Quantitation of the cellular content of saliva and buccal swab samples

Abstract Buccal swabs and saliva are the two most common oral sampling methods used for medical research. Often, these samples are used interchangeably, despite previous evidence that both contain buccal cells and blood leukocytes in different proportions. For some research, such as epigenetic studi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christiane Theda, Seo Hye Hwang, Anna Czajko, Yuk Jing Loke, Pamela Leong, Jeffrey M. Craig
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1bf2092b0a9446bd8819ebbc277bd7e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1bf2092b0a9446bd8819ebbc277bd7e9
record_format	dspace
spelling	oai:doaj.org-article:1bf2092b0a9446bd8819ebbc277bd7e92021-12-02T15:07:59ZQuantitation of the cellular content of saliva and buccal swab samples10.1038/s41598-018-25311-02045-2322https://doaj.org/article/1bf2092b0a9446bd8819ebbc277bd7e92018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25311-0https://doaj.org/toc/2045-2322Abstract Buccal swabs and saliva are the two most common oral sampling methods used for medical research. Often, these samples are used interchangeably, despite previous evidence that both contain buccal cells and blood leukocytes in different proportions. For some research, such as epigenetic studies, the cell types contributing to the analysis are highly relevant. We collected such samples from twelve children and twenty adults and, using Papanicolaou staining, measured the proportions of epithelial cells and leukocytes through microscopy. To our knowledge, no studies have compared cellular heterogeneity in buccal swab and saliva samples from adults and children. We confirmed that buccal swabs contained a higher proportion of epithelial cells than saliva and that children have a greater proportion of such cells in saliva compared to adults. At this level of resolution, buccal swabs and saliva contained similar epithelial cell subtypes. Gingivitis in children was associated with a higher proportion of leukocytes in saliva samples but not in buccal swabs. Compared to more detailed and costly methods such as flow cytometry or deconvolution methods used in epigenomic analysis, the procedure described here can serve as a simple and low-cost method to characterize buccal and saliva samples. Microscopy provides a low-cost tool to alert researchers to the presence of oral inflammation which may affect a subset of their samples. This knowledge might be highly relevant to their specific research questions, may assist with sample selection and thus might be crucial information despite the ability of data deconvolution methods to correct for cellular heterogeneity.Christiane ThedaSeo Hye HwangAnna CzajkoYuk Jing LokePamela LeongJeffrey M. CraigNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christiane Theda Seo Hye Hwang Anna Czajko Yuk Jing Loke Pamela Leong Jeffrey M. Craig Quantitation of the cellular content of saliva and buccal swab samples
description	Abstract Buccal swabs and saliva are the two most common oral sampling methods used for medical research. Often, these samples are used interchangeably, despite previous evidence that both contain buccal cells and blood leukocytes in different proportions. For some research, such as epigenetic studies, the cell types contributing to the analysis are highly relevant. We collected such samples from twelve children and twenty adults and, using Papanicolaou staining, measured the proportions of epithelial cells and leukocytes through microscopy. To our knowledge, no studies have compared cellular heterogeneity in buccal swab and saliva samples from adults and children. We confirmed that buccal swabs contained a higher proportion of epithelial cells than saliva and that children have a greater proportion of such cells in saliva compared to adults. At this level of resolution, buccal swabs and saliva contained similar epithelial cell subtypes. Gingivitis in children was associated with a higher proportion of leukocytes in saliva samples but not in buccal swabs. Compared to more detailed and costly methods such as flow cytometry or deconvolution methods used in epigenomic analysis, the procedure described here can serve as a simple and low-cost method to characterize buccal and saliva samples. Microscopy provides a low-cost tool to alert researchers to the presence of oral inflammation which may affect a subset of their samples. This knowledge might be highly relevant to their specific research questions, may assist with sample selection and thus might be crucial information despite the ability of data deconvolution methods to correct for cellular heterogeneity.
format	article
author	Christiane Theda Seo Hye Hwang Anna Czajko Yuk Jing Loke Pamela Leong Jeffrey M. Craig
author_facet	Christiane Theda Seo Hye Hwang Anna Czajko Yuk Jing Loke Pamela Leong Jeffrey M. Craig
author_sort	Christiane Theda
title	Quantitation of the cellular content of saliva and buccal swab samples
title_short	Quantitation of the cellular content of saliva and buccal swab samples
title_full	Quantitation of the cellular content of saliva and buccal swab samples
title_fullStr	Quantitation of the cellular content of saliva and buccal swab samples
title_full_unstemmed	Quantitation of the cellular content of saliva and buccal swab samples
title_sort	quantitation of the cellular content of saliva and buccal swab samples
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1bf2092b0a9446bd8819ebbc277bd7e9
work_keys_str_mv	AT christianetheda quantitationofthecellularcontentofsalivaandbuccalswabsamples AT seohyehwang quantitationofthecellularcontentofsalivaandbuccalswabsamples AT annaczajko quantitationofthecellularcontentofsalivaandbuccalswabsamples AT yukjingloke quantitationofthecellularcontentofsalivaandbuccalswabsamples AT pamelaleong quantitationofthecellularcontentofsalivaandbuccalswabsamples AT jeffreymcraig quantitationofthecellularcontentofsalivaandbuccalswabsamples
_version_	1718388302395998208

Quantitation of the cellular content of saliva and buccal swab samples

Ejemplares similares