H Rahman
author_facet	P K Malik S Trivedi A Mohapatra A P Kolte V Sejian R Bhatta H Rahman
author_sort	P K Malik
title	Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.
title_short	Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.
title_full	Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.
title_fullStr	Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.
title_full_unstemmed	Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.
title_sort	comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1b503f6a4e8247439503954f8e1f9297
work_keys_str_mv	AT pkmalik comparisonofentericmethaneyieldanddiversityofruminalmethanogensincattleandbuffaloesfedonthesamediet AT strivedi comparisonofentericmethaneyieldanddiversityofruminalmethanogensincattleandbuffaloesfedonthesamediet AT amohapatra comparisonofentericmethaneyieldanddiversityofruminalmethanogensincattleandbuffaloesfedonthesamediet AT apkolte comparisonofentericmethaneyieldanddiversityofruminalmethanogensincattleandbuffaloesfedonthesamediet AT vsejian comparisonofentericmethaneyieldanddiversityofruminalmethanogensincattleandbuffaloesfedonthesamediet AT rbhatta comparisonofentericmethaneyieldanddiversityofruminalmethanogensincattleandbuffaloesfedonthesamediet AT hrahman comparisonofentericmethaneyieldanddiversityofruminalmethanogensincattleandbuffaloesfedonthesamediet
_version_	1718374272039124992

Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet.

Ejemplares similares