Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice

Ruminant-origin food products are the richest source of natural conjugated linoleic acid (CLA) in human diets, and cis-9, trans-11 (c9, t11) is the most abundant isomer of CLA. Butyrivibrio fibrisolvens, a rumen microbe, is considered responsible to have the highest CLA-producing potential. The pres...

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Autores principales:	Anima Srivastava, Sachin Kumar, Ashish Tyagi, Neeraj Shrivastava, Ajit Varma, Amrish Kumar Tyagi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Conjugated linoleic acid Butyrivibrio fibrisolvens Linoleic acid Probiotics Mice Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1b7c88c760c547469462b9b0460c53e3
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spelling	oai:doaj.org-article:1b7c88c760c547469462b9b0460c53e32021-12-02T05:03:39ZButyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice2590-262810.1016/j.crbiot.2021.11.001https://doaj.org/article/1b7c88c760c547469462b9b0460c53e32021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590262821000319https://doaj.org/toc/2590-2628Ruminant-origin food products are the richest source of natural conjugated linoleic acid (CLA) in human diets, and cis-9, trans-11 (c9, t11) is the most abundant isomer of CLA. Butyrivibrio fibrisolvens, a rumen microbe, is considered responsible to have the highest CLA-producing potential. The present study was carried out to identify and characterize a rumen-origin B. fibrisolvens strain that can produce high levels of c9, t11 CLA, as well as to evaluate how dietary supplementation with the strain influenced c9, t11 CLA levels in the gut and adipose tissue in mice. A total of 257 anaerobic strains were isolated from buffalo, cattle, and goat rumen samples, and 24 isolates were identified as Butyrivibrio spp. based on 16S rRNA sequencing. 22 isolates of Butyrivibrio spp. were found to be B. fibrisolven-like. These strains were tested for their potential to produce CLA at varied concentrations (0, 100, 200, 300, 400, 500 μg/mL) of linoleic acid (LA) as substrate after incubating for different durations (0, 2, 4, 6, 12, 24 and 36 h). Results of the study reflected that Butyrivibrio fibrisolvens F7 converted almost 74% of LA of 200 μg/mL concentration to c9, t11 CLA after 24 h and formed the maximum CLA (147.39 μg/mL). B. fibrisolvens F7 (GenBank Accession No: KU507371) was found to be acid and bile tolerant and exhibited good cell surface hydrophobicity in n-hexadecane and toluene. B. fibrisolvens F7 dietary supplementation in mice, combined with a high-LA diet, increases c9, t11 CLA levels in adipose tissue and gut by more than three folds. The results of this study significantly increase the possibility of employing B. fibrisolvens F7 as a probiotic supplement for animals to maintain a steady supply of natural c9, t11 CLA in the host.Anima SrivastavaSachin KumarAshish TyagiNeeraj ShrivastavaAjit VarmaAmrish Kumar TyagiElsevierarticleConjugated linoleic acidButyrivibrio fibrisolvensLinoleic acidProbioticsMiceBiotechnologyTP248.13-248.65ENCurrent Research in Biotechnology, Vol 3, Iss , Pp 300-307 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Conjugated linoleic acid Butyrivibrio fibrisolvens Linoleic acid Probiotics Mice Biotechnology TP248.13-248.65
spellingShingle	Conjugated linoleic acid Butyrivibrio fibrisolvens Linoleic acid Probiotics Mice Biotechnology TP248.13-248.65 Anima Srivastava Sachin Kumar Ashish Tyagi Neeraj Shrivastava Ajit Varma Amrish Kumar Tyagi Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice
description	Ruminant-origin food products are the richest source of natural conjugated linoleic acid (CLA) in human diets, and cis-9, trans-11 (c9, t11) is the most abundant isomer of CLA. Butyrivibrio fibrisolvens, a rumen microbe, is considered responsible to have the highest CLA-producing potential. The present study was carried out to identify and characterize a rumen-origin B. fibrisolvens strain that can produce high levels of c9, t11 CLA, as well as to evaluate how dietary supplementation with the strain influenced c9, t11 CLA levels in the gut and adipose tissue in mice. A total of 257 anaerobic strains were isolated from buffalo, cattle, and goat rumen samples, and 24 isolates were identified as Butyrivibrio spp. based on 16S rRNA sequencing. 22 isolates of Butyrivibrio spp. were found to be B. fibrisolven-like. These strains were tested for their potential to produce CLA at varied concentrations (0, 100, 200, 300, 400, 500 μg/mL) of linoleic acid (LA) as substrate after incubating for different durations (0, 2, 4, 6, 12, 24 and 36 h). Results of the study reflected that Butyrivibrio fibrisolvens F7 converted almost 74% of LA of 200 μg/mL concentration to c9, t11 CLA after 24 h and formed the maximum CLA (147.39 μg/mL). B. fibrisolvens F7 (GenBank Accession No: KU507371) was found to be acid and bile tolerant and exhibited good cell surface hydrophobicity in n-hexadecane and toluene. B. fibrisolvens F7 dietary supplementation in mice, combined with a high-LA diet, increases c9, t11 CLA levels in adipose tissue and gut by more than three folds. The results of this study significantly increase the possibility of employing B. fibrisolvens F7 as a probiotic supplement for animals to maintain a steady supply of natural c9, t11 CLA in the host.
format	article
author	Anima Srivastava Sachin Kumar Ashish Tyagi Neeraj Shrivastava Ajit Varma Amrish Kumar Tyagi
author_facet	Anima Srivastava Sachin Kumar Ashish Tyagi Neeraj Shrivastava Ajit Varma Amrish Kumar Tyagi
author_sort	Anima Srivastava
title	Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice
title_short	Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice
title_full	Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice
title_fullStr	Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice
title_full_unstemmed	Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice
title_sort	butyrivibrio fibrisolvens f7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1b7c88c760c547469462b9b0460c53e3
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Butyrivibrio fibrisolvens F7 dietary supplementation increases levels of cis 9-trans 11 conjugated linoleic acid in gut and adipose tissue in mice

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