Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration

Abstract Electrical stimulation has been suggested as a means for promoting the direct structural and functional bonding of bone tissue to an artificial implant, known as osseointegration. Previous work has investigated the impact of electrical stimulation in different models, both in vitro and in v...

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Autores principales:	Emily Pettersen, Furqan A. Shah, Max Ortiz-Catalan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1befa23304224e168d48e6fdf977e426
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spelling	oai:doaj.org-article:1befa23304224e168d48e6fdf977e4262021-11-21T12:19:14ZEnhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration10.1038/s41598-021-01901-32045-2322https://doaj.org/article/1befa23304224e168d48e6fdf977e4262021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01901-3https://doaj.org/toc/2045-2322Abstract Electrical stimulation has been suggested as a means for promoting the direct structural and functional bonding of bone tissue to an artificial implant, known as osseointegration. Previous work has investigated the impact of electrical stimulation in different models, both in vitro and in vivo, using various electrode configurations for inducing an electric field with a wide range of stimulation parameters. However, there is no consensus on optimal electrode configuration nor stimulation parameters. Here, we investigated a novel approach of delivering electrical stimulation to a titanium implant using parameters clinically tested in a different application, namely peripheral nerve stimulation. We propose an in vitro model comprising of Ti6Al4V implants precultured with MC3T3-E1 preosteoblasts, stimulated for 72 h at two different pulse amplitudes (10 µA and 20 µA) and at two different frequencies (50 Hz and 100 Hz). We found that asymmetric charge-balanced pulsed electrical stimulation improved cell survival and collagen production in a dose-dependent manner. Our findings suggest that pulsed electrical stimulation with characteristics similar to peripheral nerve stimulation has the potential to improve cell survival and may provide a promising approach to improve peri-implant bone healing, particularly to neuromusculoskeletal interfaces in which implanted electrodes are readily available.Emily PettersenFurqan A. ShahMax Ortiz-CatalanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emily Pettersen Furqan A. Shah Max Ortiz-Catalan Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration
description	Abstract Electrical stimulation has been suggested as a means for promoting the direct structural and functional bonding of bone tissue to an artificial implant, known as osseointegration. Previous work has investigated the impact of electrical stimulation in different models, both in vitro and in vivo, using various electrode configurations for inducing an electric field with a wide range of stimulation parameters. However, there is no consensus on optimal electrode configuration nor stimulation parameters. Here, we investigated a novel approach of delivering electrical stimulation to a titanium implant using parameters clinically tested in a different application, namely peripheral nerve stimulation. We propose an in vitro model comprising of Ti6Al4V implants precultured with MC3T3-E1 preosteoblasts, stimulated for 72 h at two different pulse amplitudes (10 µA and 20 µA) and at two different frequencies (50 Hz and 100 Hz). We found that asymmetric charge-balanced pulsed electrical stimulation improved cell survival and collagen production in a dose-dependent manner. Our findings suggest that pulsed electrical stimulation with characteristics similar to peripheral nerve stimulation has the potential to improve cell survival and may provide a promising approach to improve peri-implant bone healing, particularly to neuromusculoskeletal interfaces in which implanted electrodes are readily available.
format	article
author	Emily Pettersen Furqan A. Shah Max Ortiz-Catalan
author_facet	Emily Pettersen Furqan A. Shah Max Ortiz-Catalan
author_sort	Emily Pettersen
title	Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration
title_short	Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration
title_full	Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration
title_fullStr	Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration
title_full_unstemmed	Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration
title_sort	enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1befa23304224e168d48e6fdf977e426
work_keys_str_mv	AT emilypettersen enhancingosteoblastsurvivalthroughpulsedelectricalstimulationandimplicationsforosseointegration AT furqanashah enhancingosteoblastsurvivalthroughpulsedelectricalstimulationandimplicationsforosseointegration AT maxortizcatalan enhancingosteoblastsurvivalthroughpulsedelectricalstimulationandimplicationsforosseointegration
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Enhancing osteoblast survival through pulsed electrical stimulation and implications for osseointegration

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