Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor

The environmental release of mercury is continuously increasing with high degree of mobility, transformation and amplified toxicity. Improving remediation strategies is becoming increasingly important to achieve more stringent environmental safety standards. This study develops a laboratory-scale re...

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Autores principales:	Asifa Farooqi, Ghufranud Din, Rameesha Hayat, Malik Badshah, Samiullah Khan, Aamer Ali Shah
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	bacillus nealsonii bioreactor bioremediation mercuric reductase enzyme mercury transformation Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/1b5d09356b084fcdbe96f398ad58518e
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spelling	oai:doaj.org-article:1b5d09356b084fcdbe96f398ad58518e2021-11-06T11:01:59ZCharacterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor0273-12231996-973210.2166/wst.2021.122https://doaj.org/article/1b5d09356b084fcdbe96f398ad58518e2021-05-01T00:00:00Zhttp://wst.iwaponline.com/content/83/9/2287https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732The environmental release of mercury is continuously increasing with high degree of mobility, transformation and amplified toxicity. Improving remediation strategies is becoming increasingly important to achieve more stringent environmental safety standards. This study develops a laboratory-scale reactor for bioremediation of aqueous mercury using a biofilm-producing bacterial strain, KBH10, isolated from mercury-polluted soil. The strain was found resistant to 80 mg/L of HgCl2 and identified as Bacillus nealsonii via 16S rRNA gene sequence analysis. The strain KBH10 was characterized for optimum growth parameters and its mercury biotransformation potential was validated through mercuric reductase assay. A packed-bed column bioreactor was designed for biofilm-mediated mercury removal from artificially contaminated water and residual mercury was estimated. Strain KBH10 could grow at a range of temperature (20–50 °C) and pH (6.0–9.0) with optimum temperature established at 30 °C and pH 7.0. The optimum mercuric reductase activity (77.8 ± 1.7 U/mg) was reported at 30 °C and was stable at a temperature range of 20–50 °C. The residual mercury analysis of artificially contaminated water indicated 60.6 ± 1.5% reduction in mercury content within 5 h of exposure. This regenerative process of biofilm-mediated mercury removal in a packed-bed column bioreactor can provide new insight into its potential use in mercury bioremediation. HIGHLIGHTS Bioaccumulation of aqueous mercury in the food web is a matter of grave concern.; Enzymatic reduction of mercury by resistant bacteria reduces its potential toxicity.; Bacillus nealsonii possess the inbuilt mechanisms of necessary adaptation.; Mercury content was reduced up to 60.6 ± 1.5% within 5 h of exposure in packed-bed bioreactor.; Biofilm-mediated mercury remediation is efficient, regenerative and cost-effective.;Asifa FarooqiGhufranud DinRameesha HayatMalik BadshahSamiullah KhanAamer Ali ShahIWA Publishingarticlebacillus nealsoniibioreactorbioremediationmercuric reductase enzymemercurytransformationEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 9, Pp 2287-2295 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus nealsonii bioreactor bioremediation mercuric reductase enzyme mercury transformation Environmental technology. Sanitary engineering TD1-1066
spellingShingle	bacillus nealsonii bioreactor bioremediation mercuric reductase enzyme mercury transformation Environmental technology. Sanitary engineering TD1-1066 Asifa Farooqi Ghufranud Din Rameesha Hayat Malik Badshah Samiullah Khan Aamer Ali Shah Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor
description	The environmental release of mercury is continuously increasing with high degree of mobility, transformation and amplified toxicity. Improving remediation strategies is becoming increasingly important to achieve more stringent environmental safety standards. This study develops a laboratory-scale reactor for bioremediation of aqueous mercury using a biofilm-producing bacterial strain, KBH10, isolated from mercury-polluted soil. The strain was found resistant to 80 mg/L of HgCl2 and identified as Bacillus nealsonii via 16S rRNA gene sequence analysis. The strain KBH10 was characterized for optimum growth parameters and its mercury biotransformation potential was validated through mercuric reductase assay. A packed-bed column bioreactor was designed for biofilm-mediated mercury removal from artificially contaminated water and residual mercury was estimated. Strain KBH10 could grow at a range of temperature (20–50 °C) and pH (6.0–9.0) with optimum temperature established at 30 °C and pH 7.0. The optimum mercuric reductase activity (77.8 ± 1.7 U/mg) was reported at 30 °C and was stable at a temperature range of 20–50 °C. The residual mercury analysis of artificially contaminated water indicated 60.6 ± 1.5% reduction in mercury content within 5 h of exposure. This regenerative process of biofilm-mediated mercury removal in a packed-bed column bioreactor can provide new insight into its potential use in mercury bioremediation. HIGHLIGHTS Bioaccumulation of aqueous mercury in the food web is a matter of grave concern.; Enzymatic reduction of mercury by resistant bacteria reduces its potential toxicity.; Bacillus nealsonii possess the inbuilt mechanisms of necessary adaptation.; Mercury content was reduced up to 60.6 ± 1.5% within 5 h of exposure in packed-bed bioreactor.; Biofilm-mediated mercury remediation is efficient, regenerative and cost-effective.;
format	article
author	Asifa Farooqi Ghufranud Din Rameesha Hayat Malik Badshah Samiullah Khan Aamer Ali Shah
author_facet	Asifa Farooqi Ghufranud Din Rameesha Hayat Malik Badshah Samiullah Khan Aamer Ali Shah
author_sort	Asifa Farooqi
title	Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor
title_short	Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor
title_full	Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor
title_fullStr	Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor
title_full_unstemmed	Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor
title_sort	characterization of bacillus nealsonii strain kbh10 capable of reducing aqueous mercury in laboratory-scale reactor
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/1b5d09356b084fcdbe96f398ad58518e
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Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor

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