Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model

Abstract Background Allergic asthma is a chronic airway inflammatory disease with a number of cytokines participating in its pathogenesis and progress. Interleukin (IL)-22, which is derived from lymphocytes, acts on epithelial cells and play a role in the chronic airway inflammation. However, the ac...

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Autores principales:	Jingru Wang, Shengnan Gao, Jingyuan Zhang, Chunxiao Li, Hongwen Li, Jiangtao Lin
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Allergic asthma Cytokines Interleukin-22 Animal model Ovalbumin sensitization Diseases of the respiratory system RC705-779
Acceso en línea:	https://doaj.org/article/1c0695ce253e49dc835abd7d52faa60a
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spelling	oai:doaj.org-article:1c0695ce253e49dc835abd7d52faa60a2021-11-28T12:36:33ZInterleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model10.1186/s12890-021-01698-x1471-2466https://doaj.org/article/1c0695ce253e49dc835abd7d52faa60a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12890-021-01698-xhttps://doaj.org/toc/1471-2466Abstract Background Allergic asthma is a chronic airway inflammatory disease with a number of cytokines participating in its pathogenesis and progress. Interleukin (IL)-22, which is derived from lymphocytes, acts on epithelial cells and play a role in the chronic airway inflammation. However, the actual role of IL-22 in allergic asthma is still unclear. Therefore, we explored the effect of IL-22 on allergic airway inflammation and airway hyperresponsiveness (AHR) in an ovalbumin (OVA)-induced asthma mouse model. Methods To evaluate the effect of IL-22 in an allergic asthma model, BALB/c mice were sensitized and challenged with OVA; then the recombinant mouse IL-22 was administered intranasally 24 h prior to each challenge. The IL-22 levels in lung homogenates and bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay, respectively. AHR was evaluated through indicators including airways resistance (Rrs), elastance (Ers) and compliance (Crs); the inflammatory cell infiltration was assessed by quantification of differential cells counts in BALF and lung tissues stained by hematoxylin and eosin (H&E); IL-22 specific receptors were determined by immunohistochemistry staining. Results The concentration of IL-22 was significantly elevated in the OVA-induced mice compared with the control mice in lung homogenates and BALF. In the OVA-induced mouse model, IL-22 administration could significantly attenuate AHR, including Rrs, Ers and Crs, decrease the proportion of eosinophils in BALF and reduce inflammatory cell infiltration around bronchi and their concomitant vessels, compared with the OVA-induced group. In addition, the expression of IL-22RA1 and IL-10RB in the lung tissues of OVA-induced mice was significantly increased compared with the control mice, while it was dramatically decreased after the treatment with IL-22, but not completely attenuated in the IL-22-treated mice when compared with the control mice. Conclusion Interleukin-22 could play a protective role in an OVA-induced asthma model, by suppressing the inflammatory cell infiltration around bronchi and their concomitant vessels and airway hyperresponsiveness, which might associate with the expression of its heterodimer receptors. Thus, IL-22 administration might be an effective strategy to attenuate allergic airway inflammation.Jingru WangShengnan GaoJingyuan ZhangChunxiao LiHongwen LiJiangtao LinBMCarticleAllergic asthmaCytokinesInterleukin-22Animal modelOvalbumin sensitizationDiseases of the respiratory systemRC705-779ENBMC Pulmonary Medicine, Vol 21, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Allergic asthma Cytokines Interleukin-22 Animal model Ovalbumin sensitization Diseases of the respiratory system RC705-779
spellingShingle	Allergic asthma Cytokines Interleukin-22 Animal model Ovalbumin sensitization Diseases of the respiratory system RC705-779 Jingru Wang Shengnan Gao Jingyuan Zhang Chunxiao Li Hongwen Li Jiangtao Lin Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model
description	Abstract Background Allergic asthma is a chronic airway inflammatory disease with a number of cytokines participating in its pathogenesis and progress. Interleukin (IL)-22, which is derived from lymphocytes, acts on epithelial cells and play a role in the chronic airway inflammation. However, the actual role of IL-22 in allergic asthma is still unclear. Therefore, we explored the effect of IL-22 on allergic airway inflammation and airway hyperresponsiveness (AHR) in an ovalbumin (OVA)-induced asthma mouse model. Methods To evaluate the effect of IL-22 in an allergic asthma model, BALB/c mice were sensitized and challenged with OVA; then the recombinant mouse IL-22 was administered intranasally 24 h prior to each challenge. The IL-22 levels in lung homogenates and bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay, respectively. AHR was evaluated through indicators including airways resistance (Rrs), elastance (Ers) and compliance (Crs); the inflammatory cell infiltration was assessed by quantification of differential cells counts in BALF and lung tissues stained by hematoxylin and eosin (H&E); IL-22 specific receptors were determined by immunohistochemistry staining. Results The concentration of IL-22 was significantly elevated in the OVA-induced mice compared with the control mice in lung homogenates and BALF. In the OVA-induced mouse model, IL-22 administration could significantly attenuate AHR, including Rrs, Ers and Crs, decrease the proportion of eosinophils in BALF and reduce inflammatory cell infiltration around bronchi and their concomitant vessels, compared with the OVA-induced group. In addition, the expression of IL-22RA1 and IL-10RB in the lung tissues of OVA-induced mice was significantly increased compared with the control mice, while it was dramatically decreased after the treatment with IL-22, but not completely attenuated in the IL-22-treated mice when compared with the control mice. Conclusion Interleukin-22 could play a protective role in an OVA-induced asthma model, by suppressing the inflammatory cell infiltration around bronchi and their concomitant vessels and airway hyperresponsiveness, which might associate with the expression of its heterodimer receptors. Thus, IL-22 administration might be an effective strategy to attenuate allergic airway inflammation.
format	article
author	Jingru Wang Shengnan Gao Jingyuan Zhang Chunxiao Li Hongwen Li Jiangtao Lin
author_facet	Jingru Wang Shengnan Gao Jingyuan Zhang Chunxiao Li Hongwen Li Jiangtao Lin
author_sort	Jingru Wang
title	Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model
title_short	Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model
title_full	Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model
title_fullStr	Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model
title_full_unstemmed	Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model
title_sort	interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1c0695ce253e49dc835abd7d52faa60a
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_version_	1718407877787385856

Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model

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