RNA is an integral component of chromatin that contributes to its structural organization.

Chromatin structure is influenced by multiples factors, such as pH, temperature, nature and concentration of counterions, post-translational modifications of histones and binding of structural non-histone proteins. RNA is also known to contribute to the regulation of chromatin structure as chromatin...

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Autores principales:	Antonio Rodríguez-Campos, Fernando Azorín
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c51caae29de4731a64c33554e498543
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spelling	oai:doaj.org-article:1c51caae29de4731a64c33554e4985432021-11-25T06:10:28ZRNA is an integral component of chromatin that contributes to its structural organization.1932-620310.1371/journal.pone.0001182https://doaj.org/article/1c51caae29de4731a64c33554e4985432007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001182https://doaj.org/toc/1932-6203Chromatin structure is influenced by multiples factors, such as pH, temperature, nature and concentration of counterions, post-translational modifications of histones and binding of structural non-histone proteins. RNA is also known to contribute to the regulation of chromatin structure as chromatin-induced gene silencing was shown to depend on the RNAi machinery in S. pombe, plants and Drosophila. Moreover, both in Drosophila and mammals, dosage compensation requires the contribution of specific non-coding RNAs. However, whether RNA itself plays a direct structural role in chromatin is not known. Here, we report results that indicate a general structural role for RNA in eukaryotic chromatin. RNA is found associated to purified chromatin prepared from chicken liver, or cultured Drosophila S2 cells, and treatment with RNase A alters the structural properties of chromatin. Our results indicate that chromatin-associated RNAs, which account for 2%-5% of total chromatin-associated nucleic acids, are polyA(-) and show a size similar to that of the DNA contained in the corresponding chromatin fragments. Chromatin-associated RNA(s) are not likely to correspond to nascent transcripts as they are also found bound to chromatin when cells are treated with alpha-amanitin. After treatment with RNase A, chromatin fragments of molecular weight >3.000 bp of DNA showed reduced sedimentation through sucrose gradients and increased sensitivity to micrococcal nuclease digestion. This structural transition, which is observed both at euchromatic and heterochromatic regions, proceeds without loss of histone H1 or any significant change in core-histone composition and integrity.Antonio Rodríguez-CamposFernando AzorínPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 11, p e1182 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antonio Rodríguez-Campos Fernando Azorín RNA is an integral component of chromatin that contributes to its structural organization.
description	Chromatin structure is influenced by multiples factors, such as pH, temperature, nature and concentration of counterions, post-translational modifications of histones and binding of structural non-histone proteins. RNA is also known to contribute to the regulation of chromatin structure as chromatin-induced gene silencing was shown to depend on the RNAi machinery in S. pombe, plants and Drosophila. Moreover, both in Drosophila and mammals, dosage compensation requires the contribution of specific non-coding RNAs. However, whether RNA itself plays a direct structural role in chromatin is not known. Here, we report results that indicate a general structural role for RNA in eukaryotic chromatin. RNA is found associated to purified chromatin prepared from chicken liver, or cultured Drosophila S2 cells, and treatment with RNase A alters the structural properties of chromatin. Our results indicate that chromatin-associated RNAs, which account for 2%-5% of total chromatin-associated nucleic acids, are polyA(-) and show a size similar to that of the DNA contained in the corresponding chromatin fragments. Chromatin-associated RNA(s) are not likely to correspond to nascent transcripts as they are also found bound to chromatin when cells are treated with alpha-amanitin. After treatment with RNase A, chromatin fragments of molecular weight >3.000 bp of DNA showed reduced sedimentation through sucrose gradients and increased sensitivity to micrococcal nuclease digestion. This structural transition, which is observed both at euchromatic and heterochromatic regions, proceeds without loss of histone H1 or any significant change in core-histone composition and integrity.
format	article
author	Antonio Rodríguez-Campos Fernando Azorín
author_facet	Antonio Rodríguez-Campos Fernando Azorín
author_sort	Antonio Rodríguez-Campos
title	RNA is an integral component of chromatin that contributes to its structural organization.
title_short	RNA is an integral component of chromatin that contributes to its structural organization.
title_full	RNA is an integral component of chromatin that contributes to its structural organization.
title_fullStr	RNA is an integral component of chromatin that contributes to its structural organization.
title_full_unstemmed	RNA is an integral component of chromatin that contributes to its structural organization.
title_sort	rna is an integral component of chromatin that contributes to its structural organization.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/1c51caae29de4731a64c33554e498543
work_keys_str_mv	AT antoniorodriguezcampos rnaisanintegralcomponentofchromatinthatcontributestoitsstructuralorganization AT fernandoazorin rnaisanintegralcomponentofchromatinthatcontributestoitsstructuralorganization
_version_	1718414091008081920

RNA is an integral component of chromatin that contributes to its structural organization.

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