Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway

Abstract Wildfire smoke induces acute pulmonary distress and is of particular concern to risk groups such as the sick and elderly. Wood smoke (WS) contains many of the same toxic compounds as those found in cigarette smoke (CS) including polycyclic aromatic hydrocarbons, carbon monoxide, and free ra...

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Autores principales:	Matthew R. Zeglinski, Christopher T. Turner, Rui Zeng, Carley Schwartz, Stephanie Santacruz, Megan A. Pawluk, Hongyan Zhao, Arthur W. H. Chan, Christopher Carlsten, David J. Granville
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b6c2b4dbf5d4315845a64aa81cc9869
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spelling	oai:doaj.org-article:1b6c2b4dbf5d4315845a64aa81cc98692021-12-02T15:08:47ZSoluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway10.1038/s41598-019-46400-82045-2322https://doaj.org/article/1b6c2b4dbf5d4315845a64aa81cc98692019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46400-8https://doaj.org/toc/2045-2322Abstract Wildfire smoke induces acute pulmonary distress and is of particular concern to risk groups such as the sick and elderly. Wood smoke (WS) contains many of the same toxic compounds as those found in cigarette smoke (CS) including polycyclic aromatic hydrocarbons, carbon monoxide, and free radicals. CS is a well-established risk factor for respiratory diseases such as asthma and COPD. Limited studies investigating the biological effects of WS on the airway epithelium have been performed. Using a cell culture-based model, we assessed the effects of a WS-infused solution on alveolar epithelial barrier function, cell migration, and survival. The average geometric mean of particles in the WS was 178 nm. GC/MS analysis of the WS solution identified phenolic and cellulosic compounds. WS exposure resulted in a significant reduction in barrier function, which peaked after 24 hours of continuous exposure. The junctional protein E-cadherin showed a prominent reduction in response to increasing concentrations of WS. Furthermore, WS significantly repressed cell migration following injury to the cell monolayer. There was no difference in cell viability following WS exposure. Mechanistically, WS exposure induced activation of the p44/42, but not p38, MAPK signaling pathway, and inhibition of p44/42 phosphorylation prevented the disruption of barrier function and loss of E-cadherin staining. Thus, WS may contribute to the breakdown of alveolar structure and function through a p44/42 MAPK-dependent pathway and may lead to the development and/or exacerbation of respiratory pathologies with chronic exposure.Matthew R. ZeglinskiChristopher T. TurnerRui ZengCarley SchwartzStephanie SantacruzMegan A. PawlukHongyan ZhaoArthur W. H. ChanChristopher CarlstenDavid J. GranvilleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthew R. Zeglinski Christopher T. Turner Rui Zeng Carley Schwartz Stephanie Santacruz Megan A. Pawluk Hongyan Zhao Arthur W. H. Chan Christopher Carlsten David J. Granville Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway
description	Abstract Wildfire smoke induces acute pulmonary distress and is of particular concern to risk groups such as the sick and elderly. Wood smoke (WS) contains many of the same toxic compounds as those found in cigarette smoke (CS) including polycyclic aromatic hydrocarbons, carbon monoxide, and free radicals. CS is a well-established risk factor for respiratory diseases such as asthma and COPD. Limited studies investigating the biological effects of WS on the airway epithelium have been performed. Using a cell culture-based model, we assessed the effects of a WS-infused solution on alveolar epithelial barrier function, cell migration, and survival. The average geometric mean of particles in the WS was 178 nm. GC/MS analysis of the WS solution identified phenolic and cellulosic compounds. WS exposure resulted in a significant reduction in barrier function, which peaked after 24 hours of continuous exposure. The junctional protein E-cadherin showed a prominent reduction in response to increasing concentrations of WS. Furthermore, WS significantly repressed cell migration following injury to the cell monolayer. There was no difference in cell viability following WS exposure. Mechanistically, WS exposure induced activation of the p44/42, but not p38, MAPK signaling pathway, and inhibition of p44/42 phosphorylation prevented the disruption of barrier function and loss of E-cadherin staining. Thus, WS may contribute to the breakdown of alveolar structure and function through a p44/42 MAPK-dependent pathway and may lead to the development and/or exacerbation of respiratory pathologies with chronic exposure.
format	article
author	Matthew R. Zeglinski Christopher T. Turner Rui Zeng Carley Schwartz Stephanie Santacruz Megan A. Pawluk Hongyan Zhao Arthur W. H. Chan Christopher Carlsten David J. Granville
author_facet	Matthew R. Zeglinski Christopher T. Turner Rui Zeng Carley Schwartz Stephanie Santacruz Megan A. Pawluk Hongyan Zhao Arthur W. H. Chan Christopher Carlsten David J. Granville
author_sort	Matthew R. Zeglinski
title	Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway
title_short	Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway
title_full	Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway
title_fullStr	Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway
title_full_unstemmed	Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway
title_sort	soluble wood smoke extract promotes barrier dysfunction in alveolar epithelial cells through a mapk signaling pathway
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/1b6c2b4dbf5d4315845a64aa81cc9869
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Soluble Wood Smoke Extract Promotes Barrier Dysfunction in Alveolar Epithelial Cells through a MAPK Signaling Pathway

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