Diaphragm muscle weakness in an experimental porcine intensive care unit model.

In critically ill patients, mechanisms underlying diaphragm muscle remodeling and resultant dysfunction contributing to weaning failure remain unclear. Ventilator-induced modifications as well as sepsis and administration of pharmacological agents such as corticosteroids and neuromuscular blocking a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Julien Ochala, Guillaume Renaud, Monica Llano Diez, Varuna C Banduseela, Sudhakar Aare, Karsten Ahlbeck, Peter J Radell, Lars I Eriksson, Lars Larsson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c30db448b66402697f4458d015a287a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1c30db448b66402697f4458d015a287a
record_format	dspace
spelling	oai:doaj.org-article:1c30db448b66402697f4458d015a287a2021-11-18T06:52:00ZDiaphragm muscle weakness in an experimental porcine intensive care unit model.1932-620310.1371/journal.pone.0020558https://doaj.org/article/1c30db448b66402697f4458d015a287a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21698290/?tool=EBIhttps://doaj.org/toc/1932-6203In critically ill patients, mechanisms underlying diaphragm muscle remodeling and resultant dysfunction contributing to weaning failure remain unclear. Ventilator-induced modifications as well as sepsis and administration of pharmacological agents such as corticosteroids and neuromuscular blocking agents may be involved. Thus, the objective of the present study was to examine how sepsis, systemic corticosteroid treatment (CS) and neuromuscular blocking agent administration (NMBA) aggravate ventilator-related diaphragm cell and molecular dysfunction in the intensive care unit. Piglets were exposed to different combinations of mechanical ventilation and sedation, endotoxin-induced sepsis, CS and NMBA for five days and compared with sham-operated control animals. On day 5, diaphragm muscle fibre structure (myosin heavy chain isoform proportion, cross-sectional area and contractile protein content) did not differ from controls in any of the mechanically ventilated animals. However, a decrease in single fibre maximal force normalized to cross-sectional area (specific force) was observed in all experimental piglets. Therefore, exposure to mechanical ventilation and sedation for five days has a key negative impact on diaphragm contractile function despite a preservation of muscle structure. Post-translational modifications of contractile proteins are forwarded as one probable underlying mechanism. Unexpectedly, sepsis, CS or NMBA have no significant additive effects, suggesting that mechanical ventilation and sedation are the triggering factors leading to diaphragm weakness in the intensive care unit.Julien OchalaGuillaume RenaudMonica Llano DiezVaruna C BanduseelaSudhakar AareKarsten AhlbeckPeter J RadellLars I ErikssonLars LarssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e20558 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julien Ochala Guillaume Renaud Monica Llano Diez Varuna C Banduseela Sudhakar Aare Karsten Ahlbeck Peter J Radell Lars I Eriksson Lars Larsson Diaphragm muscle weakness in an experimental porcine intensive care unit model.
description	In critically ill patients, mechanisms underlying diaphragm muscle remodeling and resultant dysfunction contributing to weaning failure remain unclear. Ventilator-induced modifications as well as sepsis and administration of pharmacological agents such as corticosteroids and neuromuscular blocking agents may be involved. Thus, the objective of the present study was to examine how sepsis, systemic corticosteroid treatment (CS) and neuromuscular blocking agent administration (NMBA) aggravate ventilator-related diaphragm cell and molecular dysfunction in the intensive care unit. Piglets were exposed to different combinations of mechanical ventilation and sedation, endotoxin-induced sepsis, CS and NMBA for five days and compared with sham-operated control animals. On day 5, diaphragm muscle fibre structure (myosin heavy chain isoform proportion, cross-sectional area and contractile protein content) did not differ from controls in any of the mechanically ventilated animals. However, a decrease in single fibre maximal force normalized to cross-sectional area (specific force) was observed in all experimental piglets. Therefore, exposure to mechanical ventilation and sedation for five days has a key negative impact on diaphragm contractile function despite a preservation of muscle structure. Post-translational modifications of contractile proteins are forwarded as one probable underlying mechanism. Unexpectedly, sepsis, CS or NMBA have no significant additive effects, suggesting that mechanical ventilation and sedation are the triggering factors leading to diaphragm weakness in the intensive care unit.
format	article
author	Julien Ochala Guillaume Renaud Monica Llano Diez Varuna C Banduseela Sudhakar Aare Karsten Ahlbeck Peter J Radell Lars I Eriksson Lars Larsson
author_facet	Julien Ochala Guillaume Renaud Monica Llano Diez Varuna C Banduseela Sudhakar Aare Karsten Ahlbeck Peter J Radell Lars I Eriksson Lars Larsson
author_sort	Julien Ochala
title	Diaphragm muscle weakness in an experimental porcine intensive care unit model.
title_short	Diaphragm muscle weakness in an experimental porcine intensive care unit model.
title_full	Diaphragm muscle weakness in an experimental porcine intensive care unit model.
title_fullStr	Diaphragm muscle weakness in an experimental porcine intensive care unit model.
title_full_unstemmed	Diaphragm muscle weakness in an experimental porcine intensive care unit model.
title_sort	diaphragm muscle weakness in an experimental porcine intensive care unit model.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1c30db448b66402697f4458d015a287a
work_keys_str_mv	AT julienochala diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT guillaumerenaud diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT monicallanodiez diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT varunacbanduseela diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT sudhakaraare diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT karstenahlbeck diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT peterjradell diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT larsieriksson diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel AT larslarsson diaphragmmuscleweaknessinanexperimentalporcineintensivecareunitmodel
_version_	1718424275986153472

Diaphragm muscle weakness in an experimental porcine intensive care unit model.

Ejemplares similares