Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p

Cleft lip with or without cleft palate (CL/P) is one of the most common congenital birth defects. This study aims to identify novel pathogenic microRNAs associated with cleft palate (CP). Through data analyses of miRNA-sequencing for developing palatal shelves of C57BL/6J mice, we found that miR-449...

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Autores principales:	Hiroki Yoshioka, Goo Jun, Akiko Suzuki, Junichi Iwata
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	environmental factor cleft palate microRNA gene regulation cell proliferation birth defect Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1c1444a23dcb476fa251f20619bf1989
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spelling	oai:doaj.org-article:1c1444a23dcb476fa251f20619bf19892021-11-25T17:56:49ZDexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p10.3390/ijms2222124531422-00671661-6596https://doaj.org/article/1c1444a23dcb476fa251f20619bf19892021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12453https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cleft lip with or without cleft palate (CL/P) is one of the most common congenital birth defects. This study aims to identify novel pathogenic microRNAs associated with cleft palate (CP). Through data analyses of miRNA-sequencing for developing palatal shelves of C57BL/6J mice, we found that miR-449a-3p, miR-449a-5p, miR-449b, miR-449c-3p, and miR-449c-5p were significantly upregulated, and that miR-19a-3p, miR-130a-3p, miR-301a-3p, and miR-486b-5p were significantly downregulated, at embryonic day E14.5 compared to E13.5. Among them, overexpression of the miR-449 family (miR-449a-3p, miR-449a-5p, miR-449b, miR-449c-3p, and miR-449c-5p) and miR-486b-5p resulted in reduced cell proliferation in primary mouse embryonic palatal mesenchymal (MEPM) cells and mouse cranial neural crest cell line O9-1. On the other hand, inhibitors of miR-130a-3p and miR-301a-3p significantly reduced cell proliferation in MEPM and O9-1 cells. Notably, we found that treatment with dexamethasone, a glucocorticoid known to induce CP in mice, suppressed miR-130a-3p expression in both MEPM and O9-1 cells. Moreover, a miR-130a-3p mimic could ameliorate the cell proliferation defect induced by dexamethasone through normalization of <i>Slc24a2</i> expression. Taken together, our results suggest that miR-130-3p plays a crucial role in dexamethasone-induced CP in mice.Hiroki YoshiokaGoo JunAkiko SuzukiJunichi IwataMDPI AGarticleenvironmental factorcleft palatemicroRNAgene regulationcell proliferationbirth defectBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12453, p 12453 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	environmental factor cleft palate microRNA gene regulation cell proliferation birth defect Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	environmental factor cleft palate microRNA gene regulation cell proliferation birth defect Biology (General) QH301-705.5 Chemistry QD1-999 Hiroki Yoshioka Goo Jun Akiko Suzuki Junichi Iwata Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p
description	Cleft lip with or without cleft palate (CL/P) is one of the most common congenital birth defects. This study aims to identify novel pathogenic microRNAs associated with cleft palate (CP). Through data analyses of miRNA-sequencing for developing palatal shelves of C57BL/6J mice, we found that miR-449a-3p, miR-449a-5p, miR-449b, miR-449c-3p, and miR-449c-5p were significantly upregulated, and that miR-19a-3p, miR-130a-3p, miR-301a-3p, and miR-486b-5p were significantly downregulated, at embryonic day E14.5 compared to E13.5. Among them, overexpression of the miR-449 family (miR-449a-3p, miR-449a-5p, miR-449b, miR-449c-3p, and miR-449c-5p) and miR-486b-5p resulted in reduced cell proliferation in primary mouse embryonic palatal mesenchymal (MEPM) cells and mouse cranial neural crest cell line O9-1. On the other hand, inhibitors of miR-130a-3p and miR-301a-3p significantly reduced cell proliferation in MEPM and O9-1 cells. Notably, we found that treatment with dexamethasone, a glucocorticoid known to induce CP in mice, suppressed miR-130a-3p expression in both MEPM and O9-1 cells. Moreover, a miR-130a-3p mimic could ameliorate the cell proliferation defect induced by dexamethasone through normalization of <i>Slc24a2</i> expression. Taken together, our results suggest that miR-130-3p plays a crucial role in dexamethasone-induced CP in mice.
format	article
author	Hiroki Yoshioka Goo Jun Akiko Suzuki Junichi Iwata
author_facet	Hiroki Yoshioka Goo Jun Akiko Suzuki Junichi Iwata
author_sort	Hiroki Yoshioka
title	Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p
title_short	Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p
title_full	Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p
title_fullStr	Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p
title_full_unstemmed	Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p
title_sort	dexamethasone suppresses palatal cell proliferation through mir-130a-3p
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1c1444a23dcb476fa251f20619bf1989
work_keys_str_mv	AT hirokiyoshioka dexamethasonesuppressespalatalcellproliferationthroughmir130a3p AT goojun dexamethasonesuppressespalatalcellproliferationthroughmir130a3p AT akikosuzuki dexamethasonesuppressespalatalcellproliferationthroughmir130a3p AT junichiiwata dexamethasonesuppressespalatalcellproliferationthroughmir130a3p
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Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p

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