Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.

Assessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromona...

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Autores principales:	Miguel L Grilo, Lélia Chambel, Tiago A Marques, Carla Sousa-Santos, Joana I Robalo, Manuela Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b5506acc85b4e1ca506eff4f546d817
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spelling	oai:doaj.org-article:1b5506acc85b4e1ca506eff4f546d8172021-12-02T20:18:36ZSympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.1932-620310.1371/journal.pone.0255850https://doaj.org/article/1b5506acc85b4e1ca506eff4f546d8172021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255850https://doaj.org/toc/1932-6203Assessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates' load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.Miguel L GriloLélia ChambelTiago A MarquesCarla Sousa-SantosJoana I RobaloManuela OliveiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255850 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miguel L Grilo Lélia Chambel Tiago A Marques Carla Sousa-Santos Joana I Robalo Manuela Oliveira Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.
description	Assessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates' load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.
format	article
author	Miguel L Grilo Lélia Chambel Tiago A Marques Carla Sousa-Santos Joana I Robalo Manuela Oliveira
author_facet	Miguel L Grilo Lélia Chambel Tiago A Marques Carla Sousa-Santos Joana I Robalo Manuela Oliveira
author_sort	Miguel L Grilo
title	Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.
title_short	Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.
title_full	Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.
title_fullStr	Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.
title_full_unstemmed	Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.
title_sort	sympatric threatened iberian leuciscids exhibit differences in aeromonas diversity and skin lesions' prevalence.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1b5506acc85b4e1ca506eff4f546d817
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Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence.

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