Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Lei Wang1,2,*, Haijun Zhang1,2,*, Baoan Chen1,2, Guohua Xia1,2, Shuai Wang1,2, Jian Cheng1,2, Zeye Shao1,2, Chong Gao1,2, Wen Bao1,2, Liang Tian1,2, Yanyan Ren1,2, Peipei Xu1,2, Xiaohui Cai1,2, Ran Liu1,2, Xuemei Wang3 1Department of Hematology and Oncology, Zhongda Hospital, Medical School, 2Facult...

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Autores principales:	Wang XM, Liu R, Cai XH, Xu PP, Ren YY, Tian L, Bao W, Gao C, Shao Z, Cheng J, Wang S, Xia GH, Chen BA, Zhang HJ, Wang L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1bc7fd136bfa445aa0c593757bb25df9
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spelling	oai:doaj.org-article:1bc7fd136bfa445aa0c593757bb25df92021-12-02T07:28:37ZEffect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells1176-91141178-2013https://doaj.org/article/1bc7fd136bfa445aa0c593757bb25df92012-02-01T00:00:00Zhttp://www.dovepress.com/effect-of-magnetic-nanoparticles-on-apoptosis-and-cell-cycle-induced-b-a9265https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Lei Wang1,2,, Haijun Zhang1,2,, Baoan Chen1,2, Guohua Xia1,2, Shuai Wang1,2, Jian Cheng1,2, Zeye Shao1,2, Chong Gao1,2, Wen Bao1,2, Liang Tian1,2, Yanyan Ren1,2, Peipei Xu1,2, Xiaohui Cai1,2, Ran Liu1,2, Xuemei Wang3 1Department of Hematology and Oncology, Zhongda Hospital, Medical School, 2Faculty of Oncology, Medical School, 3State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, China*These authors contributed equally to this workAbstract: Traditional Chinese medicine is gradually becoming a new source of anticancer drugs. One such example is wogonin, which is cytotoxic to various cancer cell lines in vitro. However, due to its low water solubility, wogonin is restricted to clinical administration. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention. In this study, wogonin was conjugated with the drug delivery system of MNPs by mechanical absorption polymerization to fabricate wogonin-loaded MNPs. It was demonstrated that MNPs could strengthen wogonin-induced cell inhibition, apoptosis, and cell cycle arrest in Raji cells by methylthiazol tetrazolium assay, flow cytometer assay, and nuclear 4&#39;,6-diamidino-2-phenylindole staining. Furthermore, the molecular mechanisms of these phenomena were explored by western blot, in which the protein levels of caspase 8 and caspase 3 were increased significantly while those of survivin and cyclin E were decreased significantly in wogonin-MNPs group. These findings suggest that the combination of wogonin and MNPs provides a promising strategy for lymphoma therapy.Keywords: wogonin, magnetic nanoparticles, Raji cell, apoptosis, cell cycle, caspase 8, caspase 3, survivin, cyclin EWang XMLiu RCai XHXu PPRen YYTian LBao WGao CShao ZCheng JWang SXia GHChen BAZhang HJWang LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 789-798 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang XM Liu R Cai XH Xu PP Ren YY Tian L Bao W Gao C Shao Z Cheng J Wang S Xia GH Chen BA Zhang HJ Wang L Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells
description	Lei Wang1,2,, Haijun Zhang1,2,, Baoan Chen1,2, Guohua Xia1,2, Shuai Wang1,2, Jian Cheng1,2, Zeye Shao1,2, Chong Gao1,2, Wen Bao1,2, Liang Tian1,2, Yanyan Ren1,2, Peipei Xu1,2, Xiaohui Cai1,2, Ran Liu1,2, Xuemei Wang3 1Department of Hematology and Oncology, Zhongda Hospital, Medical School, 2Faculty of Oncology, Medical School, 3State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, China*These authors contributed equally to this workAbstract: Traditional Chinese medicine is gradually becoming a new source of anticancer drugs. One such example is wogonin, which is cytotoxic to various cancer cell lines in vitro. However, due to its low water solubility, wogonin is restricted to clinical administration. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention. In this study, wogonin was conjugated with the drug delivery system of MNPs by mechanical absorption polymerization to fabricate wogonin-loaded MNPs. It was demonstrated that MNPs could strengthen wogonin-induced cell inhibition, apoptosis, and cell cycle arrest in Raji cells by methylthiazol tetrazolium assay, flow cytometer assay, and nuclear 4&#39;,6-diamidino-2-phenylindole staining. Furthermore, the molecular mechanisms of these phenomena were explored by western blot, in which the protein levels of caspase 8 and caspase 3 were increased significantly while those of survivin and cyclin E were decreased significantly in wogonin-MNPs group. These findings suggest that the combination of wogonin and MNPs provides a promising strategy for lymphoma therapy.Keywords: wogonin, magnetic nanoparticles, Raji cell, apoptosis, cell cycle, caspase 8, caspase 3, survivin, cyclin E
format	article
author	Wang XM Liu R Cai XH Xu PP Ren YY Tian L Bao W Gao C Shao Z Cheng J Wang S Xia GH Chen BA Zhang HJ Wang L
author_facet	Wang XM Liu R Cai XH Xu PP Ren YY Tian L Bao W Gao C Shao Z Cheng J Wang S Xia GH Chen BA Zhang HJ Wang L
author_sort	Wang XM
title	Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells
title_short	Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells
title_full	Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells
title_fullStr	Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells
title_full_unstemmed	Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells
title_sort	effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in raji cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/1bc7fd136bfa445aa0c593757bb25df9
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Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

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