Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism

Abstract Cage layer osteoporosis (CLO) is a common bone metabolism disease in the breeding industry of China. However, effective prevention for CLO has not been developed. Icariin (ICA), the main bioactive component of the Chinese herb Epimedium, has been shown to have good therapeutic effects on bo...

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Autores principales:	Zhengwang Yu, Jie Huang, Zhongxin Zhou
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Osteoporosis Laying hen Bone mineral density Icariin Metabolomics Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/1b5aafb53b2f44f6860302470cd13e71
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spelling	oai:doaj.org-article:1b5aafb53b2f44f6860302470cd13e712021-11-14T12:06:05ZIcariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism10.1186/s44149-021-00001-z2731-0442https://doaj.org/article/1b5aafb53b2f44f6860302470cd13e712021-04-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00001-zhttps://doaj.org/toc/2731-0442Abstract Cage layer osteoporosis (CLO) is a common bone metabolism disease in the breeding industry of China. However, effective prevention for CLO has not been developed. Icariin (ICA), the main bioactive component of the Chinese herb Epimedium, has been shown to have good therapeutic effects on bone-related diseases. In this study, the effects of ICA were further evaluated in a low-calcium diet-induced CLO, and a serum metabolomics assay was performed to understand the underlying mechanisms. A total of 144 31-wk-old Lohmann pink-shell laying hens were randomly allocated to 4 groups with 6 replicates of 6 hens per replicate. The 4 dietary treatment groups consisted of a basal diet (3.5% calcium), a low-calcium diet (2.0% calcium), and a low-calcium diet supplemented with 0.5 or 2.0 g/kg ICA. The results showed that ICA exerted good osteoprotective effects on low-calcium diet-induced CLO. ICA significantly increased femur bone mineral density, improved bone microstructure, decreased bone metabolic level, and upregulated mRNA expression of bone formation genes in femoral bone tissue. Serum untargeted metabolomics analysis showed that 8 metabolite levels were significantly changed after ICA treatment, including increased contents of 7-dehydrocholesterol, 7-oxocholesterol, desmosterol, PC (18:1(9Z)/18:1(9Z)), PS (18:0/18:1(9Z)), N,N-dimethylaniline and 2-hydroxy-butanoic acid and decreased N2,N2-dimethylguanosine. Metabolic pathway analysis based on the above 8 metabolites indicated that ICA mainly perturbed steroid biosynthesis and glycerophospholipid metabolism. These findings suggest that ICA can effectively prevent bone loss in low-calcium diet-induced CLO by mediating steroid biosynthesis and glycerophospholipid metabolism and provide new information for the regulation of bone metabolic diseases.Zhengwang YuJie HuangZhongxin ZhouBMCarticleOsteoporosisLaying henBone mineral densityIcariinMetabolomicsVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteoporosis Laying hen Bone mineral density Icariin Metabolomics Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Osteoporosis Laying hen Bone mineral density Icariin Metabolomics Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Zhengwang Yu Jie Huang Zhongxin Zhou Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism
description	Abstract Cage layer osteoporosis (CLO) is a common bone metabolism disease in the breeding industry of China. However, effective prevention for CLO has not been developed. Icariin (ICA), the main bioactive component of the Chinese herb Epimedium, has been shown to have good therapeutic effects on bone-related diseases. In this study, the effects of ICA were further evaluated in a low-calcium diet-induced CLO, and a serum metabolomics assay was performed to understand the underlying mechanisms. A total of 144 31-wk-old Lohmann pink-shell laying hens were randomly allocated to 4 groups with 6 replicates of 6 hens per replicate. The 4 dietary treatment groups consisted of a basal diet (3.5% calcium), a low-calcium diet (2.0% calcium), and a low-calcium diet supplemented with 0.5 or 2.0 g/kg ICA. The results showed that ICA exerted good osteoprotective effects on low-calcium diet-induced CLO. ICA significantly increased femur bone mineral density, improved bone microstructure, decreased bone metabolic level, and upregulated mRNA expression of bone formation genes in femoral bone tissue. Serum untargeted metabolomics analysis showed that 8 metabolite levels were significantly changed after ICA treatment, including increased contents of 7-dehydrocholesterol, 7-oxocholesterol, desmosterol, PC (18:1(9Z)/18:1(9Z)), PS (18:0/18:1(9Z)), N,N-dimethylaniline and 2-hydroxy-butanoic acid and decreased N2,N2-dimethylguanosine. Metabolic pathway analysis based on the above 8 metabolites indicated that ICA mainly perturbed steroid biosynthesis and glycerophospholipid metabolism. These findings suggest that ICA can effectively prevent bone loss in low-calcium diet-induced CLO by mediating steroid biosynthesis and glycerophospholipid metabolism and provide new information for the regulation of bone metabolic diseases.
format	article
author	Zhengwang Yu Jie Huang Zhongxin Zhou
author_facet	Zhengwang Yu Jie Huang Zhongxin Zhou
author_sort	Zhengwang Yu
title	Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism
title_short	Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism
title_full	Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism
title_fullStr	Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism
title_full_unstemmed	Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism
title_sort	icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1b5aafb53b2f44f6860302470cd13e71
work_keys_str_mv	AT zhengwangyu icariinprotectsagainstcagelayerosteoporosisbyinterveninginsteroidbiosynthesisandglycerophospholipidmetabolism AT jiehuang icariinprotectsagainstcagelayerosteoporosisbyinterveninginsteroidbiosynthesisandglycerophospholipidmetabolism AT zhongxinzhou icariinprotectsagainstcagelayerosteoporosisbyinterveninginsteroidbiosynthesisandglycerophospholipidmetabolism
_version_	1718429452930646016

Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism

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