Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses

Abstract At the moment, cataract, which is the opacification of the eye’s lens, can only be treated by surgery. In order to develop and test new pharmacological treatment strategies for the disease, there is a need for an appropriate in vitro model using ex vivo animal lenses. In this study, porcine...

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Autores principales:	Manuel Ruiss, Martin Kronschläger, Andreas Schlatter, Thomas Dechat, Oliver Findl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b87215b956f482197e15195553cc332
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spelling	oai:doaj.org-article:1b87215b956f482197e15195553cc3322021-12-05T12:13:23ZComparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses10.1038/s41598-021-02851-62045-2322https://doaj.org/article/1b87215b956f482197e15195553cc3322021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02851-6https://doaj.org/toc/2045-2322Abstract At the moment, cataract, which is the opacification of the eye’s lens, can only be treated by surgery. In order to develop and test new pharmacological treatment strategies for the disease, there is a need for an appropriate in vitro model using ex vivo animal lenses. In this study, porcine lenses were incubated in either culture medium, glucose, triamcinolone acetonide, sodium chloride, hydrogen peroxide, sodium selenite, neutral buffered formalin, or were exposed to microwave heating to experimentally induce lens opacification. Changes in the lens morphology, weight, size, and elasticity were monitored 7 days after treatment. The fastest induction of dense opacification was seen in lenses exposed to sodium chloride, neutral buffered formalin, and microwave heating. No change in the size and weight of the lenses were detected, whereas loss in elasticity could be detected in lenses treated with formalin solution or microwave heating. Thus, neutral buffered formalin- and microwave-treated ex vivo porcine lenses seem to be a suitable model for mature cataracts, whereas hypertonic sodium chloride may be useful for studies on osmolarity-induced lens opacification.Manuel RuissMartin KronschlägerAndreas SchlatterThomas DechatOliver FindlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuel Ruiss Martin Kronschläger Andreas Schlatter Thomas Dechat Oliver Findl Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses
description	Abstract At the moment, cataract, which is the opacification of the eye’s lens, can only be treated by surgery. In order to develop and test new pharmacological treatment strategies for the disease, there is a need for an appropriate in vitro model using ex vivo animal lenses. In this study, porcine lenses were incubated in either culture medium, glucose, triamcinolone acetonide, sodium chloride, hydrogen peroxide, sodium selenite, neutral buffered formalin, or were exposed to microwave heating to experimentally induce lens opacification. Changes in the lens morphology, weight, size, and elasticity were monitored 7 days after treatment. The fastest induction of dense opacification was seen in lenses exposed to sodium chloride, neutral buffered formalin, and microwave heating. No change in the size and weight of the lenses were detected, whereas loss in elasticity could be detected in lenses treated with formalin solution or microwave heating. Thus, neutral buffered formalin- and microwave-treated ex vivo porcine lenses seem to be a suitable model for mature cataracts, whereas hypertonic sodium chloride may be useful for studies on osmolarity-induced lens opacification.
format	article
author	Manuel Ruiss Martin Kronschläger Andreas Schlatter Thomas Dechat Oliver Findl
author_facet	Manuel Ruiss Martin Kronschläger Andreas Schlatter Thomas Dechat Oliver Findl
author_sort	Manuel Ruiss
title	Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses
title_short	Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses
title_full	Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses
title_fullStr	Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses
title_full_unstemmed	Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses
title_sort	comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1b87215b956f482197e15195553cc332
work_keys_str_mv	AT manuelruiss comparisonofmethodstoexperimentallyinduceopacificationandelasticitychangeinexvivoporcinelenses AT martinkronschlager comparisonofmethodstoexperimentallyinduceopacificationandelasticitychangeinexvivoporcinelenses AT andreasschlatter comparisonofmethodstoexperimentallyinduceopacificationandelasticitychangeinexvivoporcinelenses AT thomasdechat comparisonofmethodstoexperimentallyinduceopacificationandelasticitychangeinexvivoporcinelenses AT oliverfindl comparisonofmethodstoexperimentallyinduceopacificationandelasticitychangeinexvivoporcinelenses
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Comparison of methods to experimentally induce opacification and elasticity change in ex vivo porcine lenses

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