Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma

Objective To observe the clinical significance of miR-135b-5p in oral squamous cell carcinoma (OSCC) tissues and to conduct a bioinformatics analysis of its predicted target genes. Methods The expression levels of miR-135b-5p in OSCC tissues and adjacent normal tissues were compared using data from...

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Autores principales:	ZHAO Ge, LI Changxue, GUO Chao, ZHU Hui
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2022
Materias:	oral squamous cell carcinoma microrna mir-135b-5p bioinformatics analysis the cancer genome atlas gene expression omnibus target gene calcium signaling pathway tumor molecular marker Medicine R
Acceso en línea:	https://doaj.org/article/1b85abba24014ec09d56774e734e70cd
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spelling	oai:doaj.org-article:1b85abba24014ec09d56774e734e70cd2021-11-23T06:17:19ZExpression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma10.12016/j.issn.2096-1456.2022.01.0052096-1456https://doaj.org/article/1b85abba24014ec09d56774e734e70cd2022-01-01T00:00:00Zhttp://www.kqjbfz.com/article/2022/2096-1456/2096-1456-30-1-27.shtmlhttps://doaj.org/toc/2096-1456Objective To observe the clinical significance of miR-135b-5p in oral squamous cell carcinoma (OSCC) tissues and to conduct a bioinformatics analysis of its predicted target genes. Methods The expression levels of miR-135b-5p in OSCC tissues and adjacent normal tissues were compared using data from TCGA and GEO databases, and the correlations of miR-135b-5p expression level with clinicopathologic characteristics were analyzed. Fresh tissues were collected in the clinic, and the expression of miR-135b-5p was verified by quantitative real-time PCR. The target genes with enriched pathways were analyzed by using bioinformatics methods. A protein-protein interaction network was constructed to screen hub genes. Results The expression levels of miR-135b-5p were significantly upregulated in OSCC tissues compared to adjacent normal tissues (P < 0.001) and had a good diagnostic capability (AUC=0.960, P < 0.001). The expression level of miR-135b-5p was positively correlated with histopathological grading (P=0.011). Enrichment analyses revealed that the target genes of miR-135b-5p were significantly associated with tumor-related signaling pathways, such as the calcium signaling pathway, the cGMP-PKG signaling pathway and the cAMP signaling pathway. Ten core target genes were obtained by screening: DLG2, ANK3, ERBB4, SCN2B, NBEA, GABRB2, ATP2B2, SNTA1, CACNA1D, and SPTBN4. Conclusion miR-135b-5p may act as an oncogene miRNA in OSCC and has the potential value of acting as a diagnostic biomarker and therapeutic target for OSCC.ZHAO GeLI ChangxueGUO ChaoZHU HuiEditorial Department of Journal of Prevention and Treatment for Stomatological Diseasesarticleoral squamous cell carcinomamicrornamir-135b-5pbioinformatics analysisthe cancer genome atlasgene expression omnibustarget genecalcium signaling pathwaytumor molecular markerMedicineRZH口腔疾病防治, Vol 30, Iss 1, Pp 27-32 (2022)
institution	DOAJ
collection	DOAJ
language	ZH
topic	oral squamous cell carcinoma microrna mir-135b-5p bioinformatics analysis the cancer genome atlas gene expression omnibus target gene calcium signaling pathway tumor molecular marker Medicine R
spellingShingle	oral squamous cell carcinoma microrna mir-135b-5p bioinformatics analysis the cancer genome atlas gene expression omnibus target gene calcium signaling pathway tumor molecular marker Medicine R ZHAO Ge LI Changxue GUO Chao ZHU Hui Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma
description	Objective To observe the clinical significance of miR-135b-5p in oral squamous cell carcinoma (OSCC) tissues and to conduct a bioinformatics analysis of its predicted target genes. Methods The expression levels of miR-135b-5p in OSCC tissues and adjacent normal tissues were compared using data from TCGA and GEO databases, and the correlations of miR-135b-5p expression level with clinicopathologic characteristics were analyzed. Fresh tissues were collected in the clinic, and the expression of miR-135b-5p was verified by quantitative real-time PCR. The target genes with enriched pathways were analyzed by using bioinformatics methods. A protein-protein interaction network was constructed to screen hub genes. Results The expression levels of miR-135b-5p were significantly upregulated in OSCC tissues compared to adjacent normal tissues (P < 0.001) and had a good diagnostic capability (AUC=0.960, P < 0.001). The expression level of miR-135b-5p was positively correlated with histopathological grading (P=0.011). Enrichment analyses revealed that the target genes of miR-135b-5p were significantly associated with tumor-related signaling pathways, such as the calcium signaling pathway, the cGMP-PKG signaling pathway and the cAMP signaling pathway. Ten core target genes were obtained by screening: DLG2, ANK3, ERBB4, SCN2B, NBEA, GABRB2, ATP2B2, SNTA1, CACNA1D, and SPTBN4. Conclusion miR-135b-5p may act as an oncogene miRNA in OSCC and has the potential value of acting as a diagnostic biomarker and therapeutic target for OSCC.
format	article
author	ZHAO Ge LI Changxue GUO Chao ZHU Hui
author_facet	ZHAO Ge LI Changxue GUO Chao ZHU Hui
author_sort	ZHAO Ge
title	Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma
title_short	Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma
title_full	Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma
title_fullStr	Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma
title_full_unstemmed	Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma
title_sort	expression and relevant bioinformatics analysis of mir-135b-5p in oral squamous cell carcinoma
publisher	Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
publishDate	2022
url	https://doaj.org/article/1b85abba24014ec09d56774e734e70cd
work_keys_str_mv	AT zhaoge expressionandrelevantbioinformaticsanalysisofmir135b5pinoralsquamouscellcarcinoma AT lichangxue expressionandrelevantbioinformaticsanalysisofmir135b5pinoralsquamouscellcarcinoma AT guochao expressionandrelevantbioinformaticsanalysisofmir135b5pinoralsquamouscellcarcinoma AT zhuhui expressionandrelevantbioinformaticsanalysisofmir135b5pinoralsquamouscellcarcinoma
_version_	1718416868872552448

Expression and relevant bioinformatics analysis of miR-135b-5p in oral squamous cell carcinoma

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