Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides

Four bacterial strains were isolated from enrichment cultures inoculated with soil from Bien Hoa military base in Vietnam contaminated with the herbicides 2,4-dichlorophenoxyacetate (2,4-D) and 2,4,5-trichlorophenoxyacetate (2,4,5-T). They were classified as Pseudomonas aeruginosa BT1 2.2, Sphingomo...

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Autores principales:	Thi Lan Anh Nguyen, Ha Thi Cam Dang, Jacco Koekkoek, Martin Braster, John R. Parsons, Abraham Brouwer, Tjalf de Boer, Rob J. M. van Spanning
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioremediation Agent Orange tfd gene clusters horizontal gene transfer (HGT) chlorophenoxy acid herbicides Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/1c0136c98049470491939820bf0cd616
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spelling	oai:doaj.org-article:1c0136c98049470491939820bf0cd6162021-12-01T08:22:53ZSpecies and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides2624-963410.3389/frsc.2021.692018https://doaj.org/article/1c0136c98049470491939820bf0cd6162021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/frsc.2021.692018/fullhttps://doaj.org/toc/2624-9634Four bacterial strains were isolated from enrichment cultures inoculated with soil from Bien Hoa military base in Vietnam contaminated with the herbicides 2,4-dichlorophenoxyacetate (2,4-D) and 2,4,5-trichlorophenoxyacetate (2,4,5-T). They were classified as Pseudomonas aeruginosa BT1 2.2, Sphingomonas histidinilytica BT1 5.2, Bordetella petrii BT1 9.2, and Achromobacter xylosoxidans BT1 10.2. All four were able to degrade 2,4-D and 2,4,5-T, but only the last three species used them as the sole sources of carbon and energy. Mass balance analyses suggest that between 33 and 46% of the carbon in the herbicides is incorporated into dry weight (DW). We obtained insight into their degradation pathways by the genomic analysis of these strains. A tfdCDEF gene cluster was found in A. xylosoxidans BT1 10.2 with amino acid sequences of their gene products showing high identity to those in B. petrii DSM12804. Bordetella petrii BT1 9.2 has a full complement of the tfdABCDEF genes. Surprisingly, the gene organization along with the amino acid sequences of the gene products are virtually identical to those of Cupriavidus pinatubonensis JMP134, referred to as type I tfd genes, and different from those of A. xylosoxidans BT1 10.2 and B. petrii DSM12804. We hypothesize that some of the genetic potential to degrade the herbicides has been recruited in recent mating events between these species and other members of the proteobacteria. This is the first report showing that B. petrii BT1 9.2 emerges as a key player in the degradation of 2,4-D.Thi Lan Anh NguyenThi Lan Anh NguyenHa Thi Cam DangJacco KoekkoekMartin BrasterJohn R. ParsonsAbraham BrouwerAbraham BrouwerTjalf de BoerRob J. M. van SpanningFrontiers Media S.A.articlebioremediationAgent Orangetfd gene clustershorizontal gene transfer (HGT)chlorophenoxy acid herbicidesScience (General)Q1-390Social sciences (General)H1-99ENFrontiers in Sustainable Cities, Vol 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioremediation Agent Orange tfd gene clusters horizontal gene transfer (HGT) chlorophenoxy acid herbicides Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	bioremediation Agent Orange tfd gene clusters horizontal gene transfer (HGT) chlorophenoxy acid herbicides Science (General) Q1-390 Social sciences (General) H1-99 Thi Lan Anh Nguyen Thi Lan Anh Nguyen Ha Thi Cam Dang Jacco Koekkoek Martin Braster John R. Parsons Abraham Brouwer Abraham Brouwer Tjalf de Boer Rob J. M. van Spanning Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides
description	Four bacterial strains were isolated from enrichment cultures inoculated with soil from Bien Hoa military base in Vietnam contaminated with the herbicides 2,4-dichlorophenoxyacetate (2,4-D) and 2,4,5-trichlorophenoxyacetate (2,4,5-T). They were classified as Pseudomonas aeruginosa BT1 2.2, Sphingomonas histidinilytica BT1 5.2, Bordetella petrii BT1 9.2, and Achromobacter xylosoxidans BT1 10.2. All four were able to degrade 2,4-D and 2,4,5-T, but only the last three species used them as the sole sources of carbon and energy. Mass balance analyses suggest that between 33 and 46% of the carbon in the herbicides is incorporated into dry weight (DW). We obtained insight into their degradation pathways by the genomic analysis of these strains. A tfdCDEF gene cluster was found in A. xylosoxidans BT1 10.2 with amino acid sequences of their gene products showing high identity to those in B. petrii DSM12804. Bordetella petrii BT1 9.2 has a full complement of the tfdABCDEF genes. Surprisingly, the gene organization along with the amino acid sequences of the gene products are virtually identical to those of Cupriavidus pinatubonensis JMP134, referred to as type I tfd genes, and different from those of A. xylosoxidans BT1 10.2 and B. petrii DSM12804. We hypothesize that some of the genetic potential to degrade the herbicides has been recruited in recent mating events between these species and other members of the proteobacteria. This is the first report showing that B. petrii BT1 9.2 emerges as a key player in the degradation of 2,4-D.
format	article
author	Thi Lan Anh Nguyen Thi Lan Anh Nguyen Ha Thi Cam Dang Jacco Koekkoek Martin Braster John R. Parsons Abraham Brouwer Abraham Brouwer Tjalf de Boer Rob J. M. van Spanning
author_facet	Thi Lan Anh Nguyen Thi Lan Anh Nguyen Ha Thi Cam Dang Jacco Koekkoek Martin Braster John R. Parsons Abraham Brouwer Abraham Brouwer Tjalf de Boer Rob J. M. van Spanning
author_sort	Thi Lan Anh Nguyen
title	Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides
title_short	Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides
title_full	Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides
title_fullStr	Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides
title_full_unstemmed	Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides
title_sort	species and metabolic pathways involved in bioremediation of vietnamese soil from bien hoa airbase contaminated with herbicides
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1c0136c98049470491939820bf0cd616
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Species and Metabolic Pathways Involved in Bioremediation of Vietnamese Soil From Bien Hoa Airbase Contaminated With Herbicides

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