Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction

In this paper, the authors report the directed-evolution of translation systems that allow the incorporation of unnatural amino acids with similar efficiency to natural amino acids and the construction of synthetic auxotroph in a generalizable way.

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Detalles Bibliográficos
Autores principales: Hongxia Zhao, Wenlong Ding, Jia Zang, Yang Yang, Chao Liu, Linzhen Hu, Yulin Chen, Guanglong Liu, Yu Fang, Ying Yuan, Shixian Lin
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/1bf4b6f580834b37bd653778889991d8
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Sumario:In this paper, the authors report the directed-evolution of translation systems that allow the incorporation of unnatural amino acids with similar efficiency to natural amino acids and the construction of synthetic auxotroph in a generalizable way.