THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS

Objective: To determine the frequency of Type-able and untype-able genotypes in hepatitis C infected patients, and to observe their association with gender, age, Alanine Aminotranferease and viral load. Study Design: Cross-sectional analytical study. Place and Duration of Study: Department of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qanita Fahim, Irfan Ali Mirza, Ayesha Khalid, Anum Imtiaz, Asad Ahmad
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2021
Materias:	hepatitis c-virus genotypes untype-able variants Medicine R Medicine (General) R5-920
Acceso en línea:	https://doi.org/10.51253/pafmj.v71iSuppl-1.2603 https://doaj.org/article/1c0eb1bfb9dd426c98769c26ec8758c2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1c0eb1bfb9dd426c98769c26ec8758c2
record_format	dspace
spelling	oai:doaj.org-article:1c0eb1bfb9dd426c98769c26ec8758c22021-12-02T15:03:54ZTHE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTShttps://doi.org/10.51253/pafmj.v71iSuppl-1.26030030-96482411-8842https://doaj.org/article/1c0eb1bfb9dd426c98769c26ec8758c22021-01-01T00:00:00Zhttps://pafmj.org/index.php/PAFMJ/article/view/2603https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To determine the frequency of Type-able and untype-able genotypes in hepatitis C infected patients, and to observe their association with gender, age, Alanine Aminotranferease and viral load. Study Design: Cross-sectional analytical study. Place and Duration of Study: Department of Microbiology, Combined Military Hospital, and Lahore, Pakistan from Sep 2017 to Mar 2018. Methodology: Six hundred forty seven anti HCV antibodies positive serum samples by Enzyme Linked Immuno Sorbant Assay were received from a total of 6791 serum samples. The positive sera were subjected to qualitative PCR and quantitative real time (RT) PCR to determine pre-treatment viral load. Quantitative PCR positive sera with viral load >500IU/ml were further subjected to molecular genotyping by using Ohno et al method. Result: Out of 647 positive serum samples, type-specific PCR fragments were seen in 424 sera, while 13 (3.1%) of serum samples were of untype-able genotype. In all age groups genotype 3a had emerged as a predominant genotype 397 (93.6%), followed by 1b 8 (1.9%), 3b 4 (0.9%), 1a 2 (0.5%), while no sample detected to have 2a, 2b, 5a, 6a and mixed genotypes. The highest prevalence of untype-ables were seen in 61-70 age group. Conclusion: Need of the hour is proper sequencing of untype-able genotypes via upgrading existing methodologies. It will not only help the clinicians in achieving sustained virological response but also help in identifying new genotypes/subtypes.Qanita FahimIrfan Ali MirzaAyesha KhalidAnum ImtiazAsad AhmadArmy Medical College Rawalpindiarticlehepatitis c-virusgenotypesuntype-able variantsMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 71, Iss SUPPL-1, Pp 106-112 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis c-virus genotypes untype-able variants Medicine R Medicine (General) R5-920
spellingShingle	hepatitis c-virus genotypes untype-able variants Medicine R Medicine (General) R5-920 Qanita Fahim Irfan Ali Mirza Ayesha Khalid Anum Imtiaz Asad Ahmad THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS
description	Objective: To determine the frequency of Type-able and untype-able genotypes in hepatitis C infected patients, and to observe their association with gender, age, Alanine Aminotranferease and viral load. Study Design: Cross-sectional analytical study. Place and Duration of Study: Department of Microbiology, Combined Military Hospital, and Lahore, Pakistan from Sep 2017 to Mar 2018. Methodology: Six hundred forty seven anti HCV antibodies positive serum samples by Enzyme Linked Immuno Sorbant Assay were received from a total of 6791 serum samples. The positive sera were subjected to qualitative PCR and quantitative real time (RT) PCR to determine pre-treatment viral load. Quantitative PCR positive sera with viral load >500IU/ml were further subjected to molecular genotyping by using Ohno et al method. Result: Out of 647 positive serum samples, type-specific PCR fragments were seen in 424 sera, while 13 (3.1%) of serum samples were of untype-able genotype. In all age groups genotype 3a had emerged as a predominant genotype 397 (93.6%), followed by 1b 8 (1.9%), 3b 4 (0.9%), 1a 2 (0.5%), while no sample detected to have 2a, 2b, 5a, 6a and mixed genotypes. The highest prevalence of untype-ables were seen in 61-70 age group. Conclusion: Need of the hour is proper sequencing of untype-able genotypes via upgrading existing methodologies. It will not only help the clinicians in achieving sustained virological response but also help in identifying new genotypes/subtypes.
format	article
author	Qanita Fahim Irfan Ali Mirza Ayesha Khalid Anum Imtiaz Asad Ahmad
author_facet	Qanita Fahim Irfan Ali Mirza Ayesha Khalid Anum Imtiaz Asad Ahmad
author_sort	Qanita Fahim
title	THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS
title_short	THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS
title_full	THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS
title_fullStr	THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS
title_full_unstemmed	THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS
title_sort	hepatitis c untype-able genotypes, an emergence of quasi-species in hcv infected patients
publisher	Army Medical College Rawalpindi
publishDate	2021
url	https://doi.org/10.51253/pafmj.v71iSuppl-1.2603 https://doaj.org/article/1c0eb1bfb9dd426c98769c26ec8758c2
work_keys_str_mv	AT qanitafahim thehepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT irfanalimirza thehepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT ayeshakhalid thehepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT anumimtiaz thehepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT asadahmad thehepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT qanitafahim hepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT irfanalimirza hepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT ayeshakhalid hepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT anumimtiaz hepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients AT asadahmad hepatitiscuntypeablegenotypesanemergenceofquasispeciesinhcvinfectedpatients
_version_	1718389002217717760

THE HEPATITIS C UNTYPE-ABLE GENOTYPES, AN EMERGENCE OF QUASI-SPECIES IN HCV INFECTED PATIENTS

Ejemplares similares