Study on the mechanism of Wenyang Jieyu Granules in the treatment of depression based on network pharmacology
Objective: To explore the potential antidepressant mechanism of Wenyang Jieyu Granules with multiple targets and components based on network pharmacology, and to verify the cAMP pathway and animal experimental results. Methods: The active components of Aconitum, Guizhi, Glycyrrhiza uralensis, Wum...
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Editorial Board of Journal of Hainan Medical University
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/1bb27474cdf64e84985746b1c0fc4aab |
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| Sumario: | Objective: To explore the potential antidepressant mechanism of Wenyang Jieyu Granules
with multiple targets and components based on network pharmacology, and to verify the cAMP
pathway and animal experimental results. Methods: The active components of Aconitum,
Guizhi, Glycyrrhiza uralensis, Wumei, Jujube and Ginger in Wenyang Jieyu Granules were
searched and screened by TCM System Pharmacology Analysis Platform (TCMSP). Using
the Swiss database to predict its target, and then through the UniProt database to query the
target corresponding genes; with the use of Gene Cards、OMIM database query depression
related target Point and gene mapping to get the potential target of Wenyang Jieyu granule
antidepressant. using Cytoscape, String software to plot the "wenyangjieyu granule-active
component-potential action target-depression" network and "protein interaction network ", and
using David database for GO functional enrichment analysis and KEGG pathway enrichment
analysis to predict the action mechanism of wenyangjieyu granule in the treatment of
depression and verify it with some previous relevant experimental results. Autodock software
was used to perform molecular docking experiments on selected components and targets.
Results: The topology analysis is effective there are 47 core components of depression, and 40
potential key targets are SLC6A2, SHBG, ACHE, NR3C1, ESR1. they are involved in many
biological processes, such as neurotransmitter receptor conduction, ammonium ion binding,
postsynaptic neurotransmitter receptor activity, G protein-coupled neurotransmitter receptor
activity. they play an antidepressant effect through brain tissue nerve activity ligand-receptor
interaction signaling pathway, cAMP, PI3K-Akt, MAPK signaling pathway, ErbB signaling
pathway, etc. At the same time, the results of molecular docking show that the core components
have good binding force to the key targets. Conclusion: Wenyang Jieyu granule. The active
components of the anti-depressant effect are mediated by multiple signaling pathways and
multiple targets. |
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