Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference

Abstract Measurement of cardiac troponin I (cTnI) should be feasible for point-of-care testing (POCT) to diagnose acute myocardial infarction (AMI). Lateral flow immunoassays (LFIAs) have been long implemented in POCT and clinical settings. However, sensitivity, matrix effect and quantitation in lat...

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Autores principales:	Sherif Bayoumy, Iida Martiskainen, Taina Heikkilä, Carita Rautanen, Pirjo Hedberg, Heidi Hyytiä, Saara Wittfooth, Kim Pettersson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c5b42e46de54f6fbac46bc0d21ec2a3
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spelling	oai:doaj.org-article:1c5b42e46de54f6fbac46bc0d21ec2a32021-12-02T17:26:49ZSensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference10.1038/s41598-021-98199-y2045-2322https://doaj.org/article/1c5b42e46de54f6fbac46bc0d21ec2a32021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98199-yhttps://doaj.org/toc/2045-2322Abstract Measurement of cardiac troponin I (cTnI) should be feasible for point-of-care testing (POCT) to diagnose acute myocardial infarction (AMI). Lateral flow immunoassays (LFIAs) have been long implemented in POCT and clinical settings. However, sensitivity, matrix effect and quantitation in lateral flow immunoassays (LFIAs) have been major limiting factors. The performance of LFIAs can be improved with upconverting nanoparticle (UCNP) reporters. Here we report a new methodological approach to quantify cTnI using UCNP-LFIA technology with minimized plasma interference. The performance of the developed UCNP-LFIA was evaluated using clinical plasma samples (n = 262). The developed UCNP-LFIA was compared to two reference assays, the Siemens Advia Centaur assay and an in-house well-based cTnI assay. By introducing an anti-IgM scrub line and dried EDTA in the LFIA strip, the detection of cTnI in plasma samples was fully recovered. The UCNP-LFIA was able to quantify cTnI concentrations in patient samples within the range of 30–10,000 ng/L. The LoB and LoD of the UCNP-LFIA were 8.4 ng/L and 30 ng/L. The method comparisons showed good correlation (Spearman’s correlation 0.956 and 0.949, p < 0.0001). The developed UCNP-LFIA had LoD suitable for ruling in AMI in patients with elevated cTnI levels and was able to quantify cTnI concentrations in patient samples. The technology has potential to provide simple and rapid assay for POCT in ED settingSherif BayoumyIida MartiskainenTaina HeikkiläCarita RautanenPirjo HedbergHeidi HyytiäSaara WittfoothKim PetterssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sherif Bayoumy Iida Martiskainen Taina Heikkilä Carita Rautanen Pirjo Hedberg Heidi Hyytiä Saara Wittfooth Kim Pettersson Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference
description	Abstract Measurement of cardiac troponin I (cTnI) should be feasible for point-of-care testing (POCT) to diagnose acute myocardial infarction (AMI). Lateral flow immunoassays (LFIAs) have been long implemented in POCT and clinical settings. However, sensitivity, matrix effect and quantitation in lateral flow immunoassays (LFIAs) have been major limiting factors. The performance of LFIAs can be improved with upconverting nanoparticle (UCNP) reporters. Here we report a new methodological approach to quantify cTnI using UCNP-LFIA technology with minimized plasma interference. The performance of the developed UCNP-LFIA was evaluated using clinical plasma samples (n = 262). The developed UCNP-LFIA was compared to two reference assays, the Siemens Advia Centaur assay and an in-house well-based cTnI assay. By introducing an anti-IgM scrub line and dried EDTA in the LFIA strip, the detection of cTnI in plasma samples was fully recovered. The UCNP-LFIA was able to quantify cTnI concentrations in patient samples within the range of 30–10,000 ng/L. The LoB and LoD of the UCNP-LFIA were 8.4 ng/L and 30 ng/L. The method comparisons showed good correlation (Spearman’s correlation 0.956 and 0.949, p < 0.0001). The developed UCNP-LFIA had LoD suitable for ruling in AMI in patients with elevated cTnI levels and was able to quantify cTnI concentrations in patient samples. The technology has potential to provide simple and rapid assay for POCT in ED setting
format	article
author	Sherif Bayoumy Iida Martiskainen Taina Heikkilä Carita Rautanen Pirjo Hedberg Heidi Hyytiä Saara Wittfooth Kim Pettersson
author_facet	Sherif Bayoumy Iida Martiskainen Taina Heikkilä Carita Rautanen Pirjo Hedberg Heidi Hyytiä Saara Wittfooth Kim Pettersson
author_sort	Sherif Bayoumy
title	Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference
title_short	Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference
title_full	Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference
title_fullStr	Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference
title_full_unstemmed	Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference
title_sort	sensitive and quantitative detection of cardiac troponin i with upconverting nanoparticle lateral flow test with minimized interference
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1c5b42e46de54f6fbac46bc0d21ec2a3
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Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference

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