Autophagy regulates the stemness of cervical cancer stem cells

Yi Yang,1,2 Li Yu,1 Jin Li,1 Ya Hong Yuan,1 Xiao Li Wang,1 Shi Rong Yan,1 Dong Sheng Li,1 Yan Ding1 1Hubei Key Laboratory of Embryonic Stem Cell Research, 2Reproductive Center, Taihe Hospital, Hubei University of Medicine, Shiyan, People’s Republic of China Abstract: Cancer stem cells (CSC...

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Autores principales:	Yang Y, Yu L, Li J, Yuan YH, Wang XL, Yan SR, Li DS, Ding Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	cervical cancer autophagy cancer stem cell Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1bb2052aad394a379e2626d36f09e22b
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spelling	oai:doaj.org-article:1bb2052aad394a379e2626d36f09e22b2021-12-02T00:15:32ZAutophagy regulates the stemness of cervical cancer stem cells1177-5491https://doaj.org/article/1bb2052aad394a379e2626d36f09e22b2017-06-01T00:00:00Zhttps://www.dovepress.com/autophagy-regulates-the-stemness-of-cervical-cancer-stem-cells-peer-reviewed-article-BTThttps://doaj.org/toc/1177-5491Yi Yang,1,2 Li Yu,1 Jin Li,1 Ya Hong Yuan,1 Xiao Li Wang,1 Shi Rong Yan,1 Dong Sheng Li,1 Yan Ding1 1Hubei Key Laboratory of Embryonic Stem Cell Research, 2Reproductive Center, Taihe Hospital, Hubei University of Medicine, Shiyan, People’s Republic of China Abstract: Cancer stem cells (CSCs) are a rare population of multipotent cells with the capacity to self-renew. It has been reported that there are CSCs in cervical cancer cells. Pluripotency-associated (PA) transcription factors such as Oct4, Sox2, Nanog and CD44 have been used to isolate CSCs subpopulations. In this study, we showed that autophagy plays an important role in the biological behavior of cervical cancer cells. The expression of the autophagy protein Beclin 1 and LC3B was higher in tumorspheres established from human cervical cancers cell lines (and CaSki) than in the parental adherent cells. It was also observed that the basal and starvation-induced autophagy ﬂux was higher in tumorspheres than in the bulk population. Autophagy could regulate the expression level of PA proteins in cervical CSCs. In addition, CRISPR/Cas 9-mediated Beclin 1 knockout enhanced the malignancy of HeLa cells, leading to accumulation of PA proteins and promoted tumorsphere formation. Our findings suggest that autophagy modulates homeostasis of PA proteins, and Beclin 1 is critical for CSC maintenance and tumor development in nude mice. This demonstrates that a prosurvival autophagic pathway is critical for CSC maintenance. Keywords: cervical cancer, autophagy, cancer stem cell, LC3, Oct4Yang YYu LLi JYuan YHWang XLYan SRLi DSDing YDove Medical Pressarticlecervical cancerautophagycancer stem cellMedicine (General)R5-920ENBiologics: Targets & Therapy, Vol Volume 11, Pp 71-79 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	cervical cancer autophagy cancer stem cell Medicine (General) R5-920
spellingShingle	cervical cancer autophagy cancer stem cell Medicine (General) R5-920 Yang Y Yu L Li J Yuan YH Wang XL Yan SR Li DS Ding Y Autophagy regulates the stemness of cervical cancer stem cells
description	Yi Yang,1,2 Li Yu,1 Jin Li,1 Ya Hong Yuan,1 Xiao Li Wang,1 Shi Rong Yan,1 Dong Sheng Li,1 Yan Ding1 1Hubei Key Laboratory of Embryonic Stem Cell Research, 2Reproductive Center, Taihe Hospital, Hubei University of Medicine, Shiyan, People’s Republic of China Abstract: Cancer stem cells (CSCs) are a rare population of multipotent cells with the capacity to self-renew. It has been reported that there are CSCs in cervical cancer cells. Pluripotency-associated (PA) transcription factors such as Oct4, Sox2, Nanog and CD44 have been used to isolate CSCs subpopulations. In this study, we showed that autophagy plays an important role in the biological behavior of cervical cancer cells. The expression of the autophagy protein Beclin 1 and LC3B was higher in tumorspheres established from human cervical cancers cell lines (and CaSki) than in the parental adherent cells. It was also observed that the basal and starvation-induced autophagy ﬂux was higher in tumorspheres than in the bulk population. Autophagy could regulate the expression level of PA proteins in cervical CSCs. In addition, CRISPR/Cas 9-mediated Beclin 1 knockout enhanced the malignancy of HeLa cells, leading to accumulation of PA proteins and promoted tumorsphere formation. Our findings suggest that autophagy modulates homeostasis of PA proteins, and Beclin 1 is critical for CSC maintenance and tumor development in nude mice. This demonstrates that a prosurvival autophagic pathway is critical for CSC maintenance. Keywords: cervical cancer, autophagy, cancer stem cell, LC3, Oct4
format	article
author	Yang Y Yu L Li J Yuan YH Wang XL Yan SR Li DS Ding Y
author_facet	Yang Y Yu L Li J Yuan YH Wang XL Yan SR Li DS Ding Y
author_sort	Yang Y
title	Autophagy regulates the stemness of cervical cancer stem cells
title_short	Autophagy regulates the stemness of cervical cancer stem cells
title_full	Autophagy regulates the stemness of cervical cancer stem cells
title_fullStr	Autophagy regulates the stemness of cervical cancer stem cells
title_full_unstemmed	Autophagy regulates the stemness of cervical cancer stem cells
title_sort	autophagy regulates the stemness of cervical cancer stem cells
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/1bb2052aad394a379e2626d36f09e22b
work_keys_str_mv	AT yangy autophagyregulatesthestemnessofcervicalcancerstemcells AT yul autophagyregulatesthestemnessofcervicalcancerstemcells AT lij autophagyregulatesthestemnessofcervicalcancerstemcells AT yuanyh autophagyregulatesthestemnessofcervicalcancerstemcells AT wangxl autophagyregulatesthestemnessofcervicalcancerstemcells AT yansr autophagyregulatesthestemnessofcervicalcancerstemcells AT lids autophagyregulatesthestemnessofcervicalcancerstemcells AT dingy autophagyregulatesthestemnessofcervicalcancerstemcells
_version_	1718403849460383744

Autophagy regulates the stemness of cervical cancer stem cells

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