MicroRNA expression profiling of the porcine developing brain.

<h4>Background</h4>MicroRNAs are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. In particular, the developing brain contains an impressive diversity of...

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Autores principales:	Agnieszka Podolska, Bogumil Kaczkowski, Peter Kamp Busk, Rolf Søkilde, Thomas Litman, Merete Fredholm, Susanna Cirera
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b71f0c0a4a845f48770b80a3e78c41e
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spelling	oai:doaj.org-article:1b71f0c0a4a845f48770b80a3e78c41e2021-11-18T07:00:44ZMicroRNA expression profiling of the porcine developing brain.1932-620310.1371/journal.pone.0014494https://doaj.org/article/1b71f0c0a4a845f48770b80a3e78c41e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21253018/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. In particular, the developing brain contains an impressive diversity of microRNAs. Most microRNA expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. The domestic pig is considered to be an excellent, alternate, large mammal model for human-related neurological studies, due to its similarity in both brain development and the growth curve when compared to humans. Considering these similarities, studies examining microRNA expression during porcine brain development could potentially be used to predict the expression profile and role of microRNAs in the human brain.<h4>Methodology/principal findings</h4>MicroRNA expression profiling by use of microRNA microarrays and qPCR was performed on the porcine developing brain. Our results show that microRNA expression is regulated in a developmentally stage-specific, as well as a tissue-specific manner. Numerous developmental stage or tissue-specific microRNAs including, miR-17, miR-18a, miR-29c, miR-106a, miR-135a and b, miR-221 and miR-222 were found by microarray analysis. Expression profiles of selected candidates were confirmed by qPCR.<h4>Conclusions/significance</h4>The differential expression of specific microRNAs in fetal versus postnatal samples suggests that they likely play an important role in the regulation of developmental and physiological processes during brain development. The data presented here supports the notion that microRNAs act as post-transcriptional switches which may regulate gene expression when required.Agnieszka PodolskaBogumil KaczkowskiPeter Kamp BuskRolf SøkildeThomas LitmanMerete FredholmSusanna CireraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e14494 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agnieszka Podolska Bogumil Kaczkowski Peter Kamp Busk Rolf Søkilde Thomas Litman Merete Fredholm Susanna Cirera MicroRNA expression profiling of the porcine developing brain.
description	<h4>Background</h4>MicroRNAs are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. In particular, the developing brain contains an impressive diversity of microRNAs. Most microRNA expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. The domestic pig is considered to be an excellent, alternate, large mammal model for human-related neurological studies, due to its similarity in both brain development and the growth curve when compared to humans. Considering these similarities, studies examining microRNA expression during porcine brain development could potentially be used to predict the expression profile and role of microRNAs in the human brain.<h4>Methodology/principal findings</h4>MicroRNA expression profiling by use of microRNA microarrays and qPCR was performed on the porcine developing brain. Our results show that microRNA expression is regulated in a developmentally stage-specific, as well as a tissue-specific manner. Numerous developmental stage or tissue-specific microRNAs including, miR-17, miR-18a, miR-29c, miR-106a, miR-135a and b, miR-221 and miR-222 were found by microarray analysis. Expression profiles of selected candidates were confirmed by qPCR.<h4>Conclusions/significance</h4>The differential expression of specific microRNAs in fetal versus postnatal samples suggests that they likely play an important role in the regulation of developmental and physiological processes during brain development. The data presented here supports the notion that microRNAs act as post-transcriptional switches which may regulate gene expression when required.
format	article
author	Agnieszka Podolska Bogumil Kaczkowski Peter Kamp Busk Rolf Søkilde Thomas Litman Merete Fredholm Susanna Cirera
author_facet	Agnieszka Podolska Bogumil Kaczkowski Peter Kamp Busk Rolf Søkilde Thomas Litman Merete Fredholm Susanna Cirera
author_sort	Agnieszka Podolska
title	MicroRNA expression profiling of the porcine developing brain.
title_short	MicroRNA expression profiling of the porcine developing brain.
title_full	MicroRNA expression profiling of the porcine developing brain.
title_fullStr	MicroRNA expression profiling of the porcine developing brain.
title_full_unstemmed	MicroRNA expression profiling of the porcine developing brain.
title_sort	microrna expression profiling of the porcine developing brain.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1b71f0c0a4a845f48770b80a3e78c41e
work_keys_str_mv	AT agnieszkapodolska micrornaexpressionprofilingoftheporcinedevelopingbrain AT bogumilkaczkowski micrornaexpressionprofilingoftheporcinedevelopingbrain AT peterkampbusk micrornaexpressionprofilingoftheporcinedevelopingbrain AT rolfsøkilde micrornaexpressionprofilingoftheporcinedevelopingbrain AT thomaslitman micrornaexpressionprofilingoftheporcinedevelopingbrain AT meretefredholm micrornaexpressionprofilingoftheporcinedevelopingbrain AT susannacirera micrornaexpressionprofilingoftheporcinedevelopingbrain
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MicroRNA expression profiling of the porcine developing brain.

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