Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

Burkholderia cepacia is an opportunistic pathogen that infects patients with debilitating underlying diseases. This study investigated the production of outer membrane vesicles (OMVs) by B. cepacia cultured with sub-minimum inhibitory concentrations (MICs) of antibiotics and examined their pathogeni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Se Yeon Kim, Mi Hyun Kim, Joo Hee Son, Seung Il Kim, Sung Ho Yun, Kyeongmin Kim, Shukho Kim, Minsang Shin, Je Chul Lee
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	burkholderia cepacia antibiotics outer membrane vesicle cytotoxicity inflammatory response Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/1b9073f84fcf45e48bc1f7bdd1e831ac
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b9073f84fcf45e48bc1f7bdd1e831ac
record_format	dspace
spelling	oai:doaj.org-article:1b9073f84fcf45e48bc1f7bdd1e831ac2021-11-17T14:21:58ZOuter membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses2150-55942150-560810.1080/21505594.2020.1802193https://doaj.org/article/1b9073f84fcf45e48bc1f7bdd1e831ac2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1802193https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Burkholderia cepacia is an opportunistic pathogen that infects patients with debilitating underlying diseases. This study investigated the production of outer membrane vesicles (OMVs) by B. cepacia cultured with sub-minimum inhibitory concentrations (MICs) of antibiotics and examined their pathogenic roles both in vitro and in vivo. B. cepacia ATCC 25416 produced more OMVs under antibiotic stress conditions than controls. OMVs isolated from B. cepacia cultured in Luria-Bertani (LB) broth (OMVs/LB) induced cytotoxicity and the expression of pro-inflammatory cytokine genes in A549 cells in a dose-dependent manner. Host cell cytotoxicity and pro-inflammatory responses were significantly higher in A549 cells treated with B. cepacia OMVs cultured with 1/4 MIC of ceftazidime (OMVs/CAZ) than in the cells treated with OMVs/LB, OMVs cultured with 1/4 MIC of trimethoprim/sulfamethoxazole (OMVs/SXT), or OMVs cultured with 1/4 MIC of meropenem. Intratracheal injection of B. cepacia OMVs also induced histopathology in vivo in mouse lungs. Expressions of IL-1β and TNF-α genes were significantly up-regulatedin the lungs of mice treated with OMVs/CAZ compared to mice administered other OMVs; the expression of the GRO-α gene, however, was significantly up-regulated in OMVs/SXT. In conclusion, OMVs produced by B. cepacia under different antibiotic stress conditions induce different host responses that may contribute to the pathogenesis of B. cepacia.Se Yeon KimMi Hyun KimJoo Hee SonSeung Il KimSung Ho YunKyeongmin KimShukho KimMinsang ShinJe Chul LeeTaylor & Francis Grouparticleburkholderia cepaciaantibioticsouter membrane vesiclecytotoxicityinflammatory responseInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 995-1005 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	burkholderia cepacia antibiotics outer membrane vesicle cytotoxicity inflammatory response Infectious and parasitic diseases RC109-216
spellingShingle	burkholderia cepacia antibiotics outer membrane vesicle cytotoxicity inflammatory response Infectious and parasitic diseases RC109-216 Se Yeon Kim Mi Hyun Kim Joo Hee Son Seung Il Kim Sung Ho Yun Kyeongmin Kim Shukho Kim Minsang Shin Je Chul Lee Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
description	Burkholderia cepacia is an opportunistic pathogen that infects patients with debilitating underlying diseases. This study investigated the production of outer membrane vesicles (OMVs) by B. cepacia cultured with sub-minimum inhibitory concentrations (MICs) of antibiotics and examined their pathogenic roles both in vitro and in vivo. B. cepacia ATCC 25416 produced more OMVs under antibiotic stress conditions than controls. OMVs isolated from B. cepacia cultured in Luria-Bertani (LB) broth (OMVs/LB) induced cytotoxicity and the expression of pro-inflammatory cytokine genes in A549 cells in a dose-dependent manner. Host cell cytotoxicity and pro-inflammatory responses were significantly higher in A549 cells treated with B. cepacia OMVs cultured with 1/4 MIC of ceftazidime (OMVs/CAZ) than in the cells treated with OMVs/LB, OMVs cultured with 1/4 MIC of trimethoprim/sulfamethoxazole (OMVs/SXT), or OMVs cultured with 1/4 MIC of meropenem. Intratracheal injection of B. cepacia OMVs also induced histopathology in vivo in mouse lungs. Expressions of IL-1β and TNF-α genes were significantly up-regulatedin the lungs of mice treated with OMVs/CAZ compared to mice administered other OMVs; the expression of the GRO-α gene, however, was significantly up-regulated in OMVs/SXT. In conclusion, OMVs produced by B. cepacia under different antibiotic stress conditions induce different host responses that may contribute to the pathogenesis of B. cepacia.
format	article
author	Se Yeon Kim Mi Hyun Kim Joo Hee Son Seung Il Kim Sung Ho Yun Kyeongmin Kim Shukho Kim Minsang Shin Je Chul Lee
author_facet	Se Yeon Kim Mi Hyun Kim Joo Hee Son Seung Il Kim Sung Ho Yun Kyeongmin Kim Shukho Kim Minsang Shin Je Chul Lee
author_sort	Se Yeon Kim
title	Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
title_short	Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
title_full	Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
title_fullStr	Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
title_full_unstemmed	Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
title_sort	outer membrane vesicles produced by burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/1b9073f84fcf45e48bc1f7bdd1e831ac
work_keys_str_mv	AT seyeonkim outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT mihyunkim outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT jooheeson outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT seungilkim outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT sunghoyun outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT kyeongminkim outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT shukhokim outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT minsangshin outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses AT jechullee outermembranevesiclesproducedbyburkholderiacepaciaculturedwithsubinhibitoryconcentrationsofceftazidimeenhanceproinflammatoryresponses
_version_	1718425466854965248

Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

Ejemplares similares