Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types

Abstract. Ekowati N, Mumpuni A, Ratnaningtyas NI, Maharning A. 2020. Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types. Biodiversitas 21: 5865-5871. Schizophyllum commune Fr.is a potential fungus utilized as a drug...

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Autores principales:	Nuraeni Ekowati, ARIS MUMPUNI, NUNIEK INA RATNANINGTYAS, ARDHINI RIN MAHARNING
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bioactive compounds, cancer cell lines, cytotoxic test, schizophyllum commune Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1b560737f00f4284b7f8cf019b442a9b
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spelling	oai:doaj.org-article:1b560737f00f4284b7f8cf019b442a9b2021-11-22T00:52:00ZCompounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types1412-033X2085-472210.13057/biodiv/d211251https://doaj.org/article/1b560737f00f4284b7f8cf019b442a9b2020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7001https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ekowati N, Mumpuni A, Ratnaningtyas NI, Maharning A. 2020. Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types. Biodiversitas 21: 5865-5871. Schizophyllum commune Fr.is a potential fungus utilized as a drug, including a cancer drug expectant. In Indonesia, the fungus' capability to suppress the growth of certain types of cancer cells has not been widely reported. The purposes of this study were (i) to determine the bioactive compound group from S. commune using organic solvents chloroform and ethyl acetate, (ii) to determine the IC50 values ??of S. commune bioactive compounds using cancer cell lines HeLa, MCF7, T47D and WiDr, (iii) to find out the mechanism of apoptosis. The research was carried out experimentally using a pure culture of four types of cancer cell lines. Anticancer activity test was conducted by a cytotoxic test using MTT [3- (4,5- dimethylthiazol -2-yl) -2.5-diphenyl tetrazolium bromide], and apoptosis test. Data analysis was performed with linear regression analysis, and the apoptosis test was analyzed descriptively. The results showed that the bioactive compounds produced by S. commune are alkaloids, terpenoids, and flavonoids. S. commune bioactive compounds potentially inhibit the four cancer cell lines used with IC50 values ??ranging from 5.44 to 420.48 ug/mL. Best result was obtained from S. commune extract of chloroform in T47D cells with IC50 = 5.44 ug/mL and are able to induce apoptosis.Nuraeni EkowatiARIS MUMPUNINUNIEK INA RATNANINGTYASARDHINI RIN MAHARNINGMBI & UNS Soloarticlebioactive compounds, cancer cell lines, cytotoxic test, schizophyllum communeBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioactive compounds, cancer cell lines, cytotoxic test, schizophyllum commune Biology (General) QH301-705.5
spellingShingle	bioactive compounds, cancer cell lines, cytotoxic test, schizophyllum commune Biology (General) QH301-705.5 Nuraeni Ekowati ARIS MUMPUNI NUNIEK INA RATNANINGTYAS ARDHINI RIN MAHARNING Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types
description	Abstract. Ekowati N, Mumpuni A, Ratnaningtyas NI, Maharning A. 2020. Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types. Biodiversitas 21: 5865-5871. Schizophyllum commune Fr.is a potential fungus utilized as a drug, including a cancer drug expectant. In Indonesia, the fungus' capability to suppress the growth of certain types of cancer cells has not been widely reported. The purposes of this study were (i) to determine the bioactive compound group from S. commune using organic solvents chloroform and ethyl acetate, (ii) to determine the IC50 values ??of S. commune bioactive compounds using cancer cell lines HeLa, MCF7, T47D and WiDr, (iii) to find out the mechanism of apoptosis. The research was carried out experimentally using a pure culture of four types of cancer cell lines. Anticancer activity test was conducted by a cytotoxic test using MTT [3- (4,5- dimethylthiazol -2-yl) -2.5-diphenyl tetrazolium bromide], and apoptosis test. Data analysis was performed with linear regression analysis, and the apoptosis test was analyzed descriptively. The results showed that the bioactive compounds produced by S. commune are alkaloids, terpenoids, and flavonoids. S. commune bioactive compounds potentially inhibit the four cancer cell lines used with IC50 values ??ranging from 5.44 to 420.48 ug/mL. Best result was obtained from S. commune extract of chloroform in T47D cells with IC50 = 5.44 ug/mL and are able to induce apoptosis.
format	article
author	Nuraeni Ekowati ARIS MUMPUNI NUNIEK INA RATNANINGTYAS ARDHINI RIN MAHARNING
author_facet	Nuraeni Ekowati ARIS MUMPUNI NUNIEK INA RATNANINGTYAS ARDHINI RIN MAHARNING
author_sort	Nuraeni Ekowati
title	Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types
title_short	Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types
title_full	Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types
title_fullStr	Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types
title_full_unstemmed	Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types
title_sort	compounds detection and inhibition activity of chloroform and ethyl acetate extracts of schizophyllum commune on some cancer cell types
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/1b560737f00f4284b7f8cf019b442a9b
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Compounds detection and inhibition activity of chloroform and ethyl acetate extracts of Schizophyllum commune on some cancer cell types

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