Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona

Abstract Reconfiguration of protein conformation in a micro and nano particle (MNP) protein corona due to interaction is an often-overlooked aspect in drug design and nano-medicine. Mostly, MNP-Protein corona studies focus on the toxicity of nano particles (NPs) in a biological environment to analyz...

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Autores principales:	Urvashi Singh, Zeeshan Saifi, Mridul Kumar, Armin Reimers, Soami Daya Krishnananda, Rainer Adelung, Martina Baum
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1bc49620fde24c2d80288c1dc5bb36fe
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spelling	oai:doaj.org-article:1bc49620fde24c2d80288c1dc5bb36fe2021-12-02T16:35:46ZRole of structural specificity of ZnO particles in preserving functionality of proteins in their corona10.1038/s41598-021-95540-32045-2322https://doaj.org/article/1bc49620fde24c2d80288c1dc5bb36fe2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95540-3https://doaj.org/toc/2045-2322Abstract Reconfiguration of protein conformation in a micro and nano particle (MNP) protein corona due to interaction is an often-overlooked aspect in drug design and nano-medicine. Mostly, MNP-Protein corona studies focus on the toxicity of nano particles (NPs) in a biological environment to analyze biocompatibility. However, preserving functional specificity of proteins in an NP corona becomes critical for effective translation of nano-medicine. This paper investigates the non-classical interaction between insulin and ZnO MNPs using a classical electrical characterization technique at GHz frequency with an objective to understand the effect of the micro particle (MP) and nanoparticle (NP) morphology on the electrical characteristics of the MNP-Protein corona and therefore the conformation and functional specificity of protein. The MNP-Protein corona was subjected to thermal and enzymatic (papain) perturbation to study the denaturation of the protein. Experimental results demonstrate that the morphology of ZnO particles plays an important role in preserving the electrical characteristics of insulin.Urvashi SinghZeeshan SaifiMridul KumarArmin ReimersSoami Daya KrishnanandaRainer AdelungMartina BaumNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Urvashi Singh Zeeshan Saifi Mridul Kumar Armin Reimers Soami Daya Krishnananda Rainer Adelung Martina Baum Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona
description	Abstract Reconfiguration of protein conformation in a micro and nano particle (MNP) protein corona due to interaction is an often-overlooked aspect in drug design and nano-medicine. Mostly, MNP-Protein corona studies focus on the toxicity of nano particles (NPs) in a biological environment to analyze biocompatibility. However, preserving functional specificity of proteins in an NP corona becomes critical for effective translation of nano-medicine. This paper investigates the non-classical interaction between insulin and ZnO MNPs using a classical electrical characterization technique at GHz frequency with an objective to understand the effect of the micro particle (MP) and nanoparticle (NP) morphology on the electrical characteristics of the MNP-Protein corona and therefore the conformation and functional specificity of protein. The MNP-Protein corona was subjected to thermal and enzymatic (papain) perturbation to study the denaturation of the protein. Experimental results demonstrate that the morphology of ZnO particles plays an important role in preserving the electrical characteristics of insulin.
format	article
author	Urvashi Singh Zeeshan Saifi Mridul Kumar Armin Reimers Soami Daya Krishnananda Rainer Adelung Martina Baum
author_facet	Urvashi Singh Zeeshan Saifi Mridul Kumar Armin Reimers Soami Daya Krishnananda Rainer Adelung Martina Baum
author_sort	Urvashi Singh
title	Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona
title_short	Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona
title_full	Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona
title_fullStr	Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona
title_full_unstemmed	Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona
title_sort	role of structural specificity of zno particles in preserving functionality of proteins in their corona
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1bc49620fde24c2d80288c1dc5bb36fe
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Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona

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