Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.

<h4>Background</h4>The genus Aquilegia is an emerging model system in plant evolutionary biology predominantly because of its wide variation in floral traits and associated floral ecology. The anatomy of the Aquilegia flower is also very distinct. There are two whorls of petaloid organs,...

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Autores principales:	Claudia Voelckel, Justin O Borevitz, Elena M Kramer, Scott A Hodges
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c0e98b59524444ea0bc87c56f0e2cc4
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spelling	oai:doaj.org-article:1c0e98b59524444ea0bc87c56f0e2cc42021-11-25T06:25:13ZWithin and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.1932-620310.1371/journal.pone.0009735https://doaj.org/article/1c0e98b59524444ea0bc87c56f0e2cc42010-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20352114/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The genus Aquilegia is an emerging model system in plant evolutionary biology predominantly because of its wide variation in floral traits and associated floral ecology. The anatomy of the Aquilegia flower is also very distinct. There are two whorls of petaloid organs, the outer whorl of sepals and the second whorl of petals that form nectar spurs, as well as a recently evolved fifth whorl of staminodia inserted between stamens and carpels.<h4>Methodology/principal findings</h4>We designed an oligonucleotide microarray based on EST sequences from a mixed tissue, normalized cDNA library of an A. formosa x A. pubescens F2 population representing 17,246 unigenes. We then used this array to analyze floral gene expression in late pre-anthesis stage floral organs from a natural A. formosa population. In particular, we tested for gene expression patterns specific to each floral whorl and to combinations of whorls that correspond to traditional and modified ABC model groupings. Similar analyses were performed on gene expression data of Arabidopsis thaliana whorls previously obtained using the Ath1 gene chips (data available through The Arabidopsis Information Resource).<h4>Conclusions/significance</h4>Our comparative gene expression analyses suggest that 1) petaloid sepals and petals of A. formosa share gene expression patterns more than either have organ-specific patterns, 2) petals of A. formosa and A. thaliana may be independently derived, 3) staminodia express B and C genes similar to stamens but the staminodium genetic program has also converged on aspects of the carpel program and 4) staminodia have unique up-regulation of regulatory genes and genes that have been implicated with defense against microbial infection and herbivory. Our study also highlights the value of comparative gene expression profiling and the Aquilegia microarray in particular for the study of floral evolution and ecology.Claudia VoelckelJustin O BorevitzElena M KramerScott A HodgesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 3, p e9735 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claudia Voelckel Justin O Borevitz Elena M Kramer Scott A Hodges Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.
description	<h4>Background</h4>The genus Aquilegia is an emerging model system in plant evolutionary biology predominantly because of its wide variation in floral traits and associated floral ecology. The anatomy of the Aquilegia flower is also very distinct. There are two whorls of petaloid organs, the outer whorl of sepals and the second whorl of petals that form nectar spurs, as well as a recently evolved fifth whorl of staminodia inserted between stamens and carpels.<h4>Methodology/principal findings</h4>We designed an oligonucleotide microarray based on EST sequences from a mixed tissue, normalized cDNA library of an A. formosa x A. pubescens F2 population representing 17,246 unigenes. We then used this array to analyze floral gene expression in late pre-anthesis stage floral organs from a natural A. formosa population. In particular, we tested for gene expression patterns specific to each floral whorl and to combinations of whorls that correspond to traditional and modified ABC model groupings. Similar analyses were performed on gene expression data of Arabidopsis thaliana whorls previously obtained using the Ath1 gene chips (data available through The Arabidopsis Information Resource).<h4>Conclusions/significance</h4>Our comparative gene expression analyses suggest that 1) petaloid sepals and petals of A. formosa share gene expression patterns more than either have organ-specific patterns, 2) petals of A. formosa and A. thaliana may be independently derived, 3) staminodia express B and C genes similar to stamens but the staminodium genetic program has also converged on aspects of the carpel program and 4) staminodia have unique up-regulation of regulatory genes and genes that have been implicated with defense against microbial infection and herbivory. Our study also highlights the value of comparative gene expression profiling and the Aquilegia microarray in particular for the study of floral evolution and ecology.
format	article
author	Claudia Voelckel Justin O Borevitz Elena M Kramer Scott A Hodges
author_facet	Claudia Voelckel Justin O Borevitz Elena M Kramer Scott A Hodges
author_sort	Claudia Voelckel
title	Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.
title_short	Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.
title_full	Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.
title_fullStr	Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.
title_full_unstemmed	Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.
title_sort	within and between whorls: comparative transcriptional profiling of aquilegia and arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/1c0e98b59524444ea0bc87c56f0e2cc4
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Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis.

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