The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways

Eunjoo Kim,1 Joon Mee Kim,2 Lucia Kim,2 Suk Jin Choi,2 In Suh Park,2 Jee Young Han,2 Young Chae Chu,2 Eun Sook Choi,1 Kun Na,3 Soon-Sun Hong4 1Division of Nano and Energy Convergence Research, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 2Department of Pathology, Inha Univers...

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Autores principales:	Kim E, Kim JM, Kim L, Choi SJ, Park IS, Han JY, Chu YC, Choi ES, Na K, Hong SS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	iron oxide nanoparticles neutral-hydrophobic surface signaling pathway uptake accumulation reactive oxygen species (ROS) Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1c447792529548b58ae5ec01bea4d02c
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spelling	oai:doaj.org-article:1c447792529548b58ae5ec01bea4d02c2021-12-02T05:10:42ZThe effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways1178-2013https://doaj.org/article/1c447792529548b58ae5ec01bea4d02c2016-09-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-neutral-surface-iron-oxide-nanoparticles-on-cellular-upt-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Eunjoo Kim,1 Joon Mee Kim,2 Lucia Kim,2 Suk Jin Choi,2 In Suh Park,2 Jee Young Han,2 Young Chae Chu,2 Eun Sook Choi,1 Kun Na,3 Soon-Sun Hong4 1Division of Nano and Energy Convergence Research, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 2Department of Pathology, Inha University College of Medicine, Incheon, 3Department of Biotechnology, Catholic University of Korea, Bucheon, 4Department of Biomedical Sciences, Inha University College of Medicine, Incheon, South Korea Abstract: In recent years, iron oxide nanoparticles (IONPs) have been applied widely to biomedical fields. However, the relationship between the physicochemical properties of IONPs and their biological behavior is not fully understood yet. We prepared 3-methacryloxypropyltrimethoxysilane (MPS)-coated IONPs, which have a neutral hydrophobic surface, and compared their biological behavior to that of Resovist (ferucarbotran), a commercialized IONP formulation modified with carboxymethyl dextran. The rate of MPS-IONP uptake by human aortic endothelial cells (HAoECs) was higher than ferucarbotran uptake, indicating that the neutral hydrophobic nature of MPS-IONPs allowed them to be absorbed more readily through the plasma membrane. However, the signaling pathways activated by MPS-IONPs and ferucarbotran were comparable, suggesting that surface charge is not a key factor for inducing changes in HAoECs. In vivo fate analysis showed that MPS-IONPs accumulated for longer periods in tissues than hydrophilic ferucarbotran. These findings could enlarge our understanding of NP behavior for advanced applications in the biomedical field. Keywords: iron oxide nanoparticles, neutral hydrophobic surface, signaling pathway, uptake, accumulation, reactive oxygen species (ROS)Kim EKim JMKim LChoi SJPark ISHan JYChu YCChoi ESNa KHong SSDove Medical Pressarticleiron oxide nanoparticlesneutral-hydrophobic surfacesignaling pathwayuptakeaccumulationreactive oxygen species (ROS)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4595-4607 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	iron oxide nanoparticles neutral-hydrophobic surface signaling pathway uptake accumulation reactive oxygen species (ROS) Medicine (General) R5-920
spellingShingle	iron oxide nanoparticles neutral-hydrophobic surface signaling pathway uptake accumulation reactive oxygen species (ROS) Medicine (General) R5-920 Kim E Kim JM Kim L Choi SJ Park IS Han JY Chu YC Choi ES Na K Hong SS The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways
description	Eunjoo Kim,1 Joon Mee Kim,2 Lucia Kim,2 Suk Jin Choi,2 In Suh Park,2 Jee Young Han,2 Young Chae Chu,2 Eun Sook Choi,1 Kun Na,3 Soon-Sun Hong4 1Division of Nano and Energy Convergence Research, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 2Department of Pathology, Inha University College of Medicine, Incheon, 3Department of Biotechnology, Catholic University of Korea, Bucheon, 4Department of Biomedical Sciences, Inha University College of Medicine, Incheon, South Korea Abstract: In recent years, iron oxide nanoparticles (IONPs) have been applied widely to biomedical fields. However, the relationship between the physicochemical properties of IONPs and their biological behavior is not fully understood yet. We prepared 3-methacryloxypropyltrimethoxysilane (MPS)-coated IONPs, which have a neutral hydrophobic surface, and compared their biological behavior to that of Resovist (ferucarbotran), a commercialized IONP formulation modified with carboxymethyl dextran. The rate of MPS-IONP uptake by human aortic endothelial cells (HAoECs) was higher than ferucarbotran uptake, indicating that the neutral hydrophobic nature of MPS-IONPs allowed them to be absorbed more readily through the plasma membrane. However, the signaling pathways activated by MPS-IONPs and ferucarbotran were comparable, suggesting that surface charge is not a key factor for inducing changes in HAoECs. In vivo fate analysis showed that MPS-IONPs accumulated for longer periods in tissues than hydrophilic ferucarbotran. These findings could enlarge our understanding of NP behavior for advanced applications in the biomedical field. Keywords: iron oxide nanoparticles, neutral hydrophobic surface, signaling pathway, uptake, accumulation, reactive oxygen species (ROS)
format	article
author	Kim E Kim JM Kim L Choi SJ Park IS Han JY Chu YC Choi ES Na K Hong SS
author_facet	Kim E Kim JM Kim L Choi SJ Park IS Han JY Chu YC Choi ES Na K Hong SS
author_sort	Kim E
title	The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways
title_short	The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways
title_full	The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways
title_fullStr	The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways
title_full_unstemmed	The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways
title_sort	effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/1c447792529548b58ae5ec01bea4d02c
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The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways

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