Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood

Abstract Archival tissue samples collected longitudinally from a patient who died from HPV16-induced high-grade anal intraepithelial squamous cell carcinoma with vertebral HPV16–positive metastasis were retrospectively analyzed by the Capture-HPV method (Capt-HPV) followed by Next-Generation Sequenc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hélène Péré, Raphael Vernet, Simon Pernot, Juliette Pavie, Nicolas Robillard, Julien Puech, Sonia Lameiras, Marie-Laure Lucas, Alain Nicolas, Cécile Badoual, Bastien Rance, Laurent Bélec, Laurence Weiss, Maxime Wack, David Veyer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c5ad95f84864e86a239366422da5ac1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1c5ad95f84864e86a239366422da5ac1
record_format	dspace
spelling	oai:doaj.org-article:1c5ad95f84864e86a239366422da5ac12021-12-02T11:35:52ZEpisomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood10.1038/s41598-021-84110-22045-2322https://doaj.org/article/1c5ad95f84864e86a239366422da5ac12021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84110-2https://doaj.org/toc/2045-2322Abstract Archival tissue samples collected longitudinally from a patient who died from HPV16-induced high-grade anal intraepithelial squamous cell carcinoma with vertebral HPV16–positive metastasis were retrospectively analyzed by the Capture-HPV method (Capt-HPV) followed by Next-Generation Sequencing (NGS). Full length nucleotide sequences of the same HPV16 were identified from the initial and second anal biopsy samples, from plasma sample and from vertebral metastasis biopsy. Remarkably, HPV was episomal in each sample. The HPV genome sequence was closest to the HPV16 Qv18158E variant subtype (A1 lineage) exhibiting base substitutions and deletions in 7 and 2 HPV loci, respectively. In conclusion, the powerful Capt-HPV followed by NGS allows evidencing the detailed cartography of tumoral and circulating HPV DNA, giving rise to a unique and unexpected episomal virus molecular status in a context of aggressive carcinoma, underlying the importance of HPV status and its association with clinical features for further prospective studies.Hélène PéréRaphael VernetSimon PernotJuliette PavieNicolas RobillardJulien PuechSonia LameirasMarie-Laure LucasAlain NicolasCécile BadoualBastien RanceLaurent BélecLaurence WeissMaxime WackDavid VeyerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hélène Péré Raphael Vernet Simon Pernot Juliette Pavie Nicolas Robillard Julien Puech Sonia Lameiras Marie-Laure Lucas Alain Nicolas Cécile Badoual Bastien Rance Laurent Bélec Laurence Weiss Maxime Wack David Veyer Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood
description	Abstract Archival tissue samples collected longitudinally from a patient who died from HPV16-induced high-grade anal intraepithelial squamous cell carcinoma with vertebral HPV16–positive metastasis were retrospectively analyzed by the Capture-HPV method (Capt-HPV) followed by Next-Generation Sequencing (NGS). Full length nucleotide sequences of the same HPV16 were identified from the initial and second anal biopsy samples, from plasma sample and from vertebral metastasis biopsy. Remarkably, HPV was episomal in each sample. The HPV genome sequence was closest to the HPV16 Qv18158E variant subtype (A1 lineage) exhibiting base substitutions and deletions in 7 and 2 HPV loci, respectively. In conclusion, the powerful Capt-HPV followed by NGS allows evidencing the detailed cartography of tumoral and circulating HPV DNA, giving rise to a unique and unexpected episomal virus molecular status in a context of aggressive carcinoma, underlying the importance of HPV status and its association with clinical features for further prospective studies.
format	article
author	Hélène Péré Raphael Vernet Simon Pernot Juliette Pavie Nicolas Robillard Julien Puech Sonia Lameiras Marie-Laure Lucas Alain Nicolas Cécile Badoual Bastien Rance Laurent Bélec Laurence Weiss Maxime Wack David Veyer
author_facet	Hélène Péré Raphael Vernet Simon Pernot Juliette Pavie Nicolas Robillard Julien Puech Sonia Lameiras Marie-Laure Lucas Alain Nicolas Cécile Badoual Bastien Rance Laurent Bélec Laurence Weiss Maxime Wack David Veyer
author_sort	Hélène Péré
title	Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood
title_short	Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood
title_full	Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood
title_fullStr	Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood
title_full_unstemmed	Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood
title_sort	episomal hpv16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1c5ad95f84864e86a239366422da5ac1
work_keys_str_mv	AT helenepere episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT raphaelvernet episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT simonpernot episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT juliettepavie episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT nicolasrobillard episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT julienpuech episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT sonialameiras episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT marielaurelucas episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT alainnicolas episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT cecilebadoual episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT bastienrance episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT laurentbelec episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT laurenceweiss episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT maximewack episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood AT davidveyer episomalhpv16responsibleforaggressiveanddeadlymetastaticanalsquamouscellcarcinomaevidencedinperipheralblood
_version_	1718395806686380032

Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood

Ejemplares similares