Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis

The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB...

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Autores principales:	C Rajamanickam, K Rajmohan
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2012
Materias:	banana ecotypes rapd molecular markers d2 analysis genetic divergence Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/1c73c07ca96b4fd694a91f177cfd2beb
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spelling	oai:doaj.org-article:1c73c07ca96b4fd694a91f177cfd2beb2021-12-02T08:01:08ZDiversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis0973-354X2582-4899https://doaj.org/article/1c73c07ca96b4fd694a91f177cfd2beb2012-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/385https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB-04, OPB-10 and OPB-12 were used. These yielded 46 scorable bands with an average of 7.66 bands per primer. RAPD data were analyzed statistically and a dendrogram was constructed. Twenty three characters were observed in the twenty eight banana ecotypes and were statistically analyzed as per the method proposed by Mahalanobis (D2). From RAPD dendrogram, it was found that the banana clones clustered into fourteen groups at a distance of 0.200. At a distance of 0.250, 8 out of 12 Nendran (AAB group) ecotypes formed a single cluster at the same distance. Among Palayankodan (AAB group) ecotypes PKNNR, Pisang Ceylon, Motta Poovan, Chandra Bale and Palode Palayankodan grouped together and formed a single cluster. Attu Nendran, Monthan, Robusta, Koonoor Ethan, Ilavazha and Vellapalayankodan formed individual clusters and had maximum genetic divergence. Among diploid clones, Ilavazha (BB group) had maximum genetic divergence. Among triploid clones, Attu Nendran, Robusta, Koonoor Ethan and Vellapalayankodan showed maximum genetic divergence. Among Nendran (AAB group) ecotypes, Attu Nendran and Koonoor Ethan revealed maximum genetic divergence. Among Palayankodan (AAB group) ecotypes, Vellapalayankodan recorded the highest genetic divergence. In D2 analysis too, a similar trend was observed.C RajamanickamK RajmohanSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlebananaecotypesrapdmolecular markersd2 analysisgenetic divergencePlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 7, Iss 1, Pp 34-40 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	banana ecotypes rapd molecular markers d2 analysis genetic divergence Plant culture SB1-1110
spellingShingle	banana ecotypes rapd molecular markers d2 analysis genetic divergence Plant culture SB1-1110 C Rajamanickam K Rajmohan Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
description	The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB-04, OPB-10 and OPB-12 were used. These yielded 46 scorable bands with an average of 7.66 bands per primer. RAPD data were analyzed statistically and a dendrogram was constructed. Twenty three characters were observed in the twenty eight banana ecotypes and were statistically analyzed as per the method proposed by Mahalanobis (D2). From RAPD dendrogram, it was found that the banana clones clustered into fourteen groups at a distance of 0.200. At a distance of 0.250, 8 out of 12 Nendran (AAB group) ecotypes formed a single cluster at the same distance. Among Palayankodan (AAB group) ecotypes PKNNR, Pisang Ceylon, Motta Poovan, Chandra Bale and Palode Palayankodan grouped together and formed a single cluster. Attu Nendran, Monthan, Robusta, Koonoor Ethan, Ilavazha and Vellapalayankodan formed individual clusters and had maximum genetic divergence. Among diploid clones, Ilavazha (BB group) had maximum genetic divergence. Among triploid clones, Attu Nendran, Robusta, Koonoor Ethan and Vellapalayankodan showed maximum genetic divergence. Among Nendran (AAB group) ecotypes, Attu Nendran and Koonoor Ethan revealed maximum genetic divergence. Among Palayankodan (AAB group) ecotypes, Vellapalayankodan recorded the highest genetic divergence. In D2 analysis too, a similar trend was observed.
format	article
author	C Rajamanickam K Rajmohan
author_facet	C Rajamanickam K Rajmohan
author_sort	C Rajamanickam
title	Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
title_short	Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
title_full	Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
title_fullStr	Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
title_full_unstemmed	Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
title_sort	diversity studies in ecotypes of banana (musa spp.) using molecular markers and d2 analysis
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2012
url	https://doaj.org/article/1c73c07ca96b4fd694a91f177cfd2beb
work_keys_str_mv	AT crajamanickam diversitystudiesinecotypesofbananamusasppusingmolecularmarkersandd2analysis AT krajmohan diversitystudiesinecotypesofbananamusasppusingmolecularmarkersandd2analysis
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Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis

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