Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages

During definitive erythropoiesis, maturation of erythroid progenitors into enucleated reticulocytes requires the erythroblastic island (EBI) niche comprising a central macrophage attached to differentiating erythroid progenitors. Normally, the macrophage provides a nurturing environment for maturati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kaustav Mukherjee, James J. Bieker
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	erythroblastic island mammalian hematopoiesis macrophage identity erythropoiesis transcriptional control single cell analyses Genetics QH426-470
Acceso en línea:	https://doaj.org/article/1b9afa530f5b42edb1bced44db6697bd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b9afa530f5b42edb1bced44db6697bd
record_format	dspace
spelling	oai:doaj.org-article:1b9afa530f5b42edb1bced44db6697bd2021-11-22T07:05:04ZTranscriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages1664-802110.3389/fgene.2021.756028https://doaj.org/article/1b9afa530f5b42edb1bced44db6697bd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.756028/fullhttps://doaj.org/toc/1664-8021During definitive erythropoiesis, maturation of erythroid progenitors into enucleated reticulocytes requires the erythroblastic island (EBI) niche comprising a central macrophage attached to differentiating erythroid progenitors. Normally, the macrophage provides a nurturing environment for maturation of erythroid cells. Its critical physiologic importance entails aiding in recovery from anemic insults, such as systemic stress or acquired disease. Considerable interest in characterizing the central macrophage of the island niche led to the identification of putative cell surface markers enriched in island macrophages, enabling isolation and characterization. Recent studies focus on bulk and single cell transcriptomics of the island macrophage during adult steady-state erythropoiesis and embryonic erythropoiesis. They reveal that the island macrophage is a distinct cell type but with widespread cellular heterogeneity, likely suggesting distinct developmental origins and biological function. These studies have also uncovered transcriptional programs that drive gene expression in the island macrophage. Strikingly, the master erythroid regulator EKLF/Klf1 seems to also play a major role in specifying gene expression in island macrophages, including a putative EKLF/Klf1-dependent transcription circuit. Our present review and analysis of mouse single cell genetic patterns suggest novel expression characteristics that will enable a clear enrichment of EBI subtypes and resolution of island macrophage heterogeneity. Specifically, the discovery of markers such as Epor, and specific features for EKLF/Klf1-expressing island macrophages such as Sptb and Add2, or for SpiC-expressing island macrophage such as Timd4, or for Maf/Nr1h3-expressing island macrophage such as Vcam1, opens exciting possibilities for further characterization of these unique macrophage cell types in the context of their critical developmental function.Kaustav MukherjeeKaustav MukherjeeJames J. BiekerJames J. BiekerJames J. BiekerJames J. BiekerFrontiers Media S.A.articleerythroblastic islandmammalian hematopoiesismacrophage identityerythropoiesistranscriptional controlsingle cell analysesGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	erythroblastic island mammalian hematopoiesis macrophage identity erythropoiesis transcriptional control single cell analyses Genetics QH426-470
spellingShingle	erythroblastic island mammalian hematopoiesis macrophage identity erythropoiesis transcriptional control single cell analyses Genetics QH426-470 Kaustav Mukherjee Kaustav Mukherjee James J. Bieker James J. Bieker James J. Bieker James J. Bieker Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages
description	During definitive erythropoiesis, maturation of erythroid progenitors into enucleated reticulocytes requires the erythroblastic island (EBI) niche comprising a central macrophage attached to differentiating erythroid progenitors. Normally, the macrophage provides a nurturing environment for maturation of erythroid cells. Its critical physiologic importance entails aiding in recovery from anemic insults, such as systemic stress or acquired disease. Considerable interest in characterizing the central macrophage of the island niche led to the identification of putative cell surface markers enriched in island macrophages, enabling isolation and characterization. Recent studies focus on bulk and single cell transcriptomics of the island macrophage during adult steady-state erythropoiesis and embryonic erythropoiesis. They reveal that the island macrophage is a distinct cell type but with widespread cellular heterogeneity, likely suggesting distinct developmental origins and biological function. These studies have also uncovered transcriptional programs that drive gene expression in the island macrophage. Strikingly, the master erythroid regulator EKLF/Klf1 seems to also play a major role in specifying gene expression in island macrophages, including a putative EKLF/Klf1-dependent transcription circuit. Our present review and analysis of mouse single cell genetic patterns suggest novel expression characteristics that will enable a clear enrichment of EBI subtypes and resolution of island macrophage heterogeneity. Specifically, the discovery of markers such as Epor, and specific features for EKLF/Klf1-expressing island macrophages such as Sptb and Add2, or for SpiC-expressing island macrophage such as Timd4, or for Maf/Nr1h3-expressing island macrophage such as Vcam1, opens exciting possibilities for further characterization of these unique macrophage cell types in the context of their critical developmental function.
format	article
author	Kaustav Mukherjee Kaustav Mukherjee James J. Bieker James J. Bieker James J. Bieker James J. Bieker
author_facet	Kaustav Mukherjee Kaustav Mukherjee James J. Bieker James J. Bieker James J. Bieker James J. Bieker
author_sort	Kaustav Mukherjee
title	Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages
title_short	Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages
title_full	Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages
title_fullStr	Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages
title_full_unstemmed	Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages
title_sort	transcriptional control of gene expression and the heterogeneous cellular identity of erythroblastic island macrophages
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1b9afa530f5b42edb1bced44db6697bd
work_keys_str_mv	AT kaustavmukherjee transcriptionalcontrolofgeneexpressionandtheheterogeneouscellularidentityoferythroblasticislandmacrophages AT kaustavmukherjee transcriptionalcontrolofgeneexpressionandtheheterogeneouscellularidentityoferythroblasticislandmacrophages AT jamesjbieker transcriptionalcontrolofgeneexpressionandtheheterogeneouscellularidentityoferythroblasticislandmacrophages AT jamesjbieker transcriptionalcontrolofgeneexpressionandtheheterogeneouscellularidentityoferythroblasticislandmacrophages AT jamesjbieker transcriptionalcontrolofgeneexpressionandtheheterogeneouscellularidentityoferythroblasticislandmacrophages AT jamesjbieker transcriptionalcontrolofgeneexpressionandtheheterogeneouscellularidentityoferythroblasticislandmacrophages
_version_	1718418088687304704

Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages

Ejemplares similares