Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model

Ji Young Kang, Sook Young Lee, Chin Kook Rhee, Seung Joon Kim, Soon Seog Kwon, Young Kyoon KimDepartment of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, KoreaBackground and objectives: The influence of aging on the development of asthma has not been studied thoroughly...

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Autores principales:	Kang JY, Lee SY, Rhee CK, Kim SJ, Kwon SS, Kim YK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	aging asthma airway remodeling muscarinic receptor Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/1c22a8c8da9f4c4d9a94725214d0a0d6
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spelling	oai:doaj.org-article:1c22a8c8da9f4c4d9a94725214d0a0d62021-12-02T08:00:03ZEffect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model1178-1998https://doaj.org/article/1c22a8c8da9f4c4d9a94725214d0a0d62013-10-01T00:00:00Zhttps://www.dovepress.com/effect-of-aging-on-airway-remodeling-and-muscarinic-receptors-in-a-mur-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Ji Young Kang, Sook Young Lee, Chin Kook Rhee, Seung Joon Kim, Soon Seog Kwon, Young Kyoon KimDepartment of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, KoreaBackground and objectives: The influence of aging on the development of asthma has not been studied thoroughly. The aim of this study was to investigate age-related airway responses involving lung histology and expression of muscarinic receptors in a murine model of acute asthma. Methods: Female BALB/c mice at the ages of 6 weeks and 6, 9, and 12 months were sensitized and challenged with ovalbumin (OVA) for 1 month (n = 8–12 per group). We analyzed inflammatory cells and T-helper (Th)2 cytokines in bronchoalveolar lavage (BAL) fluid and parameters of airway remodeling and expression of muscarinic receptors in lung tissue. Results: Among the OVA groups, total cell and eosinophil numbers in BAL fluid were significantly higher in the older (6-, 9-, and 12-month-old) mice than in the young (6-week-old) mice. Interleukin (IL) 4 (IL-4) concentration increased, but IL-5 and IL-13 concentrations showed a decreased tendency, with age. IL-17 concentration tended to increase with age, which did not reach statistical significance. periodic acid-Schiff (PAS) staining area, peribronchial collagen deposition, and area of α-smooth muscle staining were significantly higher in the 6-month older OVA group than in the young OVA group. The expression of the M3 and M2 muscarinic receptors tended to increase and decrease, respectively, with age. Conclusion: The aged mice showed an active and unique pattern not only on airway inflammation, but also on airway remodeling and expression of the muscarinic receptors during the development of acute asthma compared with the young mice. These findings suggest that the aging process affects the pathogenesis of acute asthma and age-specific approach might be more appropriate for better asthma control in a clinical practice.Keywords: aging, asthma, airway remodeling, muscarinic receptorKang JYLee SYRhee CKKim SJKwon SSKim YKDove Medical Pressarticleagingasthmaairway remodelingmuscarinic receptorGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 8, Pp 1393-1403 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	aging asthma airway remodeling muscarinic receptor Geriatrics RC952-954.6
spellingShingle	aging asthma airway remodeling muscarinic receptor Geriatrics RC952-954.6 Kang JY Lee SY Rhee CK Kim SJ Kwon SS Kim YK Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model
description	Ji Young Kang, Sook Young Lee, Chin Kook Rhee, Seung Joon Kim, Soon Seog Kwon, Young Kyoon KimDepartment of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, KoreaBackground and objectives: The influence of aging on the development of asthma has not been studied thoroughly. The aim of this study was to investigate age-related airway responses involving lung histology and expression of muscarinic receptors in a murine model of acute asthma. Methods: Female BALB/c mice at the ages of 6 weeks and 6, 9, and 12 months were sensitized and challenged with ovalbumin (OVA) for 1 month (n = 8–12 per group). We analyzed inflammatory cells and T-helper (Th)2 cytokines in bronchoalveolar lavage (BAL) fluid and parameters of airway remodeling and expression of muscarinic receptors in lung tissue. Results: Among the OVA groups, total cell and eosinophil numbers in BAL fluid were significantly higher in the older (6-, 9-, and 12-month-old) mice than in the young (6-week-old) mice. Interleukin (IL) 4 (IL-4) concentration increased, but IL-5 and IL-13 concentrations showed a decreased tendency, with age. IL-17 concentration tended to increase with age, which did not reach statistical significance. periodic acid-Schiff (PAS) staining area, peribronchial collagen deposition, and area of α-smooth muscle staining were significantly higher in the 6-month older OVA group than in the young OVA group. The expression of the M3 and M2 muscarinic receptors tended to increase and decrease, respectively, with age. Conclusion: The aged mice showed an active and unique pattern not only on airway inflammation, but also on airway remodeling and expression of the muscarinic receptors during the development of acute asthma compared with the young mice. These findings suggest that the aging process affects the pathogenesis of acute asthma and age-specific approach might be more appropriate for better asthma control in a clinical practice.Keywords: aging, asthma, airway remodeling, muscarinic receptor
format	article
author	Kang JY Lee SY Rhee CK Kim SJ Kwon SS Kim YK
author_facet	Kang JY Lee SY Rhee CK Kim SJ Kwon SS Kim YK
author_sort	Kang JY
title	Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model
title_short	Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model
title_full	Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model
title_fullStr	Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model
title_full_unstemmed	Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model
title_sort	effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/1c22a8c8da9f4c4d9a94725214d0a0d6
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Effect of aging on airway remodeling and muscarinic receptors in a murine acute asthma model

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