Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector

Ebrahim Azizi1,4, Alireza Namazi1, Ismaeil Haririan2,5, Shamileh Fouladdel1, Mohammad R Khoshayand3, Parisa Y Shotorbani6, Alireza Nomani1,7, Taraneh Gazori1,21Molecular Research Lab, Department of Pharmacology and Toxicology, 2Department of Pharmaceutics, 3Department of Food and Drug Control, Facul...

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Autores principales:	Ebrahim Azizi, Alireza Namazi, Ismaeil Haririan, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1c0fcb5b621149f1b0725e1b61c7f8a4
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spelling	oai:doaj.org-article:1c0fcb5b621149f1b0725e1b61c7f8a42021-12-02T07:37:41ZRelease profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector1176-91141178-2013https://doaj.org/article/1c0fcb5b621149f1b0725e1b61c7f8a42010-06-01T00:00:00Zhttp://www.dovepress.com/release-profile-and-stability-evaluation-of-optimized-chitosanalginate-a4698https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ebrahim Azizi1,4, Alireza Namazi1, Ismaeil Haririan2,5, Shamileh Fouladdel1, Mohammad R Khoshayand3, Parisa Y Shotorbani6, Alireza Nomani1,7, Taraneh Gazori1,21Molecular Research Lab, Department of Pharmacology and Toxicology, 2Department of Pharmaceutics, 3Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran; 5Biomaterials Research Center (BRC) Tehran, Iran; 6Pharmaceutical Sciences Branch, Azad University, Tehran, Iran; 7Department of Pharmaceutics, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, IranAbstract: Chitosan/alginate nanoparticles which had been optimized in our previous study using two different N/P ratios were chosen and their ability to release epidermal growth factor receptor (EGFR) antisense was investigated. In addition, the stability of these nanoparticles in aqueous medium and after freeze-drying was investigated. In the case of both N/P ratios (5, 25), nanoparticles started releasing EGFR antisense as soon as they were exposed to the medium and the release lasted for approximately 50 hours. Nanoparticle size, shape, zeta potential, and release profile did not show any significant change after the freeze-drying process (followed by reswelling). The nanoparticles were reswellable again after freeze-drying in phosphate buffer with a pH of 7.4 over a period of six hours. Agarose gel electrophoresis of the nanoparticles with the two different N/P ratios showed that these nanoparticles could protect EGFR antisense molecules for six hours.Keywords: chitosan/alginate nanoparticles, release profile, freeze-drying, agarose gel electrophoresis Ebrahim AziziAlireza NamaziIsmaeil Haririanet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 455-461 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ebrahim Azizi Alireza Namazi Ismaeil Haririan et al Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector
description	Ebrahim Azizi1,4, Alireza Namazi1, Ismaeil Haririan2,5, Shamileh Fouladdel1, Mohammad R Khoshayand3, Parisa Y Shotorbani6, Alireza Nomani1,7, Taraneh Gazori1,21Molecular Research Lab, Department of Pharmacology and Toxicology, 2Department of Pharmaceutics, 3Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran; 5Biomaterials Research Center (BRC) Tehran, Iran; 6Pharmaceutical Sciences Branch, Azad University, Tehran, Iran; 7Department of Pharmaceutics, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, IranAbstract: Chitosan/alginate nanoparticles which had been optimized in our previous study using two different N/P ratios were chosen and their ability to release epidermal growth factor receptor (EGFR) antisense was investigated. In addition, the stability of these nanoparticles in aqueous medium and after freeze-drying was investigated. In the case of both N/P ratios (5, 25), nanoparticles started releasing EGFR antisense as soon as they were exposed to the medium and the release lasted for approximately 50 hours. Nanoparticle size, shape, zeta potential, and release profile did not show any significant change after the freeze-drying process (followed by reswelling). The nanoparticles were reswellable again after freeze-drying in phosphate buffer with a pH of 7.4 over a period of six hours. Agarose gel electrophoresis of the nanoparticles with the two different N/P ratios showed that these nanoparticles could protect EGFR antisense molecules for six hours.Keywords: chitosan/alginate nanoparticles, release profile, freeze-drying, agarose gel electrophoresis
format	article
author	Ebrahim Azizi Alireza Namazi Ismaeil Haririan et al
author_facet	Ebrahim Azizi Alireza Namazi Ismaeil Haririan et al
author_sort	Ebrahim Azizi
title	Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector
title_short	Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector
title_full	Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector
title_fullStr	Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector
title_full_unstemmed	Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector
title_sort	release profile and stability evaluation of optimized chitosan/alginate nanoparticles as egfr antisense vector
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/1c0fcb5b621149f1b0725e1b61c7f8a4
work_keys_str_mv	AT ebrahimazizi releaseprofileandstabilityevaluationofoptimizedchitosanalginatenanoparticlesasegfrantisensevector AT alirezanamazi releaseprofileandstabilityevaluationofoptimizedchitosanalginatenanoparticlesasegfrantisensevector AT ismaeilharirian releaseprofileandstabilityevaluationofoptimizedchitosanalginatenanoparticlesasegfrantisensevector AT etal releaseprofileandstabilityevaluationofoptimizedchitosanalginatenanoparticlesasegfrantisensevector
_version_	1718399265791803392

Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector

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