An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.

In methicillin resistant Staphylococcus aureus (MRSA), the arginine catabolic mobile element (ACME) was initially described in USA300 (t008-ST8) where it is located downstream of the staphylococcal cassette chromosome mec (SCCmec). A common health-care associated MRSA in Copenhagen, Denmark (t024-ST...

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Autores principales:	Mette Damkjær Bartels, Lars Hestbjerg Hansen, Kit Boye, Søren J Sørensen, Henrik Westh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c54695760e64cb5b191a90255ba9b6e
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spelling	oai:doaj.org-article:1c54695760e64cb5b191a90255ba9b6e2021-11-18T06:59:55ZAn unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.1932-620310.1371/journal.pone.0016193https://doaj.org/article/1c54695760e64cb5b191a90255ba9b6e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21283578/?tool=EBIhttps://doaj.org/toc/1932-6203In methicillin resistant Staphylococcus aureus (MRSA), the arginine catabolic mobile element (ACME) was initially described in USA300 (t008-ST8) where it is located downstream of the staphylococcal cassette chromosome mec (SCCmec). A common health-care associated MRSA in Copenhagen, Denmark (t024-ST8) is clonally related to USA300 and is frequently PCR positive for the ACME specific arcA-gene. This study is the first to describe an ACME element upstream of the SCCmec in MRSA. By traditional SCCmec typing schemes, the SCCmec of t024-ST8 strain M1 carries SCCmec IVa, but full sequencing of the cassette revealed that the entire J3 region had no homology to published SCCmec IVa. Within the J3 region of M1 was a 1705 bp sequence only similar to a sequence in S. haemolyticus strain JCSC1435 and 2941 bps with no homology found in GenBank. In addition to the usual direct repeats (DR) at each extremity of SCCmec, M1 had two new DR between the orfX gene and the J3 region of the SCCmec. The region between the orfX DR (DR1) and DR2 contained the ccrAB4 genes. An ACME II-like element was located between DR2 and DR3. The entire 26,468 bp sequence between DR1 and DR3 was highly similar to parts of the ACME composite island of S. epidermidis strain ATCC12228. Sequencing of an ACME negative t024-ST8 strain (M299) showed that DR1 and the sequence between DR1 and DR3 was missing. The finding of a mobile ACME II-like element inserted downstream of orfX and upstream of SCCmec indicates a novel recombination between staphylococcal species.Mette Damkjær BartelsLars Hestbjerg HansenKit BoyeSøren J SørensenHenrik WesthPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16193 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mette Damkjær Bartels Lars Hestbjerg Hansen Kit Boye Søren J Sørensen Henrik Westh An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.
description	In methicillin resistant Staphylococcus aureus (MRSA), the arginine catabolic mobile element (ACME) was initially described in USA300 (t008-ST8) where it is located downstream of the staphylococcal cassette chromosome mec (SCCmec). A common health-care associated MRSA in Copenhagen, Denmark (t024-ST8) is clonally related to USA300 and is frequently PCR positive for the ACME specific arcA-gene. This study is the first to describe an ACME element upstream of the SCCmec in MRSA. By traditional SCCmec typing schemes, the SCCmec of t024-ST8 strain M1 carries SCCmec IVa, but full sequencing of the cassette revealed that the entire J3 region had no homology to published SCCmec IVa. Within the J3 region of M1 was a 1705 bp sequence only similar to a sequence in S. haemolyticus strain JCSC1435 and 2941 bps with no homology found in GenBank. In addition to the usual direct repeats (DR) at each extremity of SCCmec, M1 had two new DR between the orfX gene and the J3 region of the SCCmec. The region between the orfX DR (DR1) and DR2 contained the ccrAB4 genes. An ACME II-like element was located between DR2 and DR3. The entire 26,468 bp sequence between DR1 and DR3 was highly similar to parts of the ACME composite island of S. epidermidis strain ATCC12228. Sequencing of an ACME negative t024-ST8 strain (M299) showed that DR1 and the sequence between DR1 and DR3 was missing. The finding of a mobile ACME II-like element inserted downstream of orfX and upstream of SCCmec indicates a novel recombination between staphylococcal species.
format	article
author	Mette Damkjær Bartels Lars Hestbjerg Hansen Kit Boye Søren J Sørensen Henrik Westh
author_facet	Mette Damkjær Bartels Lars Hestbjerg Hansen Kit Boye Søren J Sørensen Henrik Westh
author_sort	Mette Damkjær Bartels
title	An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.
title_short	An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.
title_full	An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.
title_fullStr	An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.
title_full_unstemmed	An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.
title_sort	unexpected location of the arginine catabolic mobile element (acme) in a usa300-related mrsa strain.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1c54695760e64cb5b191a90255ba9b6e
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An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.

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