Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.

Highly pathogenic avian influenza viruses (HPAIV) of subtype H5N1 not only cause a devastating disease in domestic chickens and turkeys but also pose a continuous threat to public health. In some countries, H5N1 viruses continue to circulate and evolve into new clades and subclades. The rapid evolut...

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Autores principales:	Stefan J Halbherr, Terza Brostoff, Merve Tippenhauer, Samira Locher, Marianne Berger Rentsch, Gert Zimmer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1be8e769c1e54d17b7f93fa0fde7b393
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spelling	oai:doaj.org-article:1be8e769c1e54d17b7f93fa0fde7b3932021-11-18T07:42:27ZVaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.1932-620310.1371/journal.pone.0066059https://doaj.org/article/1be8e769c1e54d17b7f93fa0fde7b3932013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23762463/?tool=EBIhttps://doaj.org/toc/1932-6203Highly pathogenic avian influenza viruses (HPAIV) of subtype H5N1 not only cause a devastating disease in domestic chickens and turkeys but also pose a continuous threat to public health. In some countries, H5N1 viruses continue to circulate and evolve into new clades and subclades. The rapid evolution of these viruses represents a problem for virus diagnosis and control. In this work, recombinant vesicular stomatitis virus (VSV) vectors expressing HA of subtype H5 were generated. To comply with biosafety issues the G gene was deleted from the VSV genome. The resulting vaccine vector VSV*ΔG(HA) was propagated on helper cells providing the VSV G protein in trans. Vaccination of chickens with a single intramuscular dose of 2×10⁸ infectious replicon particles without adjuvant conferred complete protection from lethal H5N1 infection. Subsequent application of the same vaccine strongly boosted the humoral immune response and completely prevented shedding of challenge virus and transmission to sentinel birds. The vaccine allowed serological differentiation of infected from vaccinated animals (DIVA) by employing a commercially available ELISA. Immunized chickens produced antibodies with neutralizing activity against multiple H5 viruses representing clades 1, 2.2, 2.5, and low-pathogenic avian influenza viruses (classical clade). Studies using chimeric H1/H5 hemagglutinins showed that the neutralizing activity was predominantly directed against the globular head domain. In summary, these results suggest that VSV replicon particles are safe and potent DIVA vaccines that may help to control avian influenza viruses in domestic poultry.Stefan J HalbherrTerza BrostoffMerve TippenhauerSamira LocherMarianne Berger RentschGert ZimmerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66059 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan J Halbherr Terza Brostoff Merve Tippenhauer Samira Locher Marianne Berger Rentsch Gert Zimmer Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.
description	Highly pathogenic avian influenza viruses (HPAIV) of subtype H5N1 not only cause a devastating disease in domestic chickens and turkeys but also pose a continuous threat to public health. In some countries, H5N1 viruses continue to circulate and evolve into new clades and subclades. The rapid evolution of these viruses represents a problem for virus diagnosis and control. In this work, recombinant vesicular stomatitis virus (VSV) vectors expressing HA of subtype H5 were generated. To comply with biosafety issues the G gene was deleted from the VSV genome. The resulting vaccine vector VSV*ΔG(HA) was propagated on helper cells providing the VSV G protein in trans. Vaccination of chickens with a single intramuscular dose of 2×10⁸ infectious replicon particles without adjuvant conferred complete protection from lethal H5N1 infection. Subsequent application of the same vaccine strongly boosted the humoral immune response and completely prevented shedding of challenge virus and transmission to sentinel birds. The vaccine allowed serological differentiation of infected from vaccinated animals (DIVA) by employing a commercially available ELISA. Immunized chickens produced antibodies with neutralizing activity against multiple H5 viruses representing clades 1, 2.2, 2.5, and low-pathogenic avian influenza viruses (classical clade). Studies using chimeric H1/H5 hemagglutinins showed that the neutralizing activity was predominantly directed against the globular head domain. In summary, these results suggest that VSV replicon particles are safe and potent DIVA vaccines that may help to control avian influenza viruses in domestic poultry.
format	article
author	Stefan J Halbherr Terza Brostoff Merve Tippenhauer Samira Locher Marianne Berger Rentsch Gert Zimmer
author_facet	Stefan J Halbherr Terza Brostoff Merve Tippenhauer Samira Locher Marianne Berger Rentsch Gert Zimmer
author_sort	Stefan J Halbherr
title	Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.
title_short	Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.
title_full	Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.
title_fullStr	Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.
title_full_unstemmed	Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.
title_sort	vaccination with recombinant rna replicon particles protects chickens from h5n1 highly pathogenic avian influenza virus.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1be8e769c1e54d17b7f93fa0fde7b393
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Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.

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