Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions

Abstract SARS-CoV-2 is an enveloped virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. Here, single viruses were analyzed by atomic force microscopy (AFM) operating directly in a level 3 biosafety (BSL3) facility, which appeared as a fast and powerful method to assess at the nan...

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Autores principales:	Sébastien Lyonnais, Mathilde Hénaut, Aymeric Neyret, Peggy Merida, Chantal Cazevieille, Nathalie Gros, Christine Chable-Bessia, Delphine Muriaux
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1baa872d667f436482764f75e9760d45
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spelling	oai:doaj.org-article:1baa872d667f436482764f75e9760d452021-12-02T17:51:22ZAtomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions10.1038/s41598-021-91371-42045-2322https://doaj.org/article/1baa872d667f436482764f75e9760d452021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91371-4https://doaj.org/toc/2045-2322Abstract SARS-CoV-2 is an enveloped virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. Here, single viruses were analyzed by atomic force microscopy (AFM) operating directly in a level 3 biosafety (BSL3) facility, which appeared as a fast and powerful method to assess at the nanoscale level and in 3D infectious virus morphology in its native conformation, or upon inactivation treatments. AFM imaging reveals structurally intact infectious and inactivated SARS-CoV-2 upon low concentration of formaldehyde treatment. This protocol combining AFM and plaque assays allows the preparation of intact inactivated SARS-CoV-2 particles for safe use of samples out of level 3 laboratory to accelerate researches against the COVID-19 pandemic. Overall, we illustrate how adapted BSL3-AFM is a remarkable toolbox for rapid and direct virus analysis based on nanoscale morphology.Sébastien LyonnaisMathilde HénautAymeric NeyretPeggy MeridaChantal CazevieilleNathalie GrosChristine Chable-BessiaDelphine MuriauxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sébastien Lyonnais Mathilde Hénaut Aymeric Neyret Peggy Merida Chantal Cazevieille Nathalie Gros Christine Chable-Bessia Delphine Muriaux Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions
description	Abstract SARS-CoV-2 is an enveloped virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. Here, single viruses were analyzed by atomic force microscopy (AFM) operating directly in a level 3 biosafety (BSL3) facility, which appeared as a fast and powerful method to assess at the nanoscale level and in 3D infectious virus morphology in its native conformation, or upon inactivation treatments. AFM imaging reveals structurally intact infectious and inactivated SARS-CoV-2 upon low concentration of formaldehyde treatment. This protocol combining AFM and plaque assays allows the preparation of intact inactivated SARS-CoV-2 particles for safe use of samples out of level 3 laboratory to accelerate researches against the COVID-19 pandemic. Overall, we illustrate how adapted BSL3-AFM is a remarkable toolbox for rapid and direct virus analysis based on nanoscale morphology.
format	article
author	Sébastien Lyonnais Mathilde Hénaut Aymeric Neyret Peggy Merida Chantal Cazevieille Nathalie Gros Christine Chable-Bessia Delphine Muriaux
author_facet	Sébastien Lyonnais Mathilde Hénaut Aymeric Neyret Peggy Merida Chantal Cazevieille Nathalie Gros Christine Chable-Bessia Delphine Muriaux
author_sort	Sébastien Lyonnais
title	Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions
title_short	Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions
title_full	Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions
title_fullStr	Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions
title_full_unstemmed	Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions
title_sort	atomic force microscopy analysis of native infectious and inactivated sars-cov-2 virions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1baa872d667f436482764f75e9760d45
work_keys_str_mv	AT sebastienlyonnais atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions AT mathildehenaut atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions AT aymericneyret atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions AT peggymerida atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions AT chantalcazevieille atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions AT nathaliegros atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions AT christinechablebessia atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions AT delphinemuriaux atomicforcemicroscopyanalysisofnativeinfectiousandinactivatedsarscov2virions
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Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions

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