Overwintering honeybees maintained dynamic and stable intestinal bacteria

Abstract Honeybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored...

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Autores principales:	Peng Liu, Yujie Zhu, Liang Ye, Tengfei Shi, Lai Li, Haiqun Cao, Linsheng Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1bb0898163454cb084dba65938d9d3be
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spelling	oai:doaj.org-article:1bb0898163454cb084dba65938d9d3be2021-11-21T12:25:16ZOverwintering honeybees maintained dynamic and stable intestinal bacteria10.1038/s41598-021-01204-72045-2322https://doaj.org/article/1bb0898163454cb084dba65938d9d3be2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01204-7https://doaj.org/toc/2045-2322Abstract Honeybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored dynamic changes to the intestinal bacteria in overwintering honeybees. We collected bee samples before overwintering, during prophase of overwintering, metaphase of overwintering, anaphase of overwintering, telophase of overwintering, and after overwintering. By using high-throughput sequencing targeting the V3−V4 regions of the 16S rDNA, the abundance of the intestinal bacteria were analyzed in overwintering honeybees. A total of 1,373,886 high-quality sequences were acquired and Proteobacteria (85.69%), Firmicutes (10.40%), Actinobacteria (3.66%), and Cyanobacteria (1.87%) were identified as major components of the intestinal bacteria. All core honeybee intestinal bacteria genera, such as Gilliamella, Bartonella, Snodgrassella, Lactobacillus, Frischella, Commensalibacter, and Bifidobacterium were detected. The abundance of Actinobacteria, Bartonella, and Bifidobacterium increased initially and then decreased in winter honeybees. There were no significant differences in the richness and evenness of the microbiota in overwintering honeybees; however, there was a statistically significant difference in the beta diversity of the intestinal bacteria after overwintering compared with that in other groups. Our results suggested that honeybees maintained their intestinal ecosystem balance, and increased the abundance of gut probiotics in response to environmental and nutrition pressures in winter.Peng LiuYujie ZhuLiang YeTengfei ShiLai LiHaiqun CaoLinsheng YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Liu Yujie Zhu Liang Ye Tengfei Shi Lai Li Haiqun Cao Linsheng Yu Overwintering honeybees maintained dynamic and stable intestinal bacteria
description	Abstract Honeybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored dynamic changes to the intestinal bacteria in overwintering honeybees. We collected bee samples before overwintering, during prophase of overwintering, metaphase of overwintering, anaphase of overwintering, telophase of overwintering, and after overwintering. By using high-throughput sequencing targeting the V3−V4 regions of the 16S rDNA, the abundance of the intestinal bacteria were analyzed in overwintering honeybees. A total of 1,373,886 high-quality sequences were acquired and Proteobacteria (85.69%), Firmicutes (10.40%), Actinobacteria (3.66%), and Cyanobacteria (1.87%) were identified as major components of the intestinal bacteria. All core honeybee intestinal bacteria genera, such as Gilliamella, Bartonella, Snodgrassella, Lactobacillus, Frischella, Commensalibacter, and Bifidobacterium were detected. The abundance of Actinobacteria, Bartonella, and Bifidobacterium increased initially and then decreased in winter honeybees. There were no significant differences in the richness and evenness of the microbiota in overwintering honeybees; however, there was a statistically significant difference in the beta diversity of the intestinal bacteria after overwintering compared with that in other groups. Our results suggested that honeybees maintained their intestinal ecosystem balance, and increased the abundance of gut probiotics in response to environmental and nutrition pressures in winter.
format	article
author	Peng Liu Yujie Zhu Liang Ye Tengfei Shi Lai Li Haiqun Cao Linsheng Yu
author_facet	Peng Liu Yujie Zhu Liang Ye Tengfei Shi Lai Li Haiqun Cao Linsheng Yu
author_sort	Peng Liu
title	Overwintering honeybees maintained dynamic and stable intestinal bacteria
title_short	Overwintering honeybees maintained dynamic and stable intestinal bacteria
title_full	Overwintering honeybees maintained dynamic and stable intestinal bacteria
title_fullStr	Overwintering honeybees maintained dynamic and stable intestinal bacteria
title_full_unstemmed	Overwintering honeybees maintained dynamic and stable intestinal bacteria
title_sort	overwintering honeybees maintained dynamic and stable intestinal bacteria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1bb0898163454cb084dba65938d9d3be
work_keys_str_mv	AT pengliu overwinteringhoneybeesmaintaineddynamicandstableintestinalbacteria AT yujiezhu overwinteringhoneybeesmaintaineddynamicandstableintestinalbacteria AT liangye overwinteringhoneybeesmaintaineddynamicandstableintestinalbacteria AT tengfeishi overwinteringhoneybeesmaintaineddynamicandstableintestinalbacteria AT laili overwinteringhoneybeesmaintaineddynamicandstableintestinalbacteria AT haiquncao overwinteringhoneybeesmaintaineddynamicandstableintestinalbacteria AT linshengyu overwinteringhoneybeesmaintaineddynamicandstableintestinalbacteria
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Overwintering honeybees maintained dynamic and stable intestinal bacteria

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