Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung.
Neonates, including mice and humans, are highly susceptible to cytomegalovirus (CMV) infection. However, many aspects of neonatal CMV infections such as viral cell tropism, spatio-temporal distribution of the pathogen as well as genesis of antiviral immunity are unknown. With the use of reporter mut...
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Public Library of Science (PLoS)
2013
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oai:doaj.org-article:1b6b9c02372e45b19a3729b5c0d6b8e12021-11-18T06:07:14ZNodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung.1553-73661553-737410.1371/journal.ppat.1003828https://doaj.org/article/1b6b9c02372e45b19a3729b5c0d6b8e12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24348257/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Neonates, including mice and humans, are highly susceptible to cytomegalovirus (CMV) infection. However, many aspects of neonatal CMV infections such as viral cell tropism, spatio-temporal distribution of the pathogen as well as genesis of antiviral immunity are unknown. With the use of reporter mutants of the murine cytomegalovirus (MCMV) we identified the lung as a primary target of mucosal infection in neonatal mice. Comparative analysis of neonatal and adult mice revealed a delayed control of virus replication in the neonatal lung mucosa explaining the pronounced systemic infection and disease in neonates. This phenomenon was supplemented by a delayed expansion of CD8(+) T cell clones recognizing the viral protein M45 in neonates. We detected viral infection at the single-cell level and observed myeloid cells forming "nodular inflammatory foci" (NIF) in the neonatal lung. Co-localization of infected cells within NIFs was associated with their disruption and clearance of the infection. By 2-photon microscopy, we characterized how neonatal antigen-presenting cells (APC) interacted with T cells and induced mature adaptive immune responses within such NIFs. We thus define NIFs of the neonatal lung as niches for prolonged MCMV replication and T cell priming but also as sites of infection control.Felix R StahlKatrin HellerStephan HalleKirsten A KeyserAndreas BuscheAnja MarquardtKaren WagnerJasmin BoelterYvonne BischoffElisabeth KremmerRamon ArensMartin MesserleReinhold FörsterPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 12, p e1003828 (2013) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Felix R Stahl Katrin Heller Stephan Halle Kirsten A Keyser Andreas Busche Anja Marquardt Karen Wagner Jasmin Boelter Yvonne Bischoff Elisabeth Kremmer Ramon Arens Martin Messerle Reinhold Förster Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. |
| description |
Neonates, including mice and humans, are highly susceptible to cytomegalovirus (CMV) infection. However, many aspects of neonatal CMV infections such as viral cell tropism, spatio-temporal distribution of the pathogen as well as genesis of antiviral immunity are unknown. With the use of reporter mutants of the murine cytomegalovirus (MCMV) we identified the lung as a primary target of mucosal infection in neonatal mice. Comparative analysis of neonatal and adult mice revealed a delayed control of virus replication in the neonatal lung mucosa explaining the pronounced systemic infection and disease in neonates. This phenomenon was supplemented by a delayed expansion of CD8(+) T cell clones recognizing the viral protein M45 in neonates. We detected viral infection at the single-cell level and observed myeloid cells forming "nodular inflammatory foci" (NIF) in the neonatal lung. Co-localization of infected cells within NIFs was associated with their disruption and clearance of the infection. By 2-photon microscopy, we characterized how neonatal antigen-presenting cells (APC) interacted with T cells and induced mature adaptive immune responses within such NIFs. We thus define NIFs of the neonatal lung as niches for prolonged MCMV replication and T cell priming but also as sites of infection control. |
| format |
article |
| author |
Felix R Stahl Katrin Heller Stephan Halle Kirsten A Keyser Andreas Busche Anja Marquardt Karen Wagner Jasmin Boelter Yvonne Bischoff Elisabeth Kremmer Ramon Arens Martin Messerle Reinhold Förster |
| author_facet |
Felix R Stahl Katrin Heller Stephan Halle Kirsten A Keyser Andreas Busche Anja Marquardt Karen Wagner Jasmin Boelter Yvonne Bischoff Elisabeth Kremmer Ramon Arens Martin Messerle Reinhold Förster |
| author_sort |
Felix R Stahl |
| title |
Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. |
| title_short |
Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. |
| title_full |
Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. |
| title_fullStr |
Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. |
| title_full_unstemmed |
Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. |
| title_sort |
nodular inflammatory foci are sites of t cell priming and control of murine cytomegalovirus infection in the neonatal lung. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/1b6b9c02372e45b19a3729b5c0d6b8e1 |
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