CRISPR/Cpf1-mediated DNA-free plant genome editing

Cpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in...

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Autores principales:	Hyeran Kim, Sang-Tae Kim, Jahee Ryu, Beum-Chang Kang, Jin-Soo Kim, Sang-Gyu Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1c3f50a8ce6e434eb8bae032f341edf5
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spelling	oai:doaj.org-article:1c3f50a8ce6e434eb8bae032f341edf52021-12-02T14:42:19ZCRISPR/Cpf1-mediated DNA-free plant genome editing10.1038/ncomms144062041-1723https://doaj.org/article/1c3f50a8ce6e434eb8bae032f341edf52017-02-01T00:00:00Zhttps://doi.org/10.1038/ncomms14406https://doaj.org/toc/2041-1723Cpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in plants.Hyeran KimSang-Tae KimJahee RyuBeum-Chang KangJin-Soo KimSang-Gyu KimNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hyeran Kim Sang-Tae Kim Jahee Ryu Beum-Chang Kang Jin-Soo Kim Sang-Gyu Kim CRISPR/Cpf1-mediated DNA-free plant genome editing
description	Cpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in plants.
format	article
author	Hyeran Kim Sang-Tae Kim Jahee Ryu Beum-Chang Kang Jin-Soo Kim Sang-Gyu Kim
author_facet	Hyeran Kim Sang-Tae Kim Jahee Ryu Beum-Chang Kang Jin-Soo Kim Sang-Gyu Kim
author_sort	Hyeran Kim
title	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_short	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_full	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_fullStr	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_full_unstemmed	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_sort	crispr/cpf1-mediated dna-free plant genome editing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1c3f50a8ce6e434eb8bae032f341edf5
work_keys_str_mv	AT hyerankim crisprcpf1mediateddnafreeplantgenomeediting AT sangtaekim crisprcpf1mediateddnafreeplantgenomeediting AT jaheeryu crisprcpf1mediateddnafreeplantgenomeediting AT beumchangkang crisprcpf1mediateddnafreeplantgenomeediting AT jinsookim crisprcpf1mediateddnafreeplantgenomeediting AT sanggyukim crisprcpf1mediateddnafreeplantgenomeediting
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CRISPR/Cpf1-mediated DNA-free plant genome editing

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