Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Abstract In this study, two highly thermotolerant and methanol-tolerant lipase-producing bacteria were isolated from cooking oil and they exhibited a high number of catalytic lipase activities recording 18.65 ± 0.68 U/mL and 13.14 ± 0.03 U/mL, respectively. Bacterial isolates were identified accordi...

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Autores principales:	Azhar Najjar, Elhagag Ahmed Hassan, Nidal Zabermawi, Saber H. Saber, Leena H. Bajrai, Mohammed S. Almuhayawi, Turki S. Abujamel, Saad B. Almasaudi, Leena E. Azhar, Mohammed Moulay, Steve Harakeh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c49380b4a8547e08d0a4ffd225faa7f
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spelling	oai:doaj.org-article:1c49380b4a8547e08d0a4ffd225faa7f2021-12-02T16:31:46ZOptimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes10.1038/s41598-021-93023-z2045-2322https://doaj.org/article/1c49380b4a8547e08d0a4ffd225faa7f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93023-zhttps://doaj.org/toc/2045-2322Abstract In this study, two highly thermotolerant and methanol-tolerant lipase-producing bacteria were isolated from cooking oil and they exhibited a high number of catalytic lipase activities recording 18.65 ± 0.68 U/mL and 13.14 ± 0.03 U/mL, respectively. Bacterial isolates were identified according to phenotypic and genotypic 16S rRNA characterization as Kocuria flava ASU5 (MT919305) and Bacillus circulans ASU11 (MT919306). Lipases produced from Kocuria flava ASU5 showed the highest methanol tolerance, recording 98.4% relative activity as well as exhibited high thermostability and alkaline stability. Under the optimum conditions obtained from 3D plots of response surface methodology design, the Kocuria flava ASU5 biocatalyst exhibited an 83.08% yield of biodiesel at optimized reaction variables of, 60 ○C, pH value 8 and 1:2 oil/alcohol molar ratios in the reaction mixture. As well as, the obtained results showed the interactions of temperature/methanol were significant effects, whereas this was not noted in the case of temperature/pH and pH/methanol interactions. The obtained amount of biodiesel from cooking oil was 83.08%, which was analyzed by a GC/Ms profile. The produced biodiesel was confirmed by Fourier-transform infrared spectroscopy (FTIR) approaches showing an absorption band at 1743 cm−1, which is recognized for its absorption in the carbonyl group (C=O) which is characteristic of ester absorption. The energy content generated from biodiesel synthesized was estimated as 12,628.5 kJ/mol. Consequently, Kocuria flava MT919305 may provide promising thermostable, methanol-tolerant lipases, which may improve the economic feasibility and biotechnology of enzyme biocatalysis in the synthesis of value-added green chemicals.Azhar NajjarElhagag Ahmed HassanNidal ZabermawiSaber H. SaberLeena H. BajraiMohammed S. AlmuhayawiTurki S. AbujamelSaad B. AlmasaudiLeena E. AzharMohammed MoulaySteve HarakehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Azhar Najjar Elhagag Ahmed Hassan Nidal Zabermawi Saber H. Saber Leena H. Bajrai Mohammed S. Almuhayawi Turki S. Abujamel Saad B. Almasaudi Leena E. Azhar Mohammed Moulay Steve Harakeh Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes
description	Abstract In this study, two highly thermotolerant and methanol-tolerant lipase-producing bacteria were isolated from cooking oil and they exhibited a high number of catalytic lipase activities recording 18.65 ± 0.68 U/mL and 13.14 ± 0.03 U/mL, respectively. Bacterial isolates were identified according to phenotypic and genotypic 16S rRNA characterization as Kocuria flava ASU5 (MT919305) and Bacillus circulans ASU11 (MT919306). Lipases produced from Kocuria flava ASU5 showed the highest methanol tolerance, recording 98.4% relative activity as well as exhibited high thermostability and alkaline stability. Under the optimum conditions obtained from 3D plots of response surface methodology design, the Kocuria flava ASU5 biocatalyst exhibited an 83.08% yield of biodiesel at optimized reaction variables of, 60 ○C, pH value 8 and 1:2 oil/alcohol molar ratios in the reaction mixture. As well as, the obtained results showed the interactions of temperature/methanol were significant effects, whereas this was not noted in the case of temperature/pH and pH/methanol interactions. The obtained amount of biodiesel from cooking oil was 83.08%, which was analyzed by a GC/Ms profile. The produced biodiesel was confirmed by Fourier-transform infrared spectroscopy (FTIR) approaches showing an absorption band at 1743 cm−1, which is recognized for its absorption in the carbonyl group (C=O) which is characteristic of ester absorption. The energy content generated from biodiesel synthesized was estimated as 12,628.5 kJ/mol. Consequently, Kocuria flava MT919305 may provide promising thermostable, methanol-tolerant lipases, which may improve the economic feasibility and biotechnology of enzyme biocatalysis in the synthesis of value-added green chemicals.
format	article
author	Azhar Najjar Elhagag Ahmed Hassan Nidal Zabermawi Saber H. Saber Leena H. Bajrai Mohammed S. Almuhayawi Turki S. Abujamel Saad B. Almasaudi Leena E. Azhar Mohammed Moulay Steve Harakeh
author_facet	Azhar Najjar Elhagag Ahmed Hassan Nidal Zabermawi Saber H. Saber Leena H. Bajrai Mohammed S. Almuhayawi Turki S. Abujamel Saad B. Almasaudi Leena E. Azhar Mohammed Moulay Steve Harakeh
author_sort	Azhar Najjar
title	Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes
title_short	Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes
title_full	Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes
title_fullStr	Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes
title_full_unstemmed	Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes
title_sort	optimizing the catalytic activities of methanol and thermotolerant kocuria flava lipases for biodiesel production from cooking oil wastes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1c49380b4a8547e08d0a4ffd225faa7f
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Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

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