Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus

This study aimed to compare the test results of anti-double-stranded DNA (anti-dsDNA) antibodies obtained using chemiluminescent immunoassay (CIA) and enzyme-linked immunosorbent assay (ELISA), and investigate predictors of inconsistent results. This retrospective study included 502 patients who und...

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Autores principales:	Huang-Chen Chang, Yen-Ching Wu, Jun-Peng Chen, Yi-Da Wu, Wen-Nan Huang, Yi-Hsing Chen, Yi-Ming Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	systemic lupus erythematosus anti-dsDNA antibody chemiluminescent immunoassay enzyme-linked immunosorbent assay lupus nephritis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1bc944ec3abb4607ad916a4b6e3558ca
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spelling	oai:doaj.org-article:1bc944ec3abb4607ad916a4b6e3558ca2021-11-25T17:20:04ZComparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus10.3390/diagnostics111119402075-4418https://doaj.org/article/1bc944ec3abb4607ad916a4b6e3558ca2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/1940https://doaj.org/toc/2075-4418This study aimed to compare the test results of anti-double-stranded DNA (anti-dsDNA) antibodies obtained using chemiluminescent immunoassay (CIA) and enzyme-linked immunosorbent assay (ELISA), and investigate predictors of inconsistent results. This retrospective study included 502 patients who underwent CIA and ELISA to determine their anti-dsDNA antibody values within a year. We compared the diagnostic power for SLE, disease activity, and predictive power for lupus nephritis (LN). A multivariate analysis was performed to determine the predictors of inconsistencies. CIA and ELISA were moderately correlated in terms of their consistency (Cronbach’s α = 0.571), and yielded comparably favorable results in terms of SLE diagnostic power and SLE disease activity. However, if the patient had LN, CIA displayed higher predictive power than ELISA (0.620 vs. 0.555, <i>p</i> = 0.026). Compared with the CIA/ELISA double-positive group, the inconsistent group had lower anti-C1q circulating immune complexes (CIC) antibody values (OR: 0.42, 95% CI: 0.18–0.94, <i>p</i> = 0.036), and lower SLEDAI scores (≥4) (OR: 0.33, 95% CI: 0.14–0.79, <i>p</i> = 0.013). Anti-dsDNA antibody detection with CIA exhibited higher predictability for diagnosing LN than did ELISA. In the event of inconsistencies between anti-dsDNA methods, SLE disease activity and CIC test values should be considered simultaneously.Huang-Chen ChangYen-Ching WuJun-Peng ChenYi-Da WuWen-Nan HuangYi-Hsing ChenYi-Ming ChenMDPI AGarticlesystemic lupus erythematosusanti-dsDNA antibodychemiluminescent immunoassayenzyme-linked immunosorbent assaylupus nephritisMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 1940, p 1940 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	systemic lupus erythematosus anti-dsDNA antibody chemiluminescent immunoassay enzyme-linked immunosorbent assay lupus nephritis Medicine (General) R5-920
spellingShingle	systemic lupus erythematosus anti-dsDNA antibody chemiluminescent immunoassay enzyme-linked immunosorbent assay lupus nephritis Medicine (General) R5-920 Huang-Chen Chang Yen-Ching Wu Jun-Peng Chen Yi-Da Wu Wen-Nan Huang Yi-Hsing Chen Yi-Ming Chen Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
description	This study aimed to compare the test results of anti-double-stranded DNA (anti-dsDNA) antibodies obtained using chemiluminescent immunoassay (CIA) and enzyme-linked immunosorbent assay (ELISA), and investigate predictors of inconsistent results. This retrospective study included 502 patients who underwent CIA and ELISA to determine their anti-dsDNA antibody values within a year. We compared the diagnostic power for SLE, disease activity, and predictive power for lupus nephritis (LN). A multivariate analysis was performed to determine the predictors of inconsistencies. CIA and ELISA were moderately correlated in terms of their consistency (Cronbach’s α = 0.571), and yielded comparably favorable results in terms of SLE diagnostic power and SLE disease activity. However, if the patient had LN, CIA displayed higher predictive power than ELISA (0.620 vs. 0.555, <i>p</i> = 0.026). Compared with the CIA/ELISA double-positive group, the inconsistent group had lower anti-C1q circulating immune complexes (CIC) antibody values (OR: 0.42, 95% CI: 0.18–0.94, <i>p</i> = 0.036), and lower SLEDAI scores (≥4) (OR: 0.33, 95% CI: 0.14–0.79, <i>p</i> = 0.013). Anti-dsDNA antibody detection with CIA exhibited higher predictability for diagnosing LN than did ELISA. In the event of inconsistencies between anti-dsDNA methods, SLE disease activity and CIC test values should be considered simultaneously.
format	article
author	Huang-Chen Chang Yen-Ching Wu Jun-Peng Chen Yi-Da Wu Wen-Nan Huang Yi-Hsing Chen Yi-Ming Chen
author_facet	Huang-Chen Chang Yen-Ching Wu Jun-Peng Chen Yi-Da Wu Wen-Nan Huang Yi-Hsing Chen Yi-Ming Chen
author_sort	Huang-Chen Chang
title	Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
title_short	Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
title_full	Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
title_fullStr	Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
title_full_unstemmed	Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
title_sort	comparisons of anti-dsdna antibody detection methods by chemiluminescent immunoassay and enzyme-linked immunosorbent assay in systemic lupus erythematosus
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1bc944ec3abb4607ad916a4b6e3558ca
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Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus

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