Novel Observations Concerning Differentiation of Bloodstream-Form Trypanosomes to the Form That Is Adapted for Growth in Tsetse Flies
ABSTRACT Salivarian trypanosomes grow in mammals, where they depend on glucose, and as procyclic forms in tsetse flies, where they metabolize proline. Differentiation of bloodstream forms to nongrowing stumpy forms, and to procyclic forms, has been studied extensively, but reconciling the results is...
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
American Society for Microbiology
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/1c3bceb95ce04558bd8e0b917b62c027 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | ABSTRACT Salivarian trypanosomes grow in mammals, where they depend on glucose, and as procyclic forms in tsetse flies, where they metabolize proline. Differentiation of bloodstream forms to nongrowing stumpy forms, and to procyclic forms, has been studied extensively, but reconciling the results is tricky because investigators have used parasites with various differentiation competences and different media for procyclic-form culture. Standard protocols include lowering the temperature to 27°C, adding a tricarboxylic acid, and transferring the parasites to high-proline medium, often including glucose. A 20°C cold shock enhanced efficiency. Y. Qiu, J. E. Milanes, J. A. Jones, R. E. Noorai, et al. (mSphere 3:e00366-18, 2018, https://doi.org/10.1128/mSphere.00366-18) studied this systematically, and their results call long-established protocols into question. Importantly, highly efficient differentiation was observed after cold shock and transfer to no-glucose medium without tricarboxylic acid; in contrast, glucose made differentiation tricarboxylic acid dependent and inhibited procyclic growth. New transcriptome data for stumpy and procyclic forms will enable informative comparisons with biochemical observations and with other RNA and protein data sets. |
|---|