Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells
This paper presents a partially coherent point-diffraction digital holographic microscopy (PC-pDHM) prototype and demonstrates its application in label-free imaging of the dynamic processes of live cells. In PC-pDHM, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/1bef43df89cb4b609727221aeff8c2c4 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:1bef43df89cb4b609727221aeff8c2c4 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:1bef43df89cb4b609727221aeff8c2c42021-11-22T07:22:14ZPartially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells2296-424X10.3389/fphy.2021.796935https://doaj.org/article/1bef43df89cb4b609727221aeff8c2c42021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphy.2021.796935/fullhttps://doaj.org/toc/2296-424XThis paper presents a partially coherent point-diffraction digital holographic microscopy (PC-pDHM) prototype and demonstrates its application in label-free imaging of the dynamic processes of live cells. In PC-pDHM, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the partially coherent illumination (PCI), which reduces the speckle noise significantly in PC-pDHM. A polarization-grating is used to remold the object and the reference waves, and the fringe contrast of the generated hologram can be adjusted by changing the polarization of the illumination wave. Using the PC-pDHM prototype, transparent samples and notably the dynamic processes of live cells were imaged with high contrast and in a label-free manner, discovering the pathological mechanisms of biology in the cellular and sub-cellular levels.Kequn ZhuoYu WangYang WangKai WenMin LiuMin LiuYing MaYing MaJuanjuan ZhengJuanjuan ZhengJuanjuan ZhengPeng GaoFrontiers Media S.A.articlequantitative phase microscopy (QPM)digital holographic microscopy (DHM)point-diffractionpartially coherent illuminationlive-cell imagingPhysicsQC1-999ENFrontiers in Physics, Vol 9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
quantitative phase microscopy (QPM) digital holographic microscopy (DHM) point-diffraction partially coherent illumination live-cell imaging Physics QC1-999 |
| spellingShingle |
quantitative phase microscopy (QPM) digital holographic microscopy (DHM) point-diffraction partially coherent illumination live-cell imaging Physics QC1-999 Kequn Zhuo Yu Wang Yang Wang Kai Wen Min Liu Min Liu Ying Ma Ying Ma Juanjuan Zheng Juanjuan Zheng Juanjuan Zheng Peng Gao Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells |
| description |
This paper presents a partially coherent point-diffraction digital holographic microscopy (PC-pDHM) prototype and demonstrates its application in label-free imaging of the dynamic processes of live cells. In PC-pDHM, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the partially coherent illumination (PCI), which reduces the speckle noise significantly in PC-pDHM. A polarization-grating is used to remold the object and the reference waves, and the fringe contrast of the generated hologram can be adjusted by changing the polarization of the illumination wave. Using the PC-pDHM prototype, transparent samples and notably the dynamic processes of live cells were imaged with high contrast and in a label-free manner, discovering the pathological mechanisms of biology in the cellular and sub-cellular levels. |
| format |
article |
| author |
Kequn Zhuo Yu Wang Yang Wang Kai Wen Min Liu Min Liu Ying Ma Ying Ma Juanjuan Zheng Juanjuan Zheng Juanjuan Zheng Peng Gao |
| author_facet |
Kequn Zhuo Yu Wang Yang Wang Kai Wen Min Liu Min Liu Ying Ma Ying Ma Juanjuan Zheng Juanjuan Zheng Juanjuan Zheng Peng Gao |
| author_sort |
Kequn Zhuo |
| title |
Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells |
| title_short |
Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells |
| title_full |
Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells |
| title_fullStr |
Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells |
| title_full_unstemmed |
Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells |
| title_sort |
partially coherent illumination based point-diffraction digital holographic microscopy study dynamics of live cells |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/1bef43df89cb4b609727221aeff8c2c4 |
| work_keys_str_mv |
AT kequnzhuo partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT yuwang partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT yangwang partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT kaiwen partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT minliu partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT minliu partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT yingma partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT yingma partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT juanjuanzheng partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT juanjuanzheng partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT juanjuanzheng partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells AT penggao partiallycoherentilluminationbasedpointdiffractiondigitalholographicmicroscopystudydynamicsoflivecells |
| _version_ |
1718417842989170688 |