SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.

The SWI/SNF chromatin remodeling complexes regulate the transcription of many genes by remodeling nucleosomes at promoter regions. In Drosophila, SWI/SNF plays an important role in ecdysone-dependent transcription regulation. Studies in human cells suggest that Brahma (Brm), the ATPase subunit of SW...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anu Tyagi, Jessica Ryme, David Brodin, Ann Kristin Ostlund Farrants, Neus Visa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/1b74f349f41149bca6f25eea53472cb9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b74f349f41149bca6f25eea53472cb9
record_format	dspace
spelling	oai:doaj.org-article:1b74f349f41149bca6f25eea53472cb92021-11-25T05:53:27ZSWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.1553-73901553-740410.1371/journal.pgen.1000470https://doaj.org/article/1b74f349f41149bca6f25eea53472cb92009-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19424417/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The SWI/SNF chromatin remodeling complexes regulate the transcription of many genes by remodeling nucleosomes at promoter regions. In Drosophila, SWI/SNF plays an important role in ecdysone-dependent transcription regulation. Studies in human cells suggest that Brahma (Brm), the ATPase subunit of SWI/SNF, regulates alternative pre-mRNA splicing by modulating transcription elongation rates. We describe, here, experiments that study the association of Brm with transcribed genes in Chironomus tentans and Drosophila melanogaster, the purpose of which was to further elucidate the mechanisms by which Brm regulates pre-mRNA processing. We show that Brm becomes incorporated into nascent Balbiani ring pre-mRNPs co-transcriptionally and that the human Brm and Brg1 proteins are associated with RNPs. We have analyzed the expression profiles of D. melanogaster S2 cells in which the levels of individual SWI/SNF subunits have been reduced by RNA interference, and we show that depletion of SWI/SNF core subunits changes the relative abundance of alternative transcripts from a subset of genes. This observation, and the fact that a fraction of Brm is not associated with chromatin but with nascent pre-mRNPs, suggest that SWI/SNF affects pre-mRNA processing by acting at the RNA level. Ontology enrichment tests indicate that the genes that are regulated post-transcriptionally by SWI/SNF are mostly enzymes and transcription factors that regulate postembryonic developmental processes. In summary, the data suggest that SWI/SNF becomes incorporated into nascent pre-mRNPs and acts post-transcriptionally to regulate not only the amount of mRNA synthesized from a given promoter but also the type of alternative transcript produced.Anu TyagiJessica RymeDavid BrodinAnn Kristin Ostlund FarrantsNeus VisaPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 5, Iss 5, p e1000470 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Anu Tyagi Jessica Ryme David Brodin Ann Kristin Ostlund Farrants Neus Visa SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
description	The SWI/SNF chromatin remodeling complexes regulate the transcription of many genes by remodeling nucleosomes at promoter regions. In Drosophila, SWI/SNF plays an important role in ecdysone-dependent transcription regulation. Studies in human cells suggest that Brahma (Brm), the ATPase subunit of SWI/SNF, regulates alternative pre-mRNA splicing by modulating transcription elongation rates. We describe, here, experiments that study the association of Brm with transcribed genes in Chironomus tentans and Drosophila melanogaster, the purpose of which was to further elucidate the mechanisms by which Brm regulates pre-mRNA processing. We show that Brm becomes incorporated into nascent Balbiani ring pre-mRNPs co-transcriptionally and that the human Brm and Brg1 proteins are associated with RNPs. We have analyzed the expression profiles of D. melanogaster S2 cells in which the levels of individual SWI/SNF subunits have been reduced by RNA interference, and we show that depletion of SWI/SNF core subunits changes the relative abundance of alternative transcripts from a subset of genes. This observation, and the fact that a fraction of Brm is not associated with chromatin but with nascent pre-mRNPs, suggest that SWI/SNF affects pre-mRNA processing by acting at the RNA level. Ontology enrichment tests indicate that the genes that are regulated post-transcriptionally by SWI/SNF are mostly enzymes and transcription factors that regulate postembryonic developmental processes. In summary, the data suggest that SWI/SNF becomes incorporated into nascent pre-mRNPs and acts post-transcriptionally to regulate not only the amount of mRNA synthesized from a given promoter but also the type of alternative transcript produced.
format	article
author	Anu Tyagi Jessica Ryme David Brodin Ann Kristin Ostlund Farrants Neus Visa
author_facet	Anu Tyagi Jessica Ryme David Brodin Ann Kristin Ostlund Farrants Neus Visa
author_sort	Anu Tyagi
title	SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
title_short	SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
title_full	SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
title_fullStr	SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
title_full_unstemmed	SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
title_sort	swi/snf associates with nascent pre-mrnps and regulates alternative pre-mrna processing.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/1b74f349f41149bca6f25eea53472cb9
work_keys_str_mv	AT anutyagi swisnfassociateswithnascentpremrnpsandregulatesalternativepremrnaprocessing AT jessicaryme swisnfassociateswithnascentpremrnpsandregulatesalternativepremrnaprocessing AT davidbrodin swisnfassociateswithnascentpremrnpsandregulatesalternativepremrnaprocessing AT annkristinostlundfarrants swisnfassociateswithnascentpremrnpsandregulatesalternativepremrnaprocessing AT neusvisa swisnfassociateswithnascentpremrnpsandregulatesalternativepremrnaprocessing
_version_	1718414385690443776

SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.

Ejemplares similares