Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand

Abstract. Vanijajiva O. 2020. Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand. Biodiversitas 21: 666-673. Manilkara consists of four species, namely, Manilkara hexandra, M. kauki, M. littoralis, and M. zapota in Thailand. Particularly, M. zapota (sapodilla...

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Autor principal:	Ongkarn Vanijajiva
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	genetic diversity manilkara sapodilla scot thailand Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1c6ee212eee64b1fa71240486bd00abf
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spelling	oai:doaj.org-article:1c6ee212eee64b1fa71240486bd00abf2021-11-21T22:01:10ZStart codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand1412-033X2085-472210.13057/biodiv/d210232https://doaj.org/article/1c6ee212eee64b1fa71240486bd00abf2020-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5153https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Vanijajiva O. 2020. Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand. Biodiversitas 21: 666-673. Manilkara consists of four species, namely, Manilkara hexandra, M. kauki, M. littoralis, and M. zapota in Thailand. Particularly, M. zapota (sapodilla), commonly known as Lamut in Thailand, is one of commercially notable edible fruits continues to be a highly valued tree and the fruits are a source of food and income. The objective of this research was to determine the diversity and relationship of Manilkara accessions derived from districts in Thailand based on DNA profiles. Genetic diversity and relationship of all accessions were investigated using Start Codon Targeted (SCoT) marker analysis. Of 30 SCoT primers screened, 27 SCoT primers were identified to be polymorphism. A total of 176 DNA bands with size varied from 120 bp to 1200 bp were amplified, and 101 (57.38%) of them were polymorphic. Based on similarity coefficients, total of 36 accessions were clustered into two groups. The cluster of polymorphism generated by SCoT was associated with their locality and morphological characters. Therefore, the present data provide high-valued information for the management of germplasm, genetic improvement, and conservation of the genetic resources of Manilkara accessions.Ongkarn VanijajivaMBI & UNS Soloarticlegenetic diversitymanilkarasapodillascotthailandBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic diversity manilkara sapodilla scot thailand Biology (General) QH301-705.5
spellingShingle	genetic diversity manilkara sapodilla scot thailand Biology (General) QH301-705.5 Ongkarn Vanijajiva Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand
description	Abstract. Vanijajiva O. 2020. Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand. Biodiversitas 21: 666-673. Manilkara consists of four species, namely, Manilkara hexandra, M. kauki, M. littoralis, and M. zapota in Thailand. Particularly, M. zapota (sapodilla), commonly known as Lamut in Thailand, is one of commercially notable edible fruits continues to be a highly valued tree and the fruits are a source of food and income. The objective of this research was to determine the diversity and relationship of Manilkara accessions derived from districts in Thailand based on DNA profiles. Genetic diversity and relationship of all accessions were investigated using Start Codon Targeted (SCoT) marker analysis. Of 30 SCoT primers screened, 27 SCoT primers were identified to be polymorphism. A total of 176 DNA bands with size varied from 120 bp to 1200 bp were amplified, and 101 (57.38%) of them were polymorphic. Based on similarity coefficients, total of 36 accessions were clustered into two groups. The cluster of polymorphism generated by SCoT was associated with their locality and morphological characters. Therefore, the present data provide high-valued information for the management of germplasm, genetic improvement, and conservation of the genetic resources of Manilkara accessions.
format	article
author	Ongkarn Vanijajiva
author_facet	Ongkarn Vanijajiva
author_sort	Ongkarn Vanijajiva
title	Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand
title_short	Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand
title_full	Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand
title_fullStr	Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand
title_full_unstemmed	Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand
title_sort	start codon targeted (scot) polymorphism reveals genetic diversity of manilkara in thailand
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/1c6ee212eee64b1fa71240486bd00abf
work_keys_str_mv	AT ongkarnvanijajiva startcodontargetedscotpolymorphismrevealsgeneticdiversityofmanilkarainthailand
_version_	1718418567903313920

Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand

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