RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE

We studied the effect of a recombinant murine TNF (rmTNF) and TNF-binding protein of Variola virus (VARV-CrmB) upon colony-forming ability of bone marrow cells (BMC) from Balb/c mice. BMC were grown in semisolid methylcellulose medium supplemented with murine growth factors in the absence or presenc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	L. B. Toporkova, A. A. Petuhova, I. P. Gileva, I. A. Orlovskaya
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	erythroid precursors granulocyte-macrophage precursors tnf tnf-binding protein Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1b95e25546ea4e2bae5fd5b7067dbe7b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b95e25546ea4e2bae5fd5b7067dbe7b
record_format	dspace
spelling	oai:doaj.org-article:1b95e25546ea4e2bae5fd5b7067dbe7b2021-11-18T08:03:38ZRECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE1563-06252313-741X10.15789/1563-0625-2010-4-5-297-304https://doaj.org/article/1b95e25546ea4e2bae5fd5b7067dbe7b2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/128https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWe studied the effect of a recombinant murine TNF (rmTNF) and TNF-binding protein of Variola virus (VARV-CrmB) upon colony-forming ability of bone marrow cells (BMC) from Balb/c mice. BMC were grown in semisolid methylcellulose medium supplemented with murine growth factors in the absence or presence of mrTNF along at concentrations ranging from 2 to 40 ng/ml, VARV-CrmB (2 to 24 ng/ml), as well as in presence of both mrTNF (2 ng/ml) and VARV-CrmB (2 to 12 ng/ml). Hematopoietic colonies (BFU-E, CFU-E, and CFU-GM) were scored on day 14. VARV-CrmB protein didn't influence clonogenicity of the bone marrow progenitors. rmTNF inhibited growth of erythroid cells (BFU-E+CFU-E) at all concentrations tested, and stimulated growth of granulocyte-macrophage progenitors (CFU-GM) at concentrations of 2 ng/ml and 10 ng/ml. Combined effect of rmTNF and VARV-CrmB resulted into abolition of rmTNF-induced reduction of BFU-E+CFU-E formation and rmTNF-induced increase of CFU-GM number up to basic levels. Thеse results clearly demonstrate the anti-TNF activity of recombinant viral VARV-CrmB protein.L. B. ToporkovaA. A. PetuhovaI. P. GilevaI. A. OrlovskayaSPb RAACIarticleerythroid precursorsgranulocyte-macrophage precursorstnftnf-binding proteinImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 4-5, Pp 297-304 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	erythroid precursors granulocyte-macrophage precursors tnf tnf-binding protein Immunologic diseases. Allergy RC581-607
spellingShingle	erythroid precursors granulocyte-macrophage precursors tnf tnf-binding protein Immunologic diseases. Allergy RC581-607 L. B. Toporkova A. A. Petuhova I. P. Gileva I. A. Orlovskaya RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE
description	We studied the effect of a recombinant murine TNF (rmTNF) and TNF-binding protein of Variola virus (VARV-CrmB) upon colony-forming ability of bone marrow cells (BMC) from Balb/c mice. BMC were grown in semisolid methylcellulose medium supplemented with murine growth factors in the absence or presence of mrTNF along at concentrations ranging from 2 to 40 ng/ml, VARV-CrmB (2 to 24 ng/ml), as well as in presence of both mrTNF (2 ng/ml) and VARV-CrmB (2 to 12 ng/ml). Hematopoietic colonies (BFU-E, CFU-E, and CFU-GM) were scored on day 14. VARV-CrmB protein didn't influence clonogenicity of the bone marrow progenitors. rmTNF inhibited growth of erythroid cells (BFU-E+CFU-E) at all concentrations tested, and stimulated growth of granulocyte-macrophage progenitors (CFU-GM) at concentrations of 2 ng/ml and 10 ng/ml. Combined effect of rmTNF and VARV-CrmB resulted into abolition of rmTNF-induced reduction of BFU-E+CFU-E formation and rmTNF-induced increase of CFU-GM number up to basic levels. Thеse results clearly demonstrate the anti-TNF activity of recombinant viral VARV-CrmB protein.
format	article
author	L. B. Toporkova A. A. Petuhova I. P. Gileva I. A. Orlovskaya
author_facet	L. B. Toporkova A. A. Petuhova I. P. Gileva I. A. Orlovskaya
author_sort	L. B. Toporkova
title	RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE
title_short	RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE
title_full	RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE
title_fullStr	RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE
title_full_unstemmed	RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE
title_sort	recombinant protein of variola virus abolishes the effects of tumor necrosis factor upon marrow hematopoiesis in balb/с mice
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/1b95e25546ea4e2bae5fd5b7067dbe7b
work_keys_str_mv	AT lbtoporkova recombinantproteinofvariolavirusabolishestheeffectsoftumornecrosisfactoruponmarrowhematopoiesisinbalbsmice AT aapetuhova recombinantproteinofvariolavirusabolishestheeffectsoftumornecrosisfactoruponmarrowhematopoiesisinbalbsmice AT ipgileva recombinantproteinofvariolavirusabolishestheeffectsoftumornecrosisfactoruponmarrowhematopoiesisinbalbsmice AT iaorlovskaya recombinantproteinofvariolavirusabolishestheeffectsoftumornecrosisfactoruponmarrowhematopoiesisinbalbsmice
_version_	1718422528424148992

RECOMBINANT PROTEIN OF VARIOLA VIRUS ABOLISHES THE EFFECTS OF TUMOR NECROSIS FACTOR UPON MARROW HEMATOPOIESIS IN BALB/С MICE

Ejemplares similares