Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress

Yong Zhu,1,* Guoying Deng,2,* Anqi Ji,2 Jiayi Yao,1 Xiaoxiao Meng,1 Jinfeng Wang,1 Qian Wang,2 Qiugen Wang,2 Ruilan Wang1 1Department of Critical Care Medicine, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine, 2Trauma Center, Shanghai General Hospital, Shanghai Jiaotong...

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Autores principales:	Zhu Y, Deng G, Ji A, Yao J, Meng X, Wang J, Wang Q, Wang R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	porous Se@SiO2 nanospheres acute lung injury paraquat poisoning oxidative stress inflammatory cytokines Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1c1708d2de1c4fef8a2682fd526c9b71
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spelling	oai:doaj.org-article:1c1708d2de1c4fef8a2682fd526c9b712021-12-02T04:28:18ZPorous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress1178-2013https://doaj.org/article/1c1708d2de1c4fef8a2682fd526c9b712017-09-01T00:00:00Zhttps://www.dovepress.com/porous-sesio2-nanospheres-treated-paraquat-induced-acute-lung-injury-b-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yong Zhu,1,* Guoying Deng,2,* Anqi Ji,2 Jiayi Yao,1 Xiaoxiao Meng,1 Jinfeng Wang,1 Qian Wang,2 Qiugen Wang,2 Ruilan Wang1 1Department of Critical Care Medicine, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine, 2Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China *These authors contributed equally to this work Abstract: Acute paraquat (PQ) poisoning is one of the most common forms of pesticide poisoning. Oxidative stress and inflammation are thought to be important mechanisms in PQ-induced acute lung injury (ALI). Selenium (Se) can scavenge intracellular free radicals directly or indirectly. In this study, we investigated whether porous Se@SiO2 nanospheres could alleviate oxidative stress and inflammation in PQ-induced ALI. Male Sprague Dawley rats and RLE-6TN cells were used in this study. Rats were categorized into 3 groups: control (n=6), PQ (n=18), and PQ + Se@SiO2 (n=18). The PQ and PQ + Se@SiO2 groups were randomly and evenly divided into 3 sub-groups according to different time points (24, 48 and 72 h) after PQ treatment. Porous Se@SiO2 nanospheres 1 mg/kg (in the PQ + Se@SiO2 group) were administered via intraperitoneal injection every 24 h. Expression levels of reduced glutathione, malondialdehyde, superoxide dismutase, reactive oxygen species (ROS), nuclear factor-κB (NF-κB), phosphorylated NF-κB (p-NF-κB), tumor necrosis factor-α and interleukin-1β were detected, and a histological analysis of rat lung tissues was performed. The results showed that the levels of ROS, malondialdehyde, NF-κB, p-NF-κB, tumor necrosis factor-α and interleukin-1β were markedly increased after PQ treatment. Glutathione and superoxide dismutase levels were reduced. However, treatment with porous Se@SiO2 nanospheres markedly alleviated PQ-induced oxidative stress and inflammation. Additionally, the results from histological examinations and wet-to-dry weight ratios of rat lung tissues showed that lung damage was reduced after porous Se@SiO2 nanosphere treatment. These data indicate that porous Se@SiO2 nanospheres may reduce NF-κB, p-NF-κB and inflammatory cytokine levels by inhibiting ROS in PQ-induced ALI. This study demonstrates that porous Se@SiO2 nanospheres may be a therapeutic method for use in the future for PQ poisoning. Keywords: porous Se@SiO2 nanospheres, acute lung injury, paraquat poisoning, oxidative stress, inflammatory cytokines, ROS, NF-kappa BZhu YDeng GJi AYao JMeng XWang JWang QWang QWang RDove Medical Pressarticleporous Se@SiO2 nanospheresacute lung injuryparaquat poisoningoxidative stressinflammatory cytokinesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7143-7152 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	porous Se@SiO2 nanospheres acute lung injury paraquat poisoning oxidative stress inflammatory cytokines Medicine (General) R5-920
spellingShingle	porous Se@SiO2 nanospheres acute lung injury paraquat poisoning oxidative stress inflammatory cytokines Medicine (General) R5-920 Zhu Y Deng G Ji A Yao J Meng X Wang J Wang Q Wang Q Wang R Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress
description	Yong Zhu,1,* Guoying Deng,2,* Anqi Ji,2 Jiayi Yao,1 Xiaoxiao Meng,1 Jinfeng Wang,1 Qian Wang,2 Qiugen Wang,2 Ruilan Wang1 1Department of Critical Care Medicine, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine, 2Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China *These authors contributed equally to this work Abstract: Acute paraquat (PQ) poisoning is one of the most common forms of pesticide poisoning. Oxidative stress and inflammation are thought to be important mechanisms in PQ-induced acute lung injury (ALI). Selenium (Se) can scavenge intracellular free radicals directly or indirectly. In this study, we investigated whether porous Se@SiO2 nanospheres could alleviate oxidative stress and inflammation in PQ-induced ALI. Male Sprague Dawley rats and RLE-6TN cells were used in this study. Rats were categorized into 3 groups: control (n=6), PQ (n=18), and PQ + Se@SiO2 (n=18). The PQ and PQ + Se@SiO2 groups were randomly and evenly divided into 3 sub-groups according to different time points (24, 48 and 72 h) after PQ treatment. Porous Se@SiO2 nanospheres 1 mg/kg (in the PQ + Se@SiO2 group) were administered via intraperitoneal injection every 24 h. Expression levels of reduced glutathione, malondialdehyde, superoxide dismutase, reactive oxygen species (ROS), nuclear factor-κB (NF-κB), phosphorylated NF-κB (p-NF-κB), tumor necrosis factor-α and interleukin-1β were detected, and a histological analysis of rat lung tissues was performed. The results showed that the levels of ROS, malondialdehyde, NF-κB, p-NF-κB, tumor necrosis factor-α and interleukin-1β were markedly increased after PQ treatment. Glutathione and superoxide dismutase levels were reduced. However, treatment with porous Se@SiO2 nanospheres markedly alleviated PQ-induced oxidative stress and inflammation. Additionally, the results from histological examinations and wet-to-dry weight ratios of rat lung tissues showed that lung damage was reduced after porous Se@SiO2 nanosphere treatment. These data indicate that porous Se@SiO2 nanospheres may reduce NF-κB, p-NF-κB and inflammatory cytokine levels by inhibiting ROS in PQ-induced ALI. This study demonstrates that porous Se@SiO2 nanospheres may be a therapeutic method for use in the future for PQ poisoning. Keywords: porous Se@SiO2 nanospheres, acute lung injury, paraquat poisoning, oxidative stress, inflammatory cytokines, ROS, NF-kappa B
format	article
author	Zhu Y Deng G Ji A Yao J Meng X Wang J Wang Q Wang Q Wang R
author_facet	Zhu Y Deng G Ji A Yao J Meng X Wang J Wang Q Wang Q Wang R
author_sort	Zhu Y
title	Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress
title_short	Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress
title_full	Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress
title_fullStr	Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress
title_full_unstemmed	Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress
title_sort	porous se@sio2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/1c1708d2de1c4fef8a2682fd526c9b71
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Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress

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