Dystrophin in the Neonatal and Adult Rat Intestine

Background: Gastrointestinal (GI) complaints are frequently noted in aging dystrophinopathy patients, yet their underlying molecular mechanisms are largely unknown. As dystrophin protein isoform 71 (Dp71) is particularly implicated in the development of smooth muscle cells, we evaluated its distribu...

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Autores principales:	Judith M. Lionarons, Govert Hoogland, Rutger J. Slegers, Hellen Steinbusch, Sandra M. H. Claessen, Johan S. H. Vles
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dystrophinopathy aging peripheral nervous system rat intestine development Science Q
Acceso en línea:	https://doaj.org/article/1c3819d046fa4aecaafbe6c47f2824c4
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spelling	oai:doaj.org-article:1c3819d046fa4aecaafbe6c47f2824c42021-11-25T18:10:44ZDystrophin in the Neonatal and Adult Rat Intestine10.3390/life111111552075-1729https://doaj.org/article/1c3819d046fa4aecaafbe6c47f2824c42021-10-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1155https://doaj.org/toc/2075-1729Background: Gastrointestinal (GI) complaints are frequently noted in aging dystrophinopathy patients, yet their underlying molecular mechanisms are largely unknown. As dystrophin protein isoform 71 (Dp71) is particularly implicated in the development of smooth muscle cells, we evaluated its distribution in the neonatal and adult rat intestine in this study. Methods: Dp71 expression levels were assessed in the proximal (duodenum, jejunum and ileum) and distal (caecum, colon and rectum) intestine by Western blotting and qPCR. In addition, the cellular distribution of total Dp was evaluated in the duodenum and colon by immunohistochemical colocalization studies with alpha-smooth muscle actin (aSMA), Hu RNA binding proteins C and D (HuC/HuD) for neurons and vimentin (VIM) for interstitial cells. Results: In neonatal and adult rats, the distal intestine expressed 2.5 times more Dp71 protein than the proximal part (<i>p</i> < 0.01). This regional difference was not observed in Dp71 mRNA. During both stages, Dp-immunoreactivity was predominant in the muscularis propria, where it co-localized with aSMA and HuC/HuD. Conclusions: In neonatal and adult rats, Dp71 was expressed highest in the distal intestine. Together with the observation that Dp may be expressed by myenteric neurons, this warrants a paradigm shift in the treatment of GI comorbidities.Judith M. LionaronsGovert HooglandRutger J. SlegersHellen SteinbuschSandra M. H. ClaessenJohan S. H. VlesMDPI AGarticledystrophinopathyagingperipheral nervous systemratintestinedevelopmentScienceQENLife, Vol 11, Iss 1155, p 1155 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dystrophinopathy aging peripheral nervous system rat intestine development Science Q
spellingShingle	dystrophinopathy aging peripheral nervous system rat intestine development Science Q Judith M. Lionarons Govert Hoogland Rutger J. Slegers Hellen Steinbusch Sandra M. H. Claessen Johan S. H. Vles Dystrophin in the Neonatal and Adult Rat Intestine
description	Background: Gastrointestinal (GI) complaints are frequently noted in aging dystrophinopathy patients, yet their underlying molecular mechanisms are largely unknown. As dystrophin protein isoform 71 (Dp71) is particularly implicated in the development of smooth muscle cells, we evaluated its distribution in the neonatal and adult rat intestine in this study. Methods: Dp71 expression levels were assessed in the proximal (duodenum, jejunum and ileum) and distal (caecum, colon and rectum) intestine by Western blotting and qPCR. In addition, the cellular distribution of total Dp was evaluated in the duodenum and colon by immunohistochemical colocalization studies with alpha-smooth muscle actin (aSMA), Hu RNA binding proteins C and D (HuC/HuD) for neurons and vimentin (VIM) for interstitial cells. Results: In neonatal and adult rats, the distal intestine expressed 2.5 times more Dp71 protein than the proximal part (<i>p</i> < 0.01). This regional difference was not observed in Dp71 mRNA. During both stages, Dp-immunoreactivity was predominant in the muscularis propria, where it co-localized with aSMA and HuC/HuD. Conclusions: In neonatal and adult rats, Dp71 was expressed highest in the distal intestine. Together with the observation that Dp may be expressed by myenteric neurons, this warrants a paradigm shift in the treatment of GI comorbidities.
format	article
author	Judith M. Lionarons Govert Hoogland Rutger J. Slegers Hellen Steinbusch Sandra M. H. Claessen Johan S. H. Vles
author_facet	Judith M. Lionarons Govert Hoogland Rutger J. Slegers Hellen Steinbusch Sandra M. H. Claessen Johan S. H. Vles
author_sort	Judith M. Lionarons
title	Dystrophin in the Neonatal and Adult Rat Intestine
title_short	Dystrophin in the Neonatal and Adult Rat Intestine
title_full	Dystrophin in the Neonatal and Adult Rat Intestine
title_fullStr	Dystrophin in the Neonatal and Adult Rat Intestine
title_full_unstemmed	Dystrophin in the Neonatal and Adult Rat Intestine
title_sort	dystrophin in the neonatal and adult rat intestine
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1c3819d046fa4aecaafbe6c47f2824c4
work_keys_str_mv	AT judithmlionarons dystrophinintheneonatalandadultratintestine AT goverthoogland dystrophinintheneonatalandadultratintestine AT rutgerjslegers dystrophinintheneonatalandadultratintestine AT hellensteinbusch dystrophinintheneonatalandadultratintestine AT sandramhclaessen dystrophinintheneonatalandadultratintestine AT johanshvles dystrophinintheneonatalandadultratintestine
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Dystrophin in the Neonatal and Adult Rat Intestine

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