Comparison of In Vivo and In Vitro Digestibility in Rabbits
The apparent dry matter digestibility of diets for rabbits was measured in vivo (ADMD<sub>vv</sub>) and in vitro with the Ankom Daisy<sup>II</sup> Incubator. Four diets were tested: low fiber (LF), LF + 5% of pregerminated fenugreek seeds (PGFS) (LF5), LF + 10% PGFS (LF10), a...
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MDPI AG
2021
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oai:doaj.org-article:1c64928834ba4371b8e78ecb5d3b81af2021-11-25T16:20:02ZComparison of In Vivo and In Vitro Digestibility in Rabbits10.3390/ani111132672076-2615https://doaj.org/article/1c64928834ba4371b8e78ecb5d3b81af2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3267https://doaj.org/toc/2076-2615The apparent dry matter digestibility of diets for rabbits was measured in vivo (ADMD<sub>vv</sub>) and in vitro with the Ankom Daisy<sup>II</sup> Incubator. Four diets were tested: low fiber (LF), LF + 5% of pregerminated fenugreek seeds (PGFS) (LF5), LF + 10% PGFS (LF10), and high fiber (HF). For the ADMD<sub>vv</sub>, feces samples were collected from 56 White New-Zealand × Californian rabbits fed the 4 diets; animals were randomly allocated into 4 groups and housed in individual cages. For the in vitro trial, 3 methods were tested: fecal inoculum (FA) with Kansans State buffer; fecal inoculum with artificial saliva (FB); and multienzyme (ENZ). Fecal inocula were collected at slaughtering from the distal colon of rabbits fed ad libitum the LF diet. For FA and FB methods, the digestibility was measured at 36 and 48 h. The in vitro methods ranked the apparent dry matter digestibility of diets in the same order as in vivo, but ENZ values were always higher than FA and FB at 36 and 48 h. The prediction equations of in vivo digestibility with the ENZ method showed higher coefficient of determination (R<sup>2</sup> = 0.69) and lower SE (0.190) than FA and FB; also, reproducibility was higher with ENZ (CV = 3.1%). In conclusion, different methods can be applied to the Ankom Daisy<sup>II</sup> Incubator to study the digestibility in rabbits. In our trial, the better reproducibility was observed with the multienzyme method than FA and FB were probably related to the variability of inocula.Sonia TassoneRiccardo FortinaSabah MabroukiYasser HachanaSalvatore BarberaMDPI AGarticlerabbit in vitro digestibilityAnkom Daisy<sup>II</sup> Incubatorenzymatic techniquefecal inoculumVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3267, p 3267 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
rabbit in vitro digestibility Ankom Daisy<sup>II</sup> Incubator enzymatic technique fecal inoculum Veterinary medicine SF600-1100 Zoology QL1-991 |
| spellingShingle |
rabbit in vitro digestibility Ankom Daisy<sup>II</sup> Incubator enzymatic technique fecal inoculum Veterinary medicine SF600-1100 Zoology QL1-991 Sonia Tassone Riccardo Fortina Sabah Mabrouki Yasser Hachana Salvatore Barbera Comparison of In Vivo and In Vitro Digestibility in Rabbits |
| description |
The apparent dry matter digestibility of diets for rabbits was measured in vivo (ADMD<sub>vv</sub>) and in vitro with the Ankom Daisy<sup>II</sup> Incubator. Four diets were tested: low fiber (LF), LF + 5% of pregerminated fenugreek seeds (PGFS) (LF5), LF + 10% PGFS (LF10), and high fiber (HF). For the ADMD<sub>vv</sub>, feces samples were collected from 56 White New-Zealand × Californian rabbits fed the 4 diets; animals were randomly allocated into 4 groups and housed in individual cages. For the in vitro trial, 3 methods were tested: fecal inoculum (FA) with Kansans State buffer; fecal inoculum with artificial saliva (FB); and multienzyme (ENZ). Fecal inocula were collected at slaughtering from the distal colon of rabbits fed ad libitum the LF diet. For FA and FB methods, the digestibility was measured at 36 and 48 h. The in vitro methods ranked the apparent dry matter digestibility of diets in the same order as in vivo, but ENZ values were always higher than FA and FB at 36 and 48 h. The prediction equations of in vivo digestibility with the ENZ method showed higher coefficient of determination (R<sup>2</sup> = 0.69) and lower SE (0.190) than FA and FB; also, reproducibility was higher with ENZ (CV = 3.1%). In conclusion, different methods can be applied to the Ankom Daisy<sup>II</sup> Incubator to study the digestibility in rabbits. In our trial, the better reproducibility was observed with the multienzyme method than FA and FB were probably related to the variability of inocula. |
| format |
article |
| author |
Sonia Tassone Riccardo Fortina Sabah Mabrouki Yasser Hachana Salvatore Barbera |
| author_facet |
Sonia Tassone Riccardo Fortina Sabah Mabrouki Yasser Hachana Salvatore Barbera |
| author_sort |
Sonia Tassone |
| title |
Comparison of In Vivo and In Vitro Digestibility in Rabbits |
| title_short |
Comparison of In Vivo and In Vitro Digestibility in Rabbits |
| title_full |
Comparison of In Vivo and In Vitro Digestibility in Rabbits |
| title_fullStr |
Comparison of In Vivo and In Vitro Digestibility in Rabbits |
| title_full_unstemmed |
Comparison of In Vivo and In Vitro Digestibility in Rabbits |
| title_sort |
comparison of in vivo and in vitro digestibility in rabbits |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/1c64928834ba4371b8e78ecb5d3b81af |
| work_keys_str_mv |
AT soniatassone comparisonofinvivoandinvitrodigestibilityinrabbits AT riccardofortina comparisonofinvivoandinvitrodigestibilityinrabbits AT sabahmabrouki comparisonofinvivoandinvitrodigestibilityinrabbits AT yasserhachana comparisonofinvivoandinvitrodigestibilityinrabbits AT salvatorebarbera comparisonofinvivoandinvitrodigestibilityinrabbits |
| _version_ |
1718413184471138304 |