Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism

Abstract With increased global warming, the impact of high temperature and humidity (HTH) on human health is increasing. Traditional Chinese medicine describes the Herb Yinchen as a remedy for reducing heat and eliminating dampness. This study focused on the impact of HTH conditions on mice and the...

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Autores principales:	Yao Wang, Jiayi Chen, Jianbang Tang, Jiedong Xiao, Yuhua Zheng, Liting Tang, Huanhuan Luo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1bdb0e821f284dbbbd5d6d562eb0f082
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spelling	oai:doaj.org-article:1bdb0e821f284dbbbd5d6d562eb0f0822021-12-02T13:30:51ZCombined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism10.1038/s41598-021-84694-92045-2322https://doaj.org/article/1bdb0e821f284dbbbd5d6d562eb0f0822021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84694-9https://doaj.org/toc/2045-2322Abstract With increased global warming, the impact of high temperature and humidity (HTH) on human health is increasing. Traditional Chinese medicine describes the Herb Yinchen as a remedy for reducing heat and eliminating dampness. This study focused on the impact of HTH conditions on mice and the potential protective effect of Herb Yinchen. Five male Balb/c mouse groups included two normal control groups, two HTH-exposed groups, and one Yinchen-treated group. For either three or ten days, normal and HTH-exposed mice were housed under normal or HTH (33 ± 2 °C,85% relative humidity) conditions, respectively. Yinchen-treated mice, housed under HTH conditions, received the Herb Yinchen decoction for three days. Metabolite profiles of plasma and liver samples from each group were analyzed using LC–MS/MS. Fecal DNA was extracted for 16S rDNA analysis to evaluate the intestinal microbiome. Spearman correlation analysis was performed on metabolites, bacteria, and bile acids that differed between the groups. We found that HTH altered the host metabolite profiles and reduced microbial diversity, causing intestinal microbiome imbalance. Interestingly, Herb Yinchen treatment improved HTH-mediated changes of the metabolite profiles and the intestinal microbiome, restoring them to values observed in normal controls. In conclusion, our study reveals that HTH causes intestinal bacterial disturbances and metabolic disorders in normal mice, while Herb Yinchen could afford protection against such changes.Yao WangJiayi ChenJianbang TangJiedong XiaoYuhua ZhengLiting TangHuanhuan LuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yao Wang Jiayi Chen Jianbang Tang Jiedong Xiao Yuhua Zheng Liting Tang Huanhuan Luo Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism
description	Abstract With increased global warming, the impact of high temperature and humidity (HTH) on human health is increasing. Traditional Chinese medicine describes the Herb Yinchen as a remedy for reducing heat and eliminating dampness. This study focused on the impact of HTH conditions on mice and the potential protective effect of Herb Yinchen. Five male Balb/c mouse groups included two normal control groups, two HTH-exposed groups, and one Yinchen-treated group. For either three or ten days, normal and HTH-exposed mice were housed under normal or HTH (33 ± 2 °C,85% relative humidity) conditions, respectively. Yinchen-treated mice, housed under HTH conditions, received the Herb Yinchen decoction for three days. Metabolite profiles of plasma and liver samples from each group were analyzed using LC–MS/MS. Fecal DNA was extracted for 16S rDNA analysis to evaluate the intestinal microbiome. Spearman correlation analysis was performed on metabolites, bacteria, and bile acids that differed between the groups. We found that HTH altered the host metabolite profiles and reduced microbial diversity, causing intestinal microbiome imbalance. Interestingly, Herb Yinchen treatment improved HTH-mediated changes of the metabolite profiles and the intestinal microbiome, restoring them to values observed in normal controls. In conclusion, our study reveals that HTH causes intestinal bacterial disturbances and metabolic disorders in normal mice, while Herb Yinchen could afford protection against such changes.
format	article
author	Yao Wang Jiayi Chen Jianbang Tang Jiedong Xiao Yuhua Zheng Liting Tang Huanhuan Luo
author_facet	Yao Wang Jiayi Chen Jianbang Tang Jiedong Xiao Yuhua Zheng Liting Tang Huanhuan Luo
author_sort	Yao Wang
title	Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism
title_short	Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism
title_full	Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism
title_fullStr	Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism
title_full_unstemmed	Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism
title_sort	combined lc–ms/ms and 16s rdna analysis on mice under high temperature and humidity and herb yinchen protection mechanism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1bdb0e821f284dbbbd5d6d562eb0f082
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Combined LC–MS/MS and 16S rDNA analysis on mice under high temperature and humidity and Herb Yinchen protection mechanism

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